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A deep-sea fish skin collagen peptide and its extraction and preparation method

A technology of fish skin collagen and deep-sea fish, applied in the biological field, can solve problems such as unsuitable for industrial scale, not environmentally friendly extraction methods, and low purity of protein peptides, achieve good commercial application value, improve preparation efficiency and quality, and have high purity Effect

Active Publication Date: 2021-02-23
JIANGXI UNIVERSITY OF TRADITIONAL CHINESE MEDICINE +3
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] At present, there are few domestic methods for extracting low-molecular-weight protein peptides from deep-sea fish skin. The main difficulties are low extraction efficiency, low purity of protein peptides, extraction methods that are not environmentally friendly and not suitable for industrial scale, etc.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] This embodiment proposes a method for extracting and preparing deep-sea fish skin collagen peptides, comprising the following steps:

[0027] S1. First remove the head of the fresh deep-sea cod, take its skin and clean it, weigh 2.0kg of the cod skin, soak it in 4mol / L baking soda for 40min for deodorization pretreatment; then put it into a concentration of 0.3 After soaking in mol / L NaOH solution for 30min, rinse with water.

[0028] S2. Put the deep-sea fish skin treated in the step S1 into a beater, add pure water for beating for 5 minutes, the volume ratio of pure water to deep-sea fish skin is 3:1, and obtain a deep-sea fish skin homogenate.

[0029] S3. Adding pepsin to the deep-sea fish skin homogenate, the mass ratio of pepsin to the deep-sea fish skin homogenate is 1:100, the reaction conditions are: pH 1.5, temperature 35°C, enzymolysis time 5 hours; Then add papain, the mass ratio of papain to deep-sea fish skin homogenate is 1:100, the reaction conditions a...

Embodiment 2

[0032] This embodiment proposes a method for extracting and preparing deep-sea fish skin collagen peptides, comprising the following steps:

[0033] S1. First remove the head of the fresh deep-sea cod and take its skin to clean, weigh 2.0kg of the cod skin, put it in a concentration of 4.2mol / L baking soda and soak it for 35min for deodorization pretreatment; then put it into a concentration of After soaking in 0.4mol / L NaOH solution for 32min, rinse with water.

[0034] S2. Put the deep-sea fish skin treated in the step S1 into a beater, add pure water for beating for 5 minutes, the volume ratio of pure water to deep-sea fish skin is 3.5:1, and obtain a deep-sea fish skin homogenate.

[0035]S3. Add pepsin to the deep-sea fish skin homogenate, the mass ratio of pepsin to deep-sea fish skin homogenate is 1:150, the reaction conditions are: pH 2, temperature 36°C, enzymatic hydrolysis time 6 h ; then add papain, the mass ratio of papain to deep-sea fish skin homogenate is 1:15...

Embodiment 3

[0038] This embodiment proposes a method for extracting and preparing deep-sea fish skin collagen peptides, comprising the following steps:

[0039] S1. First remove the head of fresh deep-sea cod and take its skin to clean, weigh 2.0kg of cod skin, put it in 4.5mol / L baking soda for 30min for deodorization pretreatment; then put it into After soaking in 0.5mol / L NaOH solution for 35min, rinse with water.

[0040] S2. Put the deep-sea fish skin treated in the step S1 into a beater, add pure water for beating for 5 minutes, the volume ratio of pure water to deep-sea fish skin is 4:1, and obtain a deep-sea fish skin homogenate.

[0041] S3. Pepsin is added to the deep-sea fish skin homogenate, and the mass ratio of pepsin to the deep-sea fish skin homogenate is 1:200. The reaction conditions are: pH 2.5, temperature 38°C, enzymolysis time 7 h; then add papain, the mass ratio of papain to deep-sea fish skin homogenate is 1:200, the reaction conditions are: pH 6, The temperature...

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Abstract

The invention discloses a method for extracting and preparing collagen peptide from deep-sea fish skin, and relates to the field of biotechnology, including: S1, performing deodorization pretreatment on deep-sea fish skin; S2, beating the fish skin after step S1 processing to obtain a deep-sea fish skin homogenate; S3, adding pepsin to the deep-sea fish skin homogenate for enzymolysis, the enzymolysis pH is 1.5-3; adding papain for enzymolysis, the enzymolysis pH is 5.5-7; Adding trypsin for enzymolysis, the pH of the enzymolysis is 7.5-8.5 to obtain an enzymolysis mixture; S4, inactivating, purifying and drying the enzymolysis mixture to obtain deep-sea fish skin collagen peptides. The beneficial effect of the present invention is that the fish skin is placed in acidic pepsin, neutral papain and alkaline trypsin in sequence, and the fish skin is fully enzymolyzed by the three enzymes, so that the The protein is hydrolyzed to obtain the peptide, which improves the extraction rate of the final protein peptide.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a deep-sea fish skin collagen peptide and an extraction and preparation method thereof. Background technique [0002] Collagen is a biological polymer, the main component of animal connective tissue, and also the most abundant and widely distributed functional protein in mammals, accounting for 25-30% of the total protein, and even as high as 80% in some organisms. Collagen is an important component of the skin, and about 76% of the dry weight of the average person's skin is collagen. Collagen plays a supporting role in the skin, which can lock moisture inside and prevent water loss. This shows the importance of collagen to the skin, so supplementing collagen can avoid many skin problems. [0003] Modern biological metabolism research shows that after protein digestion, it is mainly directly absorbed in the form of polypeptides. For example, fish skin collagen polypeptides not only ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K14/78C12P21/06C07K1/34C07K1/14
CPCC07K14/78C12P21/06
Inventor 欧阳辉冯育林何明珍黄光春龙威
Owner JIANGXI UNIVERSITY OF TRADITIONAL CHINESE MEDICINE