Fecal storage method
A preservation method and technology of feces, applied in the direction of biological material sampling method, chemical instrument and method, biochemical equipment and method, etc., can solve the problems of damage to the test product, large power consumption and cost of freezing, etc.
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Embodiment 1
[0253] Example 1: Sample stool drying test using granular silica gel
[0254] 1-1. Research on using Power Dry as drying conditions
[0255] Carry out the following experiments.
[0256] (1) Weigh the sample stool and put it into two 30mL test tubes (labcon).
[0257] (2) 1.27 g of Power Dry was put into a 30 mL test tube (labcon) so as to cover the sample stool, and left to stand at room temperature (25° C.). Under contact conditions, Power Dry and sample feces were packaged in the same closed space (30mL test tube).
[0258] (3) Open the package about 20 hours later, and observe the condition of the sample feces.
[0259] From the results, it is presumed that moisture was removed under the contact conditions because the sample stool became hard. The hardness of the sample feces requires considerable force even when trying to break it up with a metal scraper. Therefore, it was confirmed that the contact between the sample stool and the water-absorbing solid desiccant is ...
Embodiment 2
[0282] Example 2: Research on the influence of dry storage conditions of sample feces on the results of intestinal flora analysis
[0283] DNA was extracted from sample feces after drying and storage by various methods at room temperature, and the results of intestinal flora analysis were compared with the conventional method (Non-Patent Document 1).
[0284] Sampling conditions
[0285] Individual sample stools (amount of defecation at one time) were mixed so as to make the whole uniform, and distributed according to the following conditions, and each treatment was performed.
[0286] [Table 1]
[0287] List of sampling conditions
[0288]
[0289]
[0290] note
[0291] (1) "RNA stabilizer" was used at a ratio of 1 ml of RNAlaterTM stabilizing solution (manufactured by Thermo Fisher Scientific) to 100 mg of sample feces.
[0292] (2) The "preservation solution containing guanidine salt" was used at a ratio of 1 ml of the stable solution (guanidine thiocyanate solut...
Embodiment 3
[0299] Example 3: Research on the influence of dry storage conditions of sample feces on the analysis results of metabolites of intestinal flora
[0300]Under the conditions shown in Table 1, sample numbers 01 to 08, 13, and 14 extracted metabolites by the same method as described in International Publication No. 2017 / 043087. In addition, metabolites caused by sample No. 02 "freeze-drying after freezing at -80°C for 5 days" (conventional conditions) and sample No. 14 "silica gel_5 days" (conditions of the present invention) were compared The difference in concentration, and the concentration of various metabolites under these two conditions are shown in the scatter plot ( Figure 4 ).
[0301] As can be seen from the results, among the 513 metabolites to be detected, 103 were detected in "frozen at -80°C and then freeze-dried for 5 days" (traditional conditions), and 103 were detected in "silica gel-5 days" (the method of the present invention). Conditions) were detected in ...
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