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Method for screening short-cut nitrifying bacteria by using low-intensity ultrasonic waves and application thereof

A short-range nitrification and ultrasonic technology, which is applied in the direction of chemical instruments and methods, biochemical equipment and methods, bacteria, etc., can solve the problems of short-range nitrification system instability, and achieve the promotion of biological denitrification efficiency, reduction of environmental pollution, and energy consumption low effect

Pending Publication Date: 2020-12-04
JIANGXI UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the application number: 2019103479720, the applicant used an ultrasonic generator to ultrasonically treat the concentrated activated sludge in the SBR reactor, which overcomes the problem that the short-range nitrification system is easily unstable due to changes in ambient temperature

Method used

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  • Method for screening short-cut nitrifying bacteria by using low-intensity ultrasonic waves and application thereof
  • Method for screening short-cut nitrifying bacteria by using low-intensity ultrasonic waves and application thereof
  • Method for screening short-cut nitrifying bacteria by using low-intensity ultrasonic waves and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] 1. Using SBR reactor, such as figure 1 As shown, it is divided into two SBR reactors, the control group and the ultrasonic group, the effective volume of each SBR is 5.0L, and the water exchange rate is 50%.

[0029] 2. Inoculation sludge: Activated sludge from a local sewage treatment plant, control the concentration of MLVSS in the reactor to 2200mg / L;

[0030] 3. Influent water quality: Influent COD, ammonia nitrogen, and total phosphorus are 150, 60, and 1.5 mg / L respectively. By adding NaHCO 3 Solution 600mg / L adjusts the pH in the reactor to be controlled at 8.0;

[0031] 4. Reactor operation mode: intermittent aeration operation mode is adopted, the total aeration time is 150min, divided into five aeration periods, the aeration time is 30min, and the aeration rate is 1.0-1.5L / min. stage ultrasonic irradiation;

[0032] 5. Reactor operating temperature: 18±3°C

[0033] 6. Ultrasonic sound energy density: use a sound energy density of 0.25W / mL to irradiate slud...

Embodiment 2

[0059] 1. Using SBR reactor, such as figure 1 shown. The test is divided into two SBR reactors, the control group and the ultrasonic group, each SBR has an effective volume of 5.0L and a water exchange rate of 50%.

[0060] 2. Inoculation sludge: Activated sludge from a local sewage treatment plant, control the concentration of MLVSS in the reactor to 2000mg / L;

[0061] 3. Influent water quality: Influent COD, ammonia nitrogen, and total phosphorus are 150, 60, and 1.5 mg / L respectively. By adding NaHCO 3 Solution (600mg / L) adjusts the pH in the reactor to be controlled at 7.5;

[0062] 4. Reactor operation mode: intermittent aeration operation mode is adopted, the total aeration time is 150min, divided into five aeration periods, the aeration time is 30min, and the aeration rate is 1.0-1.5L / min. stage ultrasonic irradiation;

[0063] 5. Reactor operating temperature: 25±3°C

[0064] 6. Ultrasonic sound energy density: 0.25W / mL;

[0065] 7. Ultrasonic irradiation time: 1...

Embodiment 3

[0077] 1. Using SBR reactor, such as Figure 6 Shown: The test is divided into two SBR reactors, the control group and the ultrasonic group, the effective volume of each SBR is 5.0L, and the water exchange rate is 60%.

[0078] 2. Inoculation sludge: Activated sludge from a local sewage treatment plant, control the reactor sludge concentration to 2500mg / L;

[0079] 3. Influent water quality: Influent COD, ammonia nitrogen, and total phosphorus are 120, 60, and 6mg / L respectively. By adding NaHCO 3 The solution adjusts the pH in the reactor to be controlled at 8.0;

[0080] 4. Reactor operation mode: the intermittent aeration operation mode is adopted, the total aeration time is 180min, divided into 6 aeration periods, the aeration time is 30min, and the aeration volume in the aerobic stage is 0.2-1.0L / min. Ultrasonic irradiation is carried out during the static stage of the reactor;

[0081] 5. Reactor operating temperature: 30±3°C

[0082] 6. Ultrasonic sound energy densi...

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Abstract

The invention discloses a method for screening short-cut nitrifying bacteria by using low-intensity ultrasonic waves and an application thereof; intermittent low-intensity ultrasonic irradiation is carried out on activated sludge in an SBR, and the ultrasonic single irradiation time, the ultrasonic sound energy density, the ultrasonic mode and the ultrasonic irradiation interval period of the reactor are controlled. The oxygen semi-saturation constant of the short-cut nitrifying bacteria is changed to form a community structure taking the short-cut nitrifying bacteria as dominant bacteria. According to the method, the community structure taking ammonia oxidizing bacteria (AOB) as dominant bacteria are screened out under low-temperature and normal-temperature conditions, screening of short-cut nitrifying bacteria is achieved, and the stability of a short-cut nitrification system is improved; compared with other physical, chemical and biological technologies, the ultrasonic technology cannot cause secondary pollution, and the process is simple and easy to operate. The method can be widely applied to the field of water pollution control.

Description

technical field [0001] The invention belongs to the field of water pollution control, and in particular relates to a method for screening short-range nitrifying bacteria by using low-intensity ultrasonic waves and an application thereof. Background technique [0002] The traditional nitrification and denitrification biological denitrification process was developed earlier and the technology is more mature. The biological denitrification of sewage is realized through the nitrification-denitrification of microorganisms. In recent years, short-range nitrification-denitrification, short-range nitrification-ANAMMOX and other new denitrification processes have become new research hotspots, among which short-range nitrification is the key link for the normal operation of various new denitrification processes. [0003] At present, the methods to achieve short-cut nitrification mainly include controlling dissolved oxygen concentration, pH coupling with free ammonia (FA) and free nitr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C02F3/12C12N1/20C12N13/00
CPCC02F3/1263C12N13/00C12N1/20Y02W10/10
Inventor 朱易春黄书昌任黎晔张立楠连军锋刘祖文
Owner JIANGXI UNIV OF SCI & TECH
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