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Method for reinforcing recycled fine aggregate by bacillus pasteurii DSM33

A technology of Bacillus pasteurii and fine aggregate regeneration, which is applied in the intersection of building materials, microbiology and solid waste resource utilization, can solve the problem of limiting the scope of application of the modification process, increasing the potential cost of the modification process, and regenerating fine aggregates. There are few problems such as less aggregate modification, so as to reduce the cost of strengthening operation, improve the modification efficiency and effect, and have good stress resistance.

Active Publication Date: 2020-12-08
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, most of the existing modification processes are aimed at regenerated coarse aggregate, and there is less modification of regenerated fine aggregate.
At the same time, the existing process often needs to cooperate with vibration or add other auxiliary chemical substances to improve the effect, which greatly limits the scope of application of the modification process and increases the potential cost of the modification process
In addition, due to the immaturity of the existing process research, the length of some processes is too long, the modification efficiency is low, and it is not suitable for practical application

Method used

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  • Method for reinforcing recycled fine aggregate by bacillus pasteurii DSM33
  • Method for reinforcing recycled fine aggregate by bacillus pasteurii DSM33
  • Method for reinforcing recycled fine aggregate by bacillus pasteurii DSM33

Examples

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Effect test

Embodiment 1

[0030] This embodiment includes the following steps:

[0031] (1) Weigh 20 g of tryptone, 5 g of sodium chloride, 20 g of urea, and 1000 mL of deionized water to prepare a proliferation medium, and adjust the pH value to 9.5 by adding NaOH solution and HCl solution dropwise. After autoclaving at 121° C. for 20 minutes, Bacillus pasteurianus DSM33 was inoculated, shaken at 30° C. for 24 hours, and the shaking frequency was 130 rpm.

[0032] (2) Pass the regenerated fine aggregate through a 0.075mm sieve and wash it with water. The particle size ranges from 0.075mm to 4.75mm. Weigh 1000g and add it to 1L of the above-mentioned bacterial proliferation culture solution, and culture it statically at 30°C for 24h.

[0033] (3) Weigh 3 g of tryptone, 2.12 g of sodium carbonate, 5 g of ammonium chloride, 20 g of urea, 0.5 mol of calcium acetate, and 1000 mL of deionized water to prepare a precipitation medium. The above-mentioned regenerated fine aggregate was taken out from the bact...

Embodiment 2

[0040] This embodiment includes the following steps:

[0041] (1) Take 20 g / L tryptone, 5 g / L sodium chloride, and 20 g / L urea as standard to weigh and configure the proliferation medium, and adjust the pH value to 9.5 by adding NaOH solution and HCl solution dropwise. After autoclaving at 121° C. for 20 minutes, Bacillus pasteurianus DSM33 was inoculated, shaken at 30° C. for 24 hours, and the shaking frequency was 130 rpm.

[0042] (2) Prepare construction waste into recycled fine aggregate and wash it with water. The sieved particle size ranges from 0.6mm to 4.75mm. The ratio of aggregate to proliferation medium is 100g:100mL, and the aggregate is put into bacterial proliferation culture medium cultured at 30°C for 24 hours.

[0043] (3) Take tryptone 3g / L, sodium carbonate 2.12g / L, ammonium chloride 5g / L, urea 20g / L, calcium acetate 0.5mol / L as the standard and prepare the precipitation medium. The above-mentioned regenerated fine aggregate is taken out from the bacterial ...

Embodiment 3

[0051] This embodiment includes the following steps:

[0052] (1) Take 20 g / L tryptone, 5 g / L sodium chloride, and 20 g / L urea as standard to weigh and configure the proliferation medium, and adjust the pH value to 9.5 by adding NaOH solution and HCl solution dropwise. After autoclaving at 121° C. for 20 minutes, Bacillus pasteurianus DSM33 was inoculated, shaken at 30° C. for 24 hours, and the shaking frequency was 130 rpm.

[0053] (2) The construction waste is prepared into recycled fine aggregate and washed with water, and the sieved particle size ranges from 0.075 mm to 4.75 mm, and is equally divided into two parts for later use. And take tryptone 3g / L, sodium carbonate 2.12g / L, ammonium chloride 5g / L, urea 20g / L, calcium acetate 0.5mol / L as the standard, weigh and configure the precipitation medium, set aside.

[0054] (3) The first aggregate is recorded as one-time modified aggregate, and the ratio of aggregate and proliferation medium is 100g:100mL. Put the aggregate...

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Abstract

The invention provides a method for reinforcing recycled fine aggregate by using bacillus pasteurii DSM33. The method comprises the following steps: preparing a proliferation culture medium, dropwiseadding a NaOH solution and an HCl solution to regulate the pH value to 9.0-9.5, sterilizing, cooling to room temperature, inoculating bacillus pasteurii, and carrying out shake culture at 28-32 DEG Cat the rotating speed of 130 rpm to obtain a proliferated bacterial culture solution; adding dried recycled fine aggregate into the proliferated bacterial culture solution, and standing at the temperature of 28-32 DEG C for culture; taking out the soaked recycled fine aggregate, putting into a precipitation culture medium, and standing at the temperature of 28-32 DEG C for culture; completing theoperation, and drying the recycled fine aggregate to obtain primarily reinforced recycled fine aggregate; and the aggregate subjected to primary reinforcement can be repeatedly reinforced. The weightof the recycled fine aggregate prepared by the method is increased, and the water absorption rate is remarkably reduced. Recycled mortar prepared from the reinforced recycled fine aggregate has significantly improved compression strength and flexural strength, and significantly improved shrinkage performance and carbonization resistance.

Description

technical field [0001] The invention relates to the interdisciplinary technical field of building materials, microbiology and solid waste resource utilization, in particular to a method for strengthening regenerated fine aggregate with Bacillus pasteurianum DSM33. Background technique [0002] my country is currently in the process of vigorous development of the construction industry and the construction of a large number of urban infrastructure. As the most important building material, the average annual output of commercial concrete has exceeded 2.5×10 9 m 3 , which means the rapid consumption of natural sand and gravel resources, and about 32 billion to 50 billion tons of sand and gravel resources in the world are used for industrial production every year. At the same time, it cannot be ignored that the annual construction waste generated in my country has exceeded 3 billion tons, and the utilization rate of resources is less than 10%. The shortage of resources and the ...

Claims

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Application Information

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IPC IPC(8): C04B20/02
CPCC04B20/023C04B18/16
Inventor 赵羽习冯璋曜曾维来
Owner ZHEJIANG UNIV
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