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Fructosamine determination kit

A kit and fructosamine technology, applied in the field of fructosamine determination kits, can solve the problems of affecting the stability and accuracy of detection reagents, affecting the accuracy of fructosamine, poor solubility of NBT, etc., and achieving enhanced solubility and reagent stability. , Improved precision and linear range, excellent linearity and precision

Active Publication Date: 2020-11-20
WUHAN LIFE ORIGIN BIOTECH LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, during the detection process, there will be interference from reducing substances such as ascorbic acid, bilirubin, and cholesterol in serum samples, which will affect the accuracy of fructosamine detection results, and it is difficult to effectively solve all interference problems only by relying on a strong alkaline environment; secondly The solubility of NBT is poor, so the substrate will precipitate out during the long-term storage of the reagent, which affects the stability and detection accuracy of the detection reagent.

Method used

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] The optimization of embodiment 1 R1 reagent

[0023] This embodiment mainly optimizes the R1 reagent, wherein the R1 reagent includes: 250mmol Na 2 CO 3 -NaHCO 3 Buffer solution, 5g / L NaCl, 1g / L sodium azide and Emulgen series surfactants (all are Kao products). Among them, the specific material selection and concentration of Emulegen series surfactants are mainly optimized, as shown in the following table:

[0024] group Emulegen series surfactants concentration 1 Emulgen A90: Emulgen B66 = 1:1 1g / L 2 Emulgen A90 1g / L 3 Emulgen B66 1g / L 4 Emulgen A90: Emulgen 430 = 1:1 1g / L 5 Emulgen B66: Emulgen A60 = 1:1 1g / L 6 Emulgen A500: Emulgen709 = 1:1 1g / L 7 Emulgen A90: Emulgen B66 = 1:1 0.5g / L 8 Emulgen A90: Emulgen B66 = 1:1 2g / L 9 Emulgen A90: Emulgen B66 = 1:1 2.5g / L 10 Surfactant free 0g / L

[0025] The R2 reagent is a conventional reagent, specifically including: pH7.5 100...

Embodiment 2R2

[0031] The optimization of embodiment 2R2 reagent

[0032] R1 reagent is in the present embodiment: 250mmol Na 2 CO 3 -NaHCO 3 Buffer, 5g / L NaCl, 1g / L sodium azide and 1g / L mixture of Emulgen A90 and Emulgen B66 in equal volume ratio. This embodiment mainly optimizes the R2 reagent, as shown in the following table:

[0033] group Citric acid-sodium citrate buffer isopropylamine benzene sulfonate NBT Sodium azide 1 pH 4.5, 15mmol / L 1g / L 2g / L 2g / L 2 pH7.0, 15mmol / L 1g / L 2g / L 2g / L 3 pH3.0, 15mmol / L 1g / L 2g / L 2g / L 4 pH 4.5, 15mmol / L 0.3g / L 2g / L 2g / L 5 pH 4.5, 15mmol / L 2.5g / L 2g / L 2g / L 6 pH 4.0, 15mmol / L 2g / L 2g / L 2g / L 7 pH 5.0, 15mmol / L 0.5g / L 2g / L 2g / L

[0034] Perform performance evaluation on the above 7 groups of kits, including:

[0035] (1) Blank absorbance: the absorbance value of the reagent at a wavelength of 546nm.

[0036] (2) Accuracy: Detect the quality contro...

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Abstract

The invention discloses a fructosamine determination kit. The kit comprises a reagent R1 and a reagent R2, wherein the pH value of the reagent R1 is 10.0-10.8, and the reagent R1 comprises 200-500mmol / L of an alkaline buffer solution, 1-10g / L of a metal salt, 0.5-2g / L of a mixture of Emulgen A90 and Emulgen B66 with the same volume ratio, and 0.5-2g / L of a preservative; and the pH value of the reagent R2 is 4.0-5.0, and the reagent R2 comprises 5-25mmol / L of an acidic buffer solution, 0.5-2g / L of isopropylamine alkylbenzene sulfonate, 1-5g / L of NBT and 0.5-2g / L of a preservative. According tothe invention, the mixture of Emulgen A90 and Emulgen B66 with the same volume ratio is specially selected from the reagent R1 and is matched with a strong alkaline environment, so that interferents in a serum sample are effectively removed; in addition, an acid environment and isopropylamine alkylbenzene sulfonate are matched for use in the reagent R2, the concentration of the buffer solution isproperly reduced to enhance the solubility of NBT and the stability of the reagent, and meanwhile, the precision and linear range of the reagent are improved, so that the fructosamine determination kit with remarkably enhanced anti-interference capability and stability and more excellent reagent linearity and precision is obtained.

Description

technical field [0001] The invention belongs to the technical field of clinical chemical detection, and in particular relates to a fructosamine determination kit. Background technique [0002] Fructosamine is a substance formed by protein in plasma during non-enzymatic saccharification of glucose. Its concentration is positively correlated with blood sugar level and relatively stable, and the determination of fructosamine is not affected by blood sugar. Since the half-life of plasma protein is 17-20 days, fructosamine can reflect the average blood sugar level of diabetic patients within 1-3 weeks before testing. To a certain extent, it makes up for the deficiency that glycosylated hemoglobin cannot reflect the change of blood sugar concentration in a short period of time, and is a research index for the long-term blood sugar control level of clinical diabetic patients. [0003] There are many methods for the determination of fructosamine, mainly chemical and chromatography....

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/66
CPCG01N33/66
Inventor 吴年芬龚婷梁艳张雪娇舒芹
Owner WUHAN LIFE ORIGIN BIOTECH LTD
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