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Method for extracting compound axillarin from xanthium italicum and application of compound axillarin

A technology of methoxy stevia, Italian cocklebur, applied in the field of herbicides, can solve problems such as difficult to effectively remove, agriculture, forestry and animal husbandry, and ecosystem impact and harm, and achieves environmental protection, easy to apply in large quantities, and does not pollute the environment. Effect

Active Publication Date: 2020-11-24
LINYI UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to its strong ecological adaptability and large biomass, the alien invasive cocklebur will compete with native crops for nutrients and space. At the same time, its fruit has hooked thorns, which are difficult to effectively remove, causing serious impact and harm to the agriculture, forestry, animal husbandry and ecosystem of the invaded area. , see literature 10-11

Method used

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  • Method for extracting compound axillarin from xanthium italicum and application of compound axillarin
  • Method for extracting compound axillarin from xanthium italicum and application of compound axillarin
  • Method for extracting compound axillarin from xanthium italicum and application of compound axillarin

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Extraction of methoxyshoujuin:

[0039] a. Collect the Italian cocklebur plants in the mature stage, separate the fruit from other parts, dry and pulverize for subsequent use;

[0040] b. Soak the cocklebur fruit crushed in step a with 5 times the volume of 95% ethanol at room temperature for 7 days, filter the soaking solution to remove the residue, and then steam the filtrate with a rotary evaporator until it has no alcohol smell , get the extract;

[0041] c. Stir the extract obtained in step b with distilled water, add an equal volume of petroleum ether to degrease, repeat 3 times, collect the supernatant, and obtain a suspension after degreasing;

[0042] d, degreasing the suspension in step c, then extracting 3 times with an equal volume of chloroform, combining the extracts, and concentrating with a rotary evaporator to obtain an extract;

[0043] e, the extract obtained in step d is chromatographed with a 100-200 mesh silica gel column, and the chloroform:meth...

Embodiment 2

[0046] The use of methoxyshoujuin in the preparation of herbicides:

[0047] The methoxystuchinol obtained in Example 1 was first formulated with 0.5% acetone to make 1000 μg / ml, and then diluted with distilled water to a concentration of 10 μg / ml, 50 μg / ml or 200 μg / ml. ml, 50 μg / ml, 200 μg / ml and 1000 μg / ml of methoxystuchinin solutions were sequentially added to each petri dish with a diameter of 9 cm, and a layer of filter paper was spread on each petri dish, and each petri dish was separately Add 20 plump seeds of alfalfa, pennisetum, bluegrass and retroflex amaranthus, do 3 repetitions for each petri dish, seal it with a parafilm, put it in an incubator at a temperature of 25°C, light for 16 hours, and dark for 8 hours. After cultivating for 5 days, measure the seedling height and root length of the seedlings in each petri dish, and use distilled water containing 0.5% acetone as a contrast;

[0048] Data statistics method: The root length and seedling height data of eac...

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Abstract

The invention discloses a method for extracting a compound axillarin from xanthium italicum and an application of the compound axillarin. The method comprises the following steps of crushing fruits ofthe xanthium italicum in a mature period, soaking the crushed fruits in ethanol for crude extraction, performing degreasing with petroleum ether, performing extracting with trichloromethane, performing purifying with a silica gel column, and performing purifying with a Sephadex LH-20 column to obtain the axillarin. The obtained axillarin is used as a herbicide for trial use in three tested plants, namely monocotyledonous plants such as bluegrass and pennisetum alopecuroides, and dicotyledonous plants such as amaranthus retroflexus; results show that seedling growth of the monocotyledonous plants such as the bluegrass and the pennisetum alopecuroides and the dicotyledonous plants such as the amaranthus retroflexus is significantly inhibited; and the root length inhibition rate of the amaranthus retroflexus reaches 97%, the root length inhibition rate of the pennisetum alopecuroides is 52%, and the root length inhibition rate of the bluegrass is 43%. The axillarin is a plant source natural component, can be rapidly decomposed in nature and does not pollute the environment. The axillarin can be prepared into herbicides with different concentrations, can be independently used or can be mixed with other pesticides for use before seedling or in a seedling stage, is widely applied to development of various herbicides, and has potential commercial application value.

Description

technical field [0001] The invention relates to a preparation method for extracting flavonoids from plants and the technical field of application to herbicides, in particular to a preparation method for extracting the compound methoxyshouju from the harmful plant Cocklebur annuus and its use as a herbicide Applications. Background technique [0002] With the development of modern agriculture, more than 80% of the weed control in agriculture, forestry and animal husbandry is favored by chemical herbicides. Its labor-saving, time-saving, labor-saving, low-cost and easy-to-operate advantages have brought huge economic development to agriculture. benefit. However, with the mass production and widespread use of chemical herbicides, they often cause serious harm to the natural environment and are killers of the ecological environment. At the same time, a variety of synthetic chemical herbicides are likely to remain in nature for a long time due to the lack of corresponding micro...

Claims

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Application Information

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IPC IPC(8): C07D311/30C07D311/40A01N43/16A01P13/00
CPCC07D311/30C07D311/40A01N43/16Y02A50/30
Inventor 张弛黄岭邵华
Owner LINYI UNIVERSITY
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