Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

A kind of detection method of lactulose in dairy products

A detection method and technology for dairy products, applied in measurement devices, instruments, scientific instruments, etc., can solve the problems of inability to accurately quantify lactulose content, low lactulose sensitivity, and high detection limit, and achieve inhibition of isomerization, The effect of increasing sensitivity and increasing usage

Active Publication Date: 2022-07-29
唐山市食品药品综合检验检测中心
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the detection of lactulose by liquid chromatography tandem mass spectrometer has low sensitivity, high detection limit, and is easily interfered by lactose, and there are many optical isomers in lactulose, so direct measurement cannot accurately quantify the content of lactulose

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A kind of detection method of lactulose in dairy products
  • A kind of detection method of lactulose in dairy products
  • A kind of detection method of lactulose in dairy products

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] Example 1: adding the lactulose standard solution to the measured raw fresh milk sample, and mixing to obtain the lactulose spiked sample of this embodiment.

[0045] (1) Sample pretreatment

[0046]Pipette 5.00 mL of lactulose spiked sample into a 50 mL centrifuge tube, add 5.0 mL of water, 2.0 mL of potassium ferrocyanide solution with a concentration of 130 g / L, 2.0 mL of zinc sulfate solution with a concentration of 168 g / L, and 6.0 mL of phosphate buffer (disodium hydrogen phosphate 48 g / L, sodium dihydrogen phosphate 8.6 g / L, magnesium sulfate 1 g / L, adjusted to pH 7.5), each reagent added, need to be vortexed to mix thoroughly. After thorough mixing, centrifuge at 8000 r / min for 5 min at 4 °C, take 1.0 mL of supernatant, add 4.0 mL of phosphate buffer (disodium hydrogen phosphate 48 g / L, sodium dihydrogen phosphate 8.6 g / L, sulfuric acid Magnesium 1 g / L, adjust pH to 7.5), mix well to obtain a dilution. Aspirate 1.00 mL of the diluent, add 200 μL of β-D-galacto...

Embodiment 2

[0060] Prepare pasteurized milk samples.

[0061] (1) Sample pretreatment

[0062] Pipette 5.00 mL of pasteurized milk sample into a 50 mL centrifuge tube, add 5.0 mL of water, 2.0 mL of potassium ferrocyanide solution with a concentration of 130 g / L, 2.0 mL of zinc sulfate solution with a concentration of 168 g / L, and 6.0 mL of phosphate buffer (disodium hydrogen phosphate 48 g / L, sodium dihydrogen phosphate 8.6 g / L, magnesium sulfate 1 g / L, adjusted to pH 7.5), each reagent added, need to be vortexed to mix thoroughly. After mixing, centrifuge at 8000 r / min for 5 min at 4 °C, take 1.0 mL of supernatant, add 4.0 mL of phosphate buffer (48 g / L disodium hydrogen phosphate, 8.6 g / L sodium dihydrogen phosphate, sulfuric acid Magnesium 1 g / L, adjust pH to 7.5), mix well. Aspirate 1.0 mL of the diluent, add 0.2 mL of β-D-galactosidase suspension (enzyme concentration of 40 mg / mL), shake gently, and perform the enzymatic hydrolysis reaction at 50 °C for 1 h.

[0063] Take 0.60 mL...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides a method for detecting lactulose in dairy products, comprising: adding β-D-galactosidase to a dairy product sample, and reacting lactose and lactulose by enzymatic hydrolysis to generate galactose, fructose and glucose, galactose, glucose After oximation reaction with fructose and methoxyamine respectively, the generated reactant is then subjected to derivatization reaction with N-methyl-N-trimethylsilane trifluoroacetamide to finally obtain a test solution. The test sample liquid was analyzed by ultra-high performance liquid chromatography tandem mass spectrometer to obtain the content of lactulose. The method can accurately and sensitively determine the lactulose content in pasteurized milk.

Description

technical field [0001] The invention belongs to the field of lactulose detection, and particularly relates to a detection method of lactulose in dairy products. Background technique [0002] Liquid milk is usually sterilized during production using various heat treatments, which also destroy nutrients. Many countries and international organizations have selected some indicators related to heat treatment intensity to estimate the degree of thermal damage of liquid milk to evaluate the processing quality of liquid milk, and lactulose is an important heat sensitive indicator produced during processing. [0003] The existing analytical methods for lactulose mainly include enzymatic method, ion chromatography, etc. Since pasteurized milk contains a lot of lactose, and lactose and lactulose are isomers, the increase in lactulose is increased during the determination of lactulose. The difficulty of separation and qualitative and quantitative analysis is greatly increased. With th...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02G01N30/06G01N30/86
CPCG01N30/02G01N30/06G01N30/8682G01N2030/045G01N2030/065
Inventor 周鑫张宁郑百芹王磊康俊杰张丽芳肖琎张鑫项爱丽段晓然毕振辉
Owner 唐山市食品药品综合检验检测中心
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com