Application of antibacterial peptide to preparation of medicines or cosmetics

An antibacterial peptide and cosmetic technology, applied in the field of antibacterial peptides in the preparation of drugs or cosmetics, can solve the problems of no research, no screening and acquisition of antibacterial peptides, etc., and achieve the effect of significant inhibitory activity and low toxicity

Active Publication Date: 2020-11-03
YANTAI HUAXIN BIOPHARM TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Although there are many studies on antimicrobial peptides, antibacterial peptides that can specifically resist Trichophyton rubrum have not been screened, and there are no related studies

Method used

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  • Application of antibacterial peptide to preparation of medicines or cosmetics
  • Application of antibacterial peptide to preparation of medicines or cosmetics
  • Application of antibacterial peptide to preparation of medicines or cosmetics

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Example 1 Screening of anti-trichophyton rubrum bactericidal peptide

[0024] 1. Bacteria culture

[0025] Trichophyton rubrum The 28188 standard strain was planted on a Sabouraud dextrose agar SDA plate, cultured in the dark at 27°C, picked a single colony and put it into a shaker flask of 250mL PDA medium, 150r / min, 27°C dark culture, then 3000r / min, 4°C , centrifuged for 10 min, and the cells were collected to harvest the cells for later use.

[0026] 2. Lipid extraction

[0027] 8 g of the collected bacterial cells were washed and centrifuged repeatedly with PBS, resuspended in 60 mL Tris-HCl buffer (25 mmol / L, pH 7.5), ultrasonically broken, and after centrifugation, 180 mL of chloroform-methanol (1:2, v / v) mixed solvents, mixed and stirred for 100min. Afterwards, 40 mL of chloroform and 40 mL of distilled water were added, and stirring was continued for 30 min. The chloroform phase was separated with a separatory funnel, and the solvent was removed by rotar...

Embodiment 2

[0037] The mensuration of embodiment 2 antimicrobial peptide minimum inhibitory concentration

[0038] The minimum inhibitory concentrations (Minimalinhibitoryconcentrations, MICs) of antimicrobial peptides against indicator bacteria were determined by microdilution method.

[0039] The strains tested were: (1) Escherichia coli ATCC25922; (2) Trichophyton mentagrophytes, Beijing Huayueyang Biotech NRR00060; (3) Trichophyton rubrum 28188. The strains were cultured according to conventional culture methods. After culturing to the logarithmic phase, collect the bacterial cells in the logarithmic phase by centrifugation (3000r / min, 4°C, 10min), wash with sterilized PBS, and resuspend until the OD600 is about 0.3. The peptide was prepared into a 1024 μg / mL stock solution with sterilized PBS, sterilized by filtration with a 0.22 μm filter membrane, and serially diluted with sterilized PBS. Add 100 μL medium, 100 μL test bacteria solution and 50 μL antimicrobial peptide solutions...

Embodiment 3

[0043] The influence of embodiment 3 antimicrobial peptides on the expression of NADPH oxidase

[0044] The 8 μg / mL and 32 μg / mL treated bacteria in Example 2 were collected and soaked in liquid nitrogen to prevent the degradation of RNA, and the samples were ground into as fine a powder as possible using a mortar, grinding rod and liquid nitrogen. Transfer the sample to a 1.5ml pre-cooled centrifuge tube, extract and purify fungal RNA according to the instructions of HiPure Fungal RNA Kit and DNase On Column Kit, and prepare cDNA by reverse transcription. Using primers: select the tubulin of Trichophyton rubrum as internal reference gene F: 5'-CCGCTCTTTGCTCTATTCCT-3, R: 5'-CCATCTCGTCCATACCCTCA-3,) use NADPH oxidase (Nox) gene F: 5'-TGGCTGTGACTTTGACGAGA-3'; R: 5'-CCGACTAACACCGCTACTTC-3' primer for qRT-PCR amplification. The result is as image 3 As shown, compared with the control group, the expression of Nox gene in the antimicrobial peptide treatment group was significantl...

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Abstract

The invention relates to an application of an antibacterial peptide to preparation of medicines or cosmetics. The antibacterial peptide has significant inhibitory activity on human pathogenic fungi namely trichophyton rubrum, and the compound also has the advantage of low toxicity, and therefore, the compound can be used for preparing antifungal medicines and cosmetics.

Description

technical field [0001] The invention belongs to the field of medicines, and in particular relates to the use of an antibacterial peptide in the preparation of medicines or cosmetics. Background technique [0002] Fungal skin disease, also known as dermatophytosis, is an infection caused by superficial fungi invading human skin, hair, and deck, referred to as ringworm. The pathogenic fungi of tinea manuum and tinea pedis are mainly Trichophyton rubrum, Trichophyton mentagrophytes, Microsporum gypsumoid and Epidermophyton flocculus, among which Trichophyton rubrum accounts for more than 60%, and has become the current hand, The main pathogenic bacteria of tinea pedis. Trichophyton rubrum (Trichophy ton rubrum) is a mitotic spore fungus and is the most common pathogenic dermatophyte in humans. It is distributed worldwide and is also the most common dermatophyte in my country. Trichophyton rubrum can cause skin and hair infections; and often causes chronic, refractory infecti...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K38/10A61K45/06A61P31/10A61P17/00A61K8/64A61Q17/00A61Q19/10
CPCA61K8/64A61K38/10A61K45/06A61K2800/78A61Q17/005A61Q19/10A61P17/00A61P31/10
Inventor 张海涛沈伟
Owner YANTAI HUAXIN BIOPHARM TECH CO LTD
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