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Cytobacillus horneckiae strain and use thereof

A cell and bacillus technology, applied in the field of Bacillus horikoshii strains and their uses, can solve problems such as lack of microbial products, and achieve the effects of inhibiting the reproduction of pathogenic bacteria, with good safety and stable effect.

Inactive Publication Date: 2020-12-04
浙江森井生物研究有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

But at present, microbial products that can be widely used and have excellent performance are still relatively lacking.

Method used

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  • Cytobacillus horneckiae strain and use thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] A kind of application of Horikoshi cell bacillus:

[0018] a. Preparation medium: 1% (g / ml) of sucrose, 1% (g / ml) of yeast extract, 0.5% (g / ml) of sodium chloride, supplemented with water, after autoclaving for subsequent use;

[0019] b. Liquid submerged fermentation culture: insert a single colony of the bacterial strain into the above-mentioned culture medium, culture with shaking at 120 r / min, the fermentation temperature is 40°C, the fermentation time is 32 hours, and the OD value of the fermentation broth is measured to be 6;

[0020] c. In 2019, a rice growth promotion test was carried out on the standard planting plot of the China Rice Research Institute in Fuyang District, Hangzhou City, Zhejiang Province. The fermentation liquid of Cytobacter Horikoshi was applied to the soil in the form of base fertilizer, and the fermentation liquid was used per mu of land. 25kg One week after the application, rice seedlings were transplanted (seedling variety: Yongyou 12). ...

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Abstract

The invention relates to a Cytobacillus horneckiae strain and a use thereof. The strain is Cytobacillus horneckiae, a preservation number is CGMCC No.20452, and a 16s rDNA base sequence of the strainis shown as SEQ ID NO: 1. The invention also relates to a method for using the strain in planting. The method comprises the following specific steps: (1) a fermentation culture medium formula; (2) a fermentation method (including fermentation parameters and methods); and (3) a fermentation liquid planting application method. The beneficial effects are that the bacterial strain is colonized in thesoil and occupies the region advantage, so that the propagation of pathogenic bacteria is inhibited, the soil nutrient condition is improved, and the crop growth is promoted.

Description

technical field [0001] The invention relates to a bacterial strain and its application, in particular to a Horikoshi cellobacter strain and its application. Background technique [0002] At present, China is a big producer of chemical fertilizers and pesticides in the world. The model of "fertilizers in charge and pesticides escort" has not only increased China's grain production year after year, but also brought about soil quality decline, serious pests and diseases, decline in the quality of agricultural products, water pollution and even agricultural product safety, etc. A series of major issues. The current extensive agricultural management model is an unsustainable development model. In the future, chemical agricultural production methods must be transformed into ecological agricultural production methods. [0003] Agricultural microbial agents used in planting are often cultivated by bacteria, actinomycetes, fungi and yeasts that are effective and safe for plants. Ag...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/20A01P1/00A01P3/00A01P21/00C12R1/01
CPCA01N63/20C12N1/20C12N1/205C12R2001/01
Inventor 刘侃诚
Owner 浙江森井生物研究有限公司
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