Application of 2-substituted anthraquinone derivative to preparation of medicine for treatment of candida albicans infection

A technology of anthraquinone derivatives and Candida albicans, which is applied in the direction of chemicals, applications, and biocides for biological control, and can solve the problems of no relevant reports on impact

Active Publication Date: 2020-11-20
CHINA PHARM UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0010] At present, there is no relevant report on the effect of 2-substituted anthraquinone derivatives such as 2-ethylanthraquinone on Candida albicans biofilm

Method used

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  • Application of 2-substituted anthraquinone derivative to preparation of medicine for treatment of candida albicans infection
  • Application of 2-substituted anthraquinone derivative to preparation of medicine for treatment of candida albicans infection
  • Application of 2-substituted anthraquinone derivative to preparation of medicine for treatment of candida albicans infection

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Example 1: 2-ethylanthraquinone has the effect of inhibiting the formation of Candida albicans biofilm.

[0043] Experimental method: crystal violet staining method.

[0044] Glycerol tubes of Candida albicans were inoculated into YPD liquid medium, and cultured on a shaker at 150 rpm at 37°C for 24 hours. Dip the bacterial liquid and inoculate it on the LB solid plate culture dish. After inverting for 24 hours at 37°C, pick a single colony with high activity and inoculate it in the YPD liquid medium, and culture it to the logarithmic growth phase at 37°C and 150rpm shaker. Collect Candida albicans cells, dilute with RPMI1640 medium (containing 10% fetal bovine serum FBS) to inoculate the final concentration of 96-well plate to be 10 5 CFU / mL, ie OD 600 = 0.1.

[0045] 2-Ethylanthraquinone was dissolved in dimethyl sulfoxide (DMSO) to prepare a stock solution of 10 mg / mL, and diluted with RPMI 1640 medium (containing 10% FBS) to add to a 96-well plate at a final conc...

Embodiment 2

[0052] Example 2: 2-ethylanthraquinone has the effect of inhibiting the metabolic activity of Candida albicans biofilm cells.

[0053] Experimental method: XTT reduction method.

[0054] When different concentrations of 2-ethylanthraquinone existed or not, Candida albicans was allowed to form a biofilm in a 96-well plate, and the culture method was the same as in Example 1. Prepare 1mg / mL XTT solution (dissolved in Ringer's solution) and 68.875mg / L menadione solution (dissolved in acetone), mix XTT solution with menadione-acetone solution at 5:1 (v / v) . Each well of the 96-well plate was washed twice with sterile water, 100 μL of XTT-menadione mixed solution was added, incubated at 37°C for 2 hours in the dark, and the absorbance at 490 nm was measured.

[0055] The calculation method of biofilm cell metabolism inhibition rate is as follows:

[0056] Biofilm cell metabolism inhibition rate=(1-(experimental group absorbance value-zero hole) / (control group absorbance value-ze...

Embodiment 3

[0059] Example 3: 2-ethylanthraquinone has the effect of inhibiting the mycelial growth of Candida albicans.

[0060] Experimental method: The hyphal growth of Candida albicans was observed by electron microscope.

[0061] Cultivate Candida albicans in YPD liquid medium to logarithmic growth phase, collect Candida albicans cells, wash once with sterile water, and inoculate again into RPMI 1640 medium containing different concentrations of 2-ethylanthraquinone (+10 %FBS), cultured at 37° C., 150 rpm for 24 h, and the blank control group was without 2-ethylanthraquinone. Collect the culture medium containing Candida albicans, put 20 μL on a glass slide, and observe the hyphal growth of Candida albicans with an ordinary electron microscope.

[0062] According to the results, the inhibitory effect of 2-ethylanthraquinone on the mycelial growth of Candida albicans is as follows: Figure 5 shown. Compared with the blank control group, when the concentration of 2-ethylanthraquinon...

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Abstract

The invention belongs to the technical field of medicines, and relates to an application of a 2-substituted anthraquinone derivative in treatment of candida albicans infection. Candida albicans can cause infection of the tissues and organs of a host or any part of the skin surface and can be adhered to the surfaces of various medical devices, and many diseases caused by the candida albicans are related to biofilm formation. Experiments prove that 2-ethylanthraquinone, 2-methylanthraquinone, 2-tert-butylanthraquinone and 2-hydroxyanthraquinone have an inhibiting effect on biofilm formation of the candida albicans and can reduce the metabolic activity of biofilm cells, and the 2-ethylanthraquinone also has an effect of inhibiting mycelia formation of the candida albicans. It is proved that the 2-substituted anthraquinone derivative has a good application prospect in preparation of a medicine for treatment of the candida albicans infection.

Description

technical field [0001] The invention belongs to the field of new applications of anthraquinone compounds, and specifically relates to the application of a class of anthraquinone compounds - 2-substituted anthraquinone derivatives in the treatment of Candida albicans infection. The 2-substituted anthraquinone derivatives can be used to prepare Candida albicans Bacterial biofilm formation inhibitors or cleaning agents for medical instruments. Background technique [0002] Biofilm is a form of extracellular polymer matrix that is produced by microbial communities for self-protection and can adhere to the surface of biological or non-biological materials to wrap cell communities. It is formed by bacteria and fungi in order to adapt to the living environment during their growth. A mode of survival corresponding to planktonic cells. Candida albicans is one fungus that can form biofilms. [0003] The biofilm formation process of Candida albicans is mainly divided into four steps:...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/122A61P31/10A01N35/06C11D3/48C11D3/20A01P3/00
CPCA61K31/122A61P31/10A01N35/06C11D3/48C11D3/2072
Inventor 陆园园宋媛媛
Owner CHINA PHARM UNIV
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