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Method for preparing epigallocatechin gallate and application of epigallocatechin gallate in anti-sugar products

A technology of epigallocatechin and gallate, applied in the field of chemical purification, can solve the problems of collagen and elastin not functioning normally, skin sagging, etc., and achieves improved anti-sugar effect, significant effect, improved yield and The effect of purity

Active Publication Date: 2020-11-20
BEIJING AOTE SHUER HEALTH PROD DEV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Collagen and elastin after glycation will not function properly, resulting in gradual sagging of the skin

Method used

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  • Method for preparing epigallocatechin gallate and application of epigallocatechin gallate in anti-sugar products
  • Method for preparing epigallocatechin gallate and application of epigallocatechin gallate in anti-sugar products
  • Method for preparing epigallocatechin gallate and application of epigallocatechin gallate in anti-sugar products

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0056] A method for preparing epigallocatechin gallate, comprising the steps of:

[0057] 1) Dissolving the crude tea polyphenols in water, the mass volume ratio of the crude tea polyphenols to water is 1:30g / mL, the dissolution temperature is 30°C, the dissolution time is 1h, and filtered to obtain the tea polyphenols solution;

[0058] 2) Concentrate the tea polyphenol solution to 2 times the amount of the crude tea polyphenols, extract with solvent A that is 4 times the amount of the crude tea polyphenols, collect the organic layer, concentrate and dry, and dissolve it in water that is 4 times the amount of the crude tea polyphenols , to obtain a mixed solution;

[0059] 3) Add the mixed solution into the HP-20 chromatography column, use solvent B as the mobile phase to elute, the elution flow rate is 1BV / h, the elution volume is 4BV, and the eluate is collected;

[0060] 4) Use HPD450 resin macroporous adsorption resin to adsorb the eluent, and use 45% ethanol solution fo...

Embodiment 2

[0069] A method for preparing epigallocatechin gallate, comprising the steps of:

[0070] 1) Dissolve the crude tea polyphenols in water, the mass volume ratio of the crude tea polyphenols to water is 1:80g / mL, the dissolution temperature is 70°C, the dissolution time is 2h, and filter to obtain the tea polyphenols solution;

[0071] 2) Concentrate the tea polyphenol solution to 10 times the amount of the crude tea polyphenols, extract with solvent A 10 times the amount of the crude tea polyphenols, collect the organic layer, concentrate and dry, and dissolve it in water 10 times the amount of the crude tea polyphenols , to obtain a mixed solution;

[0072] 3) Add the mixed solution to the RS-24 chromatography column, and use solvent B as the mobile phase for elution, the elution flow rate is 2BV / h, the elution volume is 6BV, and the eluate is collected;

[0073] 4) Use D180 macroporous adsorption resin to adsorb the eluent, and use 60% ethanol solution to elute, the elution ...

Embodiment 3

[0082] A method for preparing epigallocatechin gallate, comprising the steps of:

[0083] 1) Dissolve the crude tea polyphenols in water, the mass volume ratio of the crude tea polyphenols to water is 1:55g / mL, the dissolution temperature is 50°C, the dissolution time is 1.5h, and filter to obtain the tea polyphenols solution;

[0084] 2) Concentrate the tea polyphenol solution to 6 times the amount of crude tea polyphenols, extract with solvent A that is 7 times the amount of crude tea polyphenols, collect the organic layer, concentrate and dry, and dissolve with water that is 7 times the amount of crude tea polyphenols , to obtain a mixed solution;

[0085] 3) Add the mixed solution into the HPD-600 chromatography column, and use solvent B as the mobile phase for elution, the elution flow rate is 1.5BV / h, the elution volume is 5BV, and the eluate is collected;

[0086] 4) Use AB-8 macroporous adsorption resin to adsorb the eluent, and use 55% ethanol solution for elution. T...

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Abstract

The invention relates to the technical field of chemical purification, in particular to a method for preparing epigallocatechin gallate and application of epigallocatechin gallate in anti-sugar products. The method comprises the following steps: 1) dissolving a tea polyphenol crude product with water, and filtering to obtain a tea polyphenol solution; 2) concentrating the tea polyphenol solution,extracting with a solvent A, collecting an organic layer, concentrating, drying, and dissolving with water to obtain a mixed solution; (3) adding the mixed solution into a chromatographic column, eluting by taking a solvent B as a mobile phase, and collecting eluent, and (4) adsorbing the eluent by utilizing macroporous adsorption resin, eluting by adopting a solvent C, collecting the eluent, concentrating under reduced pressure, and freeze-drying, so as to obtain the product. The method has the advantages of high yield and purity, simple preparation process and low cost.

Description

technical field [0001] The invention relates to the technical field of chemical purification, in particular to a method for preparing epigallocatechin gallate and its application in anti-sugar products. Background technique [0002] Tea polyphenols (Green Tea Polyphenols, abbreviated as GTP), is one of the main active ingredients in tea. It is a compound of polyhydric phenolic compounds in tea. Theanin and its derivatives are the main chemical components in tea with health care functions. Tea polyphenols have various physiological activities such as anti-oxidation, anti-radiation, anti-aging, lowering blood fat, lowering blood sugar, and inhibiting bacteria and enzymes. [0003] Catechin substances in tea polyphenols account for about 60-80%, and the content of epigallocatechin gallate (abbreviated as EGCG) is the highest, accounting for about 50-60% of catechins. EGCG has many active characteristics such as antibacterial, antiviral, antioxidative, antiaging, repairing ski...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D311/62A61K31/353A61K31/7004A61K36/70A61K36/48A61K36/064A61P17/00A61P3/10A61P9/00A61P13/12A61P37/04
CPCA61K31/353A61K31/7004A61K36/064A61K36/48A61K36/70A61P3/10A61P9/00A61P13/12A61P17/00A61P37/04C07D311/62A61K2300/00
Inventor 赵一弘雷雨
Owner BEIJING AOTE SHUER HEALTH PROD DEV