Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for detecting lactulose in dairy product

A detection method and technology for dairy products, applied in measurement devices, instruments, scientific instruments, etc., can solve the problems of inability to accurately quantify lactulose content, low lactulose sensitivity, and high detection limit, and achieve inhibition of isomerization, The effect of increasing sensitivity and increasing usage

Active Publication Date: 2020-10-30
唐山市食品药品综合检验检测中心
View PDF1 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the detection of lactulose by liquid chromatography tandem mass spectrometer has low sensitivity, high detection limit, and is easily interfered by lactose, and there are many optical isomers in lactulose, so direct measurement cannot accurately quantify the content of lactulose

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for detecting lactulose in dairy product
  • Method for detecting lactulose in dairy product
  • Method for detecting lactulose in dairy product

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] Embodiment 1: Add the lactulose standard solution into the fresh milk sample that has been measured, and mix well to obtain the lactulose spiked sample of this embodiment.

[0045] (1) Sample pretreatment

[0046] Draw 5.00mL lactulose spiked sample into a 50mL centrifuge tube, add 5.0mL water, 2.0mL potassium ferrocyanide solution with a concentration of 130g / L, 2.0mL zinc sulfate solution with a concentration of 168g / L and 6.0mL phosphate For salt buffer (48 g / L disodium hydrogen phosphate, 8.6 g / L sodium dihydrogen phosphate, 1 g / L magnesium sulfate, adjust the pH to 7.5), every time a reagent is added, it needs to be vortexed and mixed thoroughly. After mixing well, centrifuge at 8000r / min at 4°C for 5min, take 1.0mL supernatant, add 4.0mL phosphate buffer (disodium hydrogen phosphate 48g / L, sodium dihydrogen phosphate 8.6g / L, magnesium sulfate 1g / L, adjust the pH to 7.5), mix well to obtain a diluent. Aspirate 1.00 mL of the diluent, add 200 μL of β-D-galactosid...

Embodiment 2

[0060] Prepare pasteurized milk samples.

[0061] (1) Sample pretreatment

[0062] Draw 5.00mL pasteurized milk sample into a 50mL centrifuge tube, add 5.0mL water, 2.0mL potassium ferrocyanide solution with a concentration of 130g / L, 2.0mL zinc sulfate solution with a concentration of 168g / L and 6.0mL phosphate For salt buffer (48 g / L disodium hydrogen phosphate, 8.6 g / L sodium dihydrogen phosphate, 1 g / L magnesium sulfate, adjust the pH to 7.5), every time a reagent is added, it needs to be vortexed and mixed thoroughly. After mixing, centrifuge at 8000r / min at 4°C for 5min, take 1.0mL supernatant, add 4.0mL phosphate buffer (disodium hydrogen phosphate 48g / L, sodium dihydrogen phosphate 8.6g / L, magnesium sulfate 1g / L L, adjust the pH to 7.5), and mix well. Aspirate 1.0 mL of the diluent, add 0.2 mL of β-D-galactosidase suspension (enzyme concentration: 40 mg / mL), shake slightly, and perform enzymatic reaction at 50°C for 1 h.

[0063] Take 0.60 mL of enzymatic hydrolysis...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides a method for detecting lactulose in a dairy product. The method comprises the steps of adding beta-D-galactosidase into a dairy product sample, carrying out an enzymatic hydrolysis reaction on lactose and lactulose to generate galactose, fructose and glucose, respectively carrying out an oximation reaction on galactose, glucose and fructose and methoxylamine, and carrying out a derivatization reaction on the generated reactants and N-methyl-N-trimethylsilane trifluoroacetamide to finally obtain a test solution. The test sample solution is analyzed by adopting an ultra-high performance liquid chromatography tandem mass spectrometer to obtain the content of lactulose. The method can accurately and sensitively determine the content of lactulose in pasteurized milk.

Description

technical field [0001] The invention belongs to the field of lactulose detection, in particular to a method for detecting lactulose in dairy products. Background technique [0002] Liquid milk is usually sterilized by various heat treatments during production, and nutrients are also destroyed during the heating process. Many countries and international organizations use some indicators related to heat treatment intensity to estimate the degree of heat damage of liquid milk to evaluate the processing quality of liquid milk, and lactulose is an important heat-sensitive index produced during processing. [0003] Existing analysis methods for lactulose mainly include enzymatic method, ion chromatography, etc., because pasteurized milk contains a lot of lactose, and lactose and lactulose are isomers, so the increase in the determination of lactulose Increased the difficulty of separation and qualitative quantification. With the popularity of liquid chromatography tandem mass sp...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06G01N30/86
CPCG01N30/02G01N30/06G01N30/8682G01N2030/045G01N2030/065
Inventor 周鑫张宁郑百芹王磊康俊杰张丽芳肖琎张鑫项爱丽段晓然毕振辉
Owner 唐山市食品药品综合检验检测中心
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com