Effect of apigenin in preparation of medicine for promoting survival of ischemic random flap

A kind of apigenin and ultra-long technology, applied in the direction of drug combination, pharmaceutical formula, organic active ingredients, etc., can solve the problem of unknown skin flap survival and treatment effect, reduce skin flap cell damage, increase blood vessel density and blood flow, The effect of improving the survival rate

Inactive Publication Date: 2020-11-24
THE SECOND HOSPITAL AFFILIATED TO WENZHOU MEDICAL COLLEGE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

According to existing reports, apigenin can promote the expression of vascular endothelial growth factor (VEGF) in the perfusion injury of focal cerebral ischemia, and promote angiogenesis after ischemia, but the therapeutic effect on flap survival is unknown

Method used

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  • Effect of apigenin in preparation of medicine for promoting survival of ischemic random flap
  • Effect of apigenin in preparation of medicine for promoting survival of ischemic random flap
  • Effect of apigenin in preparation of medicine for promoting survival of ischemic random flap

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Example 1: Detection of skin flap survival area ratio and skin flap survival status

[0032] From 1 to 7 days after operation, the color, texture, tissue elasticity and hair growth or necrosis of rat skin flaps were observed daily with naked eyes and recorded. On the 7th day, under anesthesia, the rats in each group accurately measured the survival and necrosis area of ​​the skin flap with transparent paper, cut it into two parts, the living part and the dead part, and weighed them with electronic scales. Calculate the percentage of flap survival area [flap survival area transparent paper mass ÷ total flap surface area transparent paper mass × 100%].

[0033] Flap necrosis criteria: the color of the flap is black, the tissue retracts, the elasticity is poor, the texture is hard, and the cut tissue does not bleed. The pathological manifestations were tissue disintegration, nucleus disappearance, extensive infiltration of inflammatory cells, and focal hemorrhage.

[003...

Embodiment 2

[0036] Example 2: detection of skin flap neovascularization

[0037] Using laser Doppler blood flowmeter to detect the neovascularization of skin flap is a device that uses the principle of laser Doppler to monitor the blood perfusion of microcirculation in animals or human tissues. The laboratory of this subject has the instrument and supporting operating software to collect and analyze blood flow signals. Seven days after the operation, the blood flow at the back flap of the rats was measured, and reports were made in the form of general report, percentage report, PORH report, and frequency report, and the effective blood flow signal at the neovascularization site was observed.

[0038] Such as Figure 5As shown, after 7 days, the laser Doppler flowmeter showed that the blood flow of the skin flap in the apigenin group was significantly more than that in the corn oil control group, and the blood perfusion of the middle flap in the high-dose apigenin group was (345.65±71.87)...

Embodiment 3

[0041] Embodiment 3: Histological detection

[0042] Seven days after surgery, samples were taken at the midpoint of the flap. The samples were fixed with 10% formaldehyde, embedded in paraffin, and stained with HE. The thickness of the granulation tissue layer, the structural changes of capillaries, tissue edema, necrosis, and inflammatory cell infiltration were observed under a light microscope 7 days after the operation .

[0043] The result is as Figure 7 As shown, fibroblast proliferation, thinner granulation tissue, less tissue edema, and less inflammatory cell infiltration were seen in the apigenin experimental group, and the lower dose of the high-dose group consisted of more fibroblasts, thinner granulation tissue, less tissue edema, and less inflammatory cells Less infiltration. In the control group, fibroblast proliferation was less, tissue edema was obvious, and inflammatory cell infiltration was serious.

[0044] In the high-dose apigenin group, the microvess...

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Abstract

The invention relates to an effect of apigenin in preparation of a medicine for promoting survival of ischemic random flap. An oral administration mode is adopted in the process of promoting the survival of the ischemic random flap; a use method is as follows: the daily oral dosage is 20 mg / kg or 50 mg / kg, and 5-7 days are taken as a course of treatment; and a conclusion is drawn through a large number of animal experiments, and by using the apigenin, the survival area ratio of the super-long random flap can be obviously increased, inflammation is reduced, the oxidation resistance is improved,the vascular density is greatly increased, and the survival of the ischemic random flap can be well promoted.

Description

technical field [0001] The invention relates to a new application of apigenin, in particular to the role of apigenin in promoting the survival of ischemic ultra-long random skin flaps. Background technique [0002] In recent years, with the continuous improvement of my country's economic level, industries such as industry and energy have shown a trend of rapid development, and the resulting cases of large-area trauma and tissue defects related to occupations and traffic behaviors are also increasing. This type of trauma usually leaves different degrees of deformity and dysfunction after repair, especially trauma deformity of the hands and face. As patients' requirements for perfect appearance and functional recovery are increasing day by day, the repair technology and treatment methods for wound problems have become an important consideration in medicine. Clinically, as the most basic and most commonly used surgical method for wound repair, random flaps are widely used clin...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/352A61P9/10
CPCA61K31/352A61P9/10
Inventor 林丁盛马心怡何纪兵方妙杰李文杰刘莹莹
Owner THE SECOND HOSPITAL AFFILIATED TO WENZHOU MEDICAL COLLEGE
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