Novel preserving fluid for microbial nucleic acid in sputum

A technology of microorganisms and preservation solutions, which is applied in the field of molecular biology, can solve the problems of poor practicability of sputum samples, insufficient storage time, and easy breeding of microorganisms, so as to avoid degradation, good biosolubility, and good bactericidal and antibacterial properties Effect

Pending Publication Date: 2020-11-13
广州源古纪科技有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, some patents have disclosed methods for protecting nucleic acids. However, the technologies involved in the existing patents are not practical for sputum samples, and most of them still have the following prob...

Method used

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  • Novel preserving fluid for microbial nucleic acid in sputum
  • Novel preserving fluid for microbial nucleic acid in sputum
  • Novel preserving fluid for microbial nucleic acid in sputum

Examples

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Embodiment

[0036] 210g guanidine isothiocyanate is dissolved in the sterilized water of equal quality, preparation mass concentration is the guanidine isothiocyanate aqueous solution of 50%, and 30g 8-hydroxyquinoline is dissolved in 90g isopropanol and preparation mass concentration is 25% 8-hydroxyquinoline-isopropanol solution, mixing guanidine isothiocyanate aqueous solution and 8-hydroxyquinoline-isopropanol solution to obtain nuclease inhibitor.

[0037] Dissolve 35 g of Tris in 105 g of sterilized water to prepare a 25% Tris solution as a pH stabilizer.

[0038] Dissolve 10 g of disodium ethylenediamine edetate in 30 g of sterilized water to prepare a 25% disodium edetate solution as a chelating agent.

[0039] Mix the prepared nuclease inhibitor, pH stabilizer, and chelating agent evenly, then add 1g of mucolytic agent N-acetyl-L-cysteine ​​and 4g of sodium lauryl sarcosine, stir well, and then add 100g glycerol and 60g isopropanol, finally add 115g sterilized water, stir to obt...

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Abstract

The invention discloses a novel preserving fluid for microbial nucleic acid in sputum. The preserving fluid comprises the following components in percentage by mass: 15-30% of nuclease inhibitor, 1.5-5% of a pH value stabilizer; 0.8%-1.5% of a chelating agent; 0.05%-0.15% of a mucus dissolving agent; 0.1 to 0.5 percent of sodium dodecyl sarcosinate; 10 to 20 percent of isopropanol; 2 to 15 percentof glycerol; the balance of sterilized water. The novel preserving fluid for microbial nucleic acid in sputum can rapidly dissolve and stabilize sputum samples, effectively inhibit nuclease activityand prevent cell lysis, has good sample antibacterial property, long preservation time, relatively simple components, capability of keeping nucleic acid at room temperature, and suitableness for wideclinical application.

Description

technical field [0001] The invention relates to the technical field of molecular biology, in particular to a novel preservation solution for microbial nucleic acids in sputum. Background technique [0002] Phlegm is a liquid secreted by stimulation of the respiratory tract, also called sputum, and its components include mucus, foreign bodies, pathogenic microorganisms, various inflammatory cells, necrotic and exfoliated mucosal epithelial cells, etc. Currently, sputum samples are mainly used for sputum cytology detection and respiratory pathogen detection in clinical practice. Among them, identifying the type of pathogen by detecting the nucleic acid of the pathogen in the sputum sample is currently the most accurate and rapid diagnostic method for respiratory pathogens. However, in practice, nucleic acids (especially RNA) in sputum samples are extremely unstable and are usually degraded within hours under greenhouse conditions. External physical factors that cause nucleic ...

Claims

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Application Information

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IPC IPC(8): C12Q1/6806
CPCC12Q1/6806C12Q2527/127C12Q2527/125
Inventor 段志峰
Owner 广州源古纪科技有限公司
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