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Method for extracting xanthophyll from algae and composition thereof

A technology for lutein and feed composition, which is applied in the directions of organic chemistry methods, chemical instruments and methods, animal feed, etc., can solve the problems of easy degradation of lutein, low efficiency, and high content of all-trans lutein, and improve the extraction rate. , improve efficiency, avoid the effect of configuration transformation

Active Publication Date: 2020-11-13
TIANJIN CHIATAI FEED TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method directly adds alkaline aqueous solution to the untreated marigold granules, which has the problems of low efficiency and easy degradation of lutein, and the highest product yield is about 82%, and the highest purity of all-trans lutein is 83%.
[0006] In summary, there is currently no method for extracting lutein using algae as a raw material with a high content of all-trans isomers of lutein in the product.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] The specific operation of the method for extracting lutein from Enteromorpha as raw material is as follows:

[0039] Take 100g Enteromorpha enteromorpha (the content of lutein was detected to be 2.23mg / g), wash it with clean water and remove impurities such as sediment, then crush it at 5°C, add 0.5L of the prepared compound enzyme solution (cellulase, semi A 3% aqueous solution with a mass ratio of cellulase and pectinase of 5:3:2, in which cellulase is 45IU / mg, hemicellulase is 45IU / mg, pectinase is 60IU / mg) , enzymatic hydrolysis at 50°C for 1 hour, centrifuged to take the supernatant, added 10 mL of 5% resveratrol ethanol solution, shaken gently, heated to 80°C and incubated for 10 minutes to inactivate the enzyme. Add 10 L of solvent obtained by mixing n-hexane and ethanol at a volume ratio of 1:9, heat to reflux, and extract for 3 hours to obtain a crude product solution. The obtained crude product solution was evaporated to dryness under reduced pressure at -0.0...

Embodiment 2

[0041] The specific operation of the method for extracting lutein from Enteromorpha as raw material is as follows:

[0042]Take 200g of Enteromorpha enteromorpha (the content of lutein has been tested to be 2.20mg / g), wash it with water and remove impurities such as sediment, break it at 5°C, add 1.2L of the prepared compound enzyme solution (cellulase, semi A 2% aqueous solution with a mass ratio of cellulase and pectinase of 5:3:3, in which cellulase is 45IU / mg, hemicellulase is 45IU / mg, pectinase is 60IU / mg) , enzymatic hydrolysis at 50°C for 2h, centrifuged to take the supernatant, added 20mL of 5% resveratrol ethanol solution, shaken gently, heated to 70°C and incubated for 10min to inactivate the enzyme. Add 20 L of solvent obtained by mixing tetrahydrofuran and ethanol at a volume ratio of 1:9, heat to reflux, and extract for 3 hours to obtain a crude product solution. 10 mL of 15% aqueous sodium hydroxide solution was added to the obtained crude product solution, evap...

Embodiment 3

[0044] The specific operation of the method for extracting lutein from Enteromorpha as raw material is as follows:

[0045] Take 100g Enteromorpha enteromorpha (the content of lutein has been tested to be 2.21mg / g), wash it with water and remove impurities such as sediment, crush it at 5°C, add 0.6L of the prepared compound enzyme solution (cellulase, semi A 2% aqueous solution with a mass ratio of cellulase and pectinase of 5:3:3, in which cellulase is 45IU / mg, hemicellulase is 45IU / mg, pectinase is 60IU / mg) , enzymatic hydrolysis at 50°C for 2h, centrifuged to take the supernatant, added 10mL of 5% tea polyphenol ethanol solution, shaken gently, heated to 80°C and kept for 10min to inactivate the enzyme. Add 10 L of solvent obtained by mixing tetrahydrofuran and ethanol at a volume ratio of 1:9, heat to reflux, and extract for 3 hours to obtain a crude product solution. The obtained crude product solution was evaporated to dryness under reduced pressure at -0.02MPa, rinsed ...

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PUM

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Abstract

The invention relates to a method for extracting xanthophyll from algae. The method comprises the following steps: pretreating algae, then carrying out enzymolysis to obtain an enzymolysis product, inactivating the obtained enzymolysis product, adding a solvent, carrying out reflux extraction to obtain a crude product solution, desolventizing the crude product solution, and carrying out post-treatment to obtain the product. The invention also discloses a feed composition containing the xanthophyll prepared by the method. The technical scheme provided by the invention has the advantages of highxanthophyll effective body content, high yield, stable effective body content and difficulty in configuration transformation, so that rich algae resources are effectively and reasonably utilized, andmeanwhile, the color and luster and the nutrition degree of livestock and poultry products can be effectively improved.

Description

technical field [0001] The invention belongs to the technical field of natural pigment extraction, and in particular relates to a method for extracting lutein from algae and a composition containing the extraction product. Background technique [0002] Lutein, also known as 3,3'-dihydroxy-α-carotene, has a molecular formula of C40H56O2. It is a natural carotenoid widely found in vegetables, flowers, fruits and some algae. It is often used as a natural carotenoid. Food pigments and food nutrients have obvious biological effects such as anti-oxidation, anti-aging and anti-mutation, and have a wide range of biological activities in many aspects, and are often used as color enhancers for livestock, poultry and animal tissues. in poultry feed. Because of its basic structural formula, two six-membered carbon rings are connected by a long chain of conjugated double bonds containing eighteen carbon atoms, with three chiral centers and up to eight stereoisomers, so it is difficult ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07C403/24C09B61/00C09B67/54A23K10/30A23K20/105A23K20/111A23K20/158A23L5/44
CPCA23K10/30A23K20/105A23K20/111A23K20/158A23K20/179C07B2200/09C07C403/24C09B61/00C09B67/0096C07C2601/16
Inventor 樊世明
Owner TIANJIN CHIATAI FEED TECH
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