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Dry chemical standard reagent sheet and preparation method thereof

A standard reagent, dry chemical technology, applied in the field of dry chemical standard reagent tablets and their preparation, can solve the problem that the color depth of the color card cannot completely cover the test signal range, the fluorescence calibration card is not suitable for dry chemical detection methods, and the color card absorption spectrum cannot be used. Complete matching and other problems, to achieve the effect of simple and fast preparation method, easy visual identification, and long-term stability

Pending Publication Date: 2020-11-03
中科嘉睿(天津)医疗科技发展有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Similar corrections have been used in fluorescent immunoassay analyzers. Patents 201510081035.7 and 201910004357.X disclose a fluorescent standard card and its preparation method. The standard card can calibrate the fluorescent immunoassay. However, the fluorescent calibration card is not suitable for dry Chemical detection method
[0003] Although the standard color cards on the market have a calibration function, the existing standard color cards mainly have the following problems: 1) The absorption spectrum of the color card cannot completely match the portable dry chemical analyzer; 2) The color depth of the same color card cannot be adjusted Fully covers the test signal range of the portable dry chemical analyzer; 3) The size and structure of the color card does not completely match the dry chemical analyzer, which has a great impact on the signal stability
Therefore, the existing color cards cannot meet the needs of calibrating dry chemical analyzers, and it is necessary to develop a new type of standard reagent sheet

Method used

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  • Dry chemical standard reagent sheet and preparation method thereof
  • Dry chemical standard reagent sheet and preparation method thereof
  • Dry chemical standard reagent sheet and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] A preparation method for a dry chemical standard reagent sheet, comprising the steps of:

[0046] (1) dissolving the dye in a solvent to form a dye solution;

[0047] The preparation method of the dye solution is as follows:

[0048] Dissolve 300 μL of blue oily color essence in 1 mL of acetone to form solution A, and mix solution A thoroughly to ensure that the blue oily color essence is completely dissolved and dispersed in acetone;

[0049]Dissolve 1.86g of gelatin, 0.861g of citric acid, and 0.334g of citric acid in 100mL of water to form solution B. Place solution B in a water bath at 50°C to fully dissolve the gelatin. After mixing, place it in a water bath at 37°C; Dye solutions C1-C7 with different concentrations in 7 were prepared according to the ratio shown in Table 1:

[0050] Table 1 embodiment 1 dye solution is prepared ingredient table

[0051]

[0052] (2) Apply the above-mentioned dye solutions of C1-C7 to the top of the washed and dried PET film ...

Embodiment 2

[0056] A preparation method for a dry chemical standard reagent sheet, comprising the steps of:

[0057] (1) dissolving the dye in a solvent to form a dye solution;

[0058] The preparation method of the dye solution is as follows:

[0059] Dissolve 0.025g of Coomassie Brilliant Blue in 1mL of water to form Solution A, and mix Solution A thoroughly to ensure that Coomassie Brilliant Blue is completely dissolved and dispersed;

[0060] Dissolve 2g of gelatin in 10mL of water to form solution B, place solution B in a water bath at 50°C to fully dissolve the gelatin, mix well and place in a water bath at 37°C; prepare 7 different concentrations according to the ratio shown in Table 2 below Dye solutions C1-C7:

[0061] Table 2 embodiment 2 dye solution preparation ingredient list

[0062] C1 C2 C3 C4 C5 C6 C7 Liquid A 1000μL 500μL 300μL 100μL 66.7μL 33.3μL 16.7μL water 0 μL 500μL 700μL 900μL 933.3μL 966.7μL 983.3μL Li...

Embodiment 3

[0066] Embodiment 3: Test the stability of standard reagent sheet

[0067] (1) Repeatability

[0068] Test 7 standard reagent sheets prepared by Example 1 with a dry chemistry analyzer, and the test result is the original voltage value (unit: mV) that is measured, and each standard reagent sheet measures 10 times, and the results are shown in the table below:

[0069] Table 3 Example 1 repeatability test result list

[0070]

[0071]

[0072] As can be seen from the above table, for the 7 standard reagent sheets prepared in Example 1, the same standard sheet was tested 10 times, and the CV was less than 1%. The test results of the standard sheet are stable and highly repeatable.

[0073] (2) Stability

[0074] The seven standard reagent sheets prepared in Example 1 were tested with a dry chemical analyzer for long-term monitoring, and the half-year test results are shown in Table 4 below.

[0075] Table 4 embodiment 1 stability test result data list

[0076]

[0...

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Abstract

The invention provides a dry chemical standard reagent piece which comprises a supporting layer, a dye layer, a reflective layer and an auxiliary layer which are sequentially arranged from bottom to top; the supporting layer is a transparent base film layer, the dye layer is of a layered structure formed by coating a dye solution on the supporting layer, and the reflective layer is a white polymeric film layer. The invention also provides a method for preparing the standard reagent sheet. According to the dry chemical standard reagent piece and the preparation method thereof, the prepared standard reagent piece is stable in performance, low in measurement variable coefficient and accurate in measurement result, can be completely matched with a dry chemical analyzer, and is suitable for calibration of the dry chemical analyzer.

Description

technical field [0001] The invention belongs to the field of dry chemical analyzer standard reagent sheet preparation, in particular relates to a dry chemical standard reagent sheet and a preparation method thereof. Background technique [0002] Dry chemical analyzers are widely used in the field of biomedical testing. By measuring the change in reflectance absorbance of the sample to be tested after the reaction of the chromogen on the reagent sheet, the content of the substance to be tested is analyzed by signal processing. Because the optical signal is very sensitive to the structure of the optical path, there are slight differences in the optical structure between the same batch of instruments and between different batches of instruments. This small difference will have a certain impact on the signal of the instrument, resulting in Signal deviation exists. At present, instrument calibration and quality control are widely used to improve the consistency of the test resul...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N1/28
CPCG01N1/28
Inventor 白鹏利钱庆章强李超刘勇马洁
Owner 中科嘉睿(天津)医疗科技发展有限公司
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