Bone and bone tissue targeted exosome as well as preparation method and application thereof

An exosome, targeting technology, applied in chemical instruments and methods, biochemical equipment and methods, tissue culture, etc., can solve the problems of low abundance, volume-to-separation and difficulty in use, etc., and achieve increased expression and enhanced expression. The effect of specific aggregation and maintaining integrity

Pending Publication Date: 2020-11-03
赛瑞诚(苏州)生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the low abundance and extremely small size make it difficult to isolate and use these EVs

Method used

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  • Bone and bone tissue targeted exosome as well as preparation method and application thereof
  • Bone and bone tissue targeted exosome as well as preparation method and application thereof

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Embodiment Construction

[0024] The following will clearly and completely describe the technical solutions in the embodiments of the present invention with reference to the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only some, not all, embodiments of the present invention.

[0025] The present invention relates to a method of targeting extracellular vesicles using extracellular vesicles comprising a PTH component and obtained or obtainable therefrom by the methods disclosed herein. The specific technical scheme is as follows:

[0026] Construction, amplification and extraction of the fusion plasmid: insert the PTH component gene sequence and the Lamp2b gene CDS sequence into the downstream of the CMV promoter of the pcDNA3.1_hygro vector, transfer the connected vector into 293T cells for culture and amplification, and extract the transformed plasmid;

[0027] Preparation and extraction of PTH component chimeric exosomes: Transfect the m...

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Abstract

The invention relates to a bone and bone tissue targeted exosome and a preparation method and application thereof. The method adopts a molecule comprising a PTH (parathyroid hormone) domain and an extracellular vesicle obtained from or obtainable from the method disclosed herein, the method comprises the step of introducing a molecule comprising a parathyroid hormone-associated protein component (PTH component), into a fluid that possibly comprises the extracellular vesicle, wherein the PTH component comprises a PTH domain or an active fragment thereof.

Description

technical field [0001] The present disclosure relates to a method of targeting extracellular vesicles using molecules comprising a PTH (parathyroid hormone) domain and extracellular vesicles obtained or obtainable therefrom from the methods disclosed herein . Background technique [0002] Extracellular vesicles (also known as microvesicles or microparticles) are a carrier of cellular exocytosis and an important vehicle for intercellular communication. [0003] Extensive evidence shows that mammalian cells and their body fluids contain large numbers of extracellular vesicles, such as: healthy cells, stem cells and diseased cells, as well as plasma, saliva, urine, bile, synovial fluid, semen and breast milk. [0004] Studies have shown that extracellular vesicles can be used as a substitute for stem cells in therapy. However, the low abundance and extremely small size make it difficult to isolate and use these EVs. [0005] While exploring the mechanism of extracellular ves...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/10C12N15/85C12N15/62C12Q1/02A61K35/17A61P19/10
CPCC12N5/0686C07K14/635G01N33/5005A61K35/17A61P19/10C12N2510/02C07K2319/03
Inventor 修朝阳秦蕴豪
Owner 赛瑞诚(苏州)生物科技有限公司
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