Preparation method of Ceriporia lacerata spore liquid

A technology of tearing cereus and spore liquid, which is applied in the field of agricultural microorganisms, can solve the problems of inconvenient field spraying, affecting product quality, and forming ropes, etc., so as to facilitate field spraying and reduce storage and transportation costs , the effect of high product quality

Pending Publication Date: 2020-11-13
四川金珠生态农业科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the existing cereus tearing fungi are not easy to store and are inconvenient for field spraying, and the prior art is easy to agglomerate, ball or form ropes in the proce

Method used

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  • Preparation method of Ceriporia lacerata spore liquid
  • Preparation method of Ceriporia lacerata spore liquid
  • Preparation method of Ceriporia lacerata spore liquid

Examples

Experimental program
Comparison scheme
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preparation example Construction

[0044] A kind of preparation method of the cereus spore liquid of the present invention comprises the following steps:

[0045] Step 1, strain selection, select the strain of Ceriporia lacerata, which was preserved in the General Microbiology Center of China Committee for the Collection of Microbial Cultures on April 28, 2015, address: Beichen West Road 1, Chaoyang District, Beijing No. 3, Institute of Microbiology, Chinese Academy of Sciences, the preservation number is CGMCCNo.10485;

[0046] Step 2, slant seed culture:

[0047] After washing and slicing 200g of potatoes, boil them with an appropriate amount of deionized water for 30 minutes, filter with double-layer gauze, and take the filtrate for later use;

[0048] Weigh 20g of glucose and 20g of agar powder, dissolve in the above filtrate at the same time, heat and stir, after the agar powder is fully expanded and dissolved, use deionized water to make up to 1000ml, natural pH, and pack in Φ 25-34mm*290mm test tubes I...

example 1

[0111] The air flow rate of the 500L fermentation tank VVM1=1:1.2vvm, D1=900mm, D2=2200mm is calculated by substituting empirical formula ①,

[0112] VVM2=VVM1×(D1 / D2) 2 / 3

[0113] =1.2×(900 / 2200) 2 / 3

[0114] =0.66

[0115] ≈0.7vvm.

[0116] Therefore, the air flow rate in the fermentation tank of the present invention is about 1:0.7, which is more suitable.

[0117] 2. Calculation of the speed of the fermentation tank

[0118] Empirical formula ②: N 2 =N 1 *(VVM 1 *V 1 *D 2 2 / VVM 2 *V2*D 1 2 ) 0.23 *(d 1 / d 2 ) 0.54

[0119] N1----speed of 500L fermenter VVM1----air flow of 500L fermenter

[0120] V1----filling volume of 500L fermenter D1----diameter of 500L fermenter

[0121] d1----the paddle diameter of the 500L fermentation tank

[0122] N2----rotational speed of 20t fermenter VVM1----air flow of 20tL fermenter

[0123] V1----the charging volume of the 20t fermenter D1----the diameter of the 20t fermenter

[0124] d1----the paddle diameter of the ...

example 2

[0126] Air flow VVM1=1:1.2vvm of 500L fermentation tank, tank diameter D1=900mm, loading capacity V1=350L, speed N1=150rpm, paddle diameter d1=250mm;

[0127] 20t fermentation tank VVM1=1:0.7vvm, tank diameter D1=2200mm, loading V1=12000L, paddle diameter d2=670mm;

[0128] Substituting into empirical formula ② to calculate,

[0129] N 2 =150rpm×(1.22vvm×350L×2200 2 / 0.7vvm×12000L×900 2 ) 0.23 ×(250 / 670) 0.54 =150rpm×0.299 0.23 ×0.37 0.54

[0130] =67rpm

[0131] ≈70rpm

[0132] In actual use, it is necessary to judge whether the fermentation conditions are suitable according to the conditions of the fermentation tank site such as foam conditions, liquid level and tumbling conditions, etc.; based on 70rpm, the speed can be increased to 140 according to the situation on site. Or reduce to 60, so that the fermentor speed range is 60-140rpm.

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Abstract

The invention relates to the technical field of agricultural microorganisms, and discloses a preparation method of a Ceriporia lacerata spore liquid, which comprises the following steps: slant seed culture, shake flask seed culture, seed tank culture, fermentation tank culture, fermentation broth pretreatment, and sealed storage to obtain the Ceriporia lacerata spore liquid. Through slant seed culture, rich aerial hyphae are obtained; through shake flask seed culture, stretched and slender hyphae without agglomeration and caking phenomena are obtained; the concentration of thalli is increasedthrough seed tank culture; continuously increasing the concentration of mycelia through culture in the fermentation tank; after fermentation is completed, the fermentation liquid is pretreated to promote release of enveloped spores to obtain pretreated fermentation liquid, and then the pretreated fermentation liquid is sub-packaged, sealed and stored to obtain the Ceriporia lacerata spore liquid which is convenient to store and more beneficial to field spraying and use. In addition, in the hypha culture process, the appearance is free of caking, the phenomenon of balling or filamentous formation is reduced, and the product quality is high.

Description

technical field [0001] The invention belongs to the technical field of agricultural microorganisms, and in particular relates to a preparation method of a spore liquid of the cereus cerevisiae. Background technique [0002] The use of chemical fertilizers and pesticides in my country ranks first in the world. The high input of chemical fertilizers and pesticides has brought about a series of environmental, ecological and food safety problems. In modern intensive agricultural production, a large number of frequent application of pesticides is required to prevent and control crop diseases. However, the long-term and large-scale application of pesticides has caused a series of production, environmental and health problems, such as enhanced drug resistance of bacteria, reduced drug efficacy, increased dosage, increased pesticide residues in the environment and agricultural products, affecting food safety, endangering human health, etc. . [0003] Fungal diseases account for 70...

Claims

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Application Information

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IPC IPC(8): C12N1/14C12N3/00C12R1/645
CPCC12N1/14C12N3/00
Inventor 不公告发明人
Owner 四川金珠生态农业科技有限公司
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