Application of Map2k3 or Map2k6 in improvement of efficiency of inducing adult cells to generate pluripotent stem cells

Abstract

The invention provides application of Map2k3 or Map2k6 in improvement of the efficiency of inducing adult cells to generate pluripotent stem cells, a method for improving the efficiency of inducing the adult cells to generate the pluripotent stem cells and a kit. The method comprises the following step: introducing Map2k3 or Map2k6 and transcription factors into mammalian adult cells for induced culture to obtain the pluripotent stem cells, wherein the stem cell induction factors are Oct4, Klf4 and Sox2, or Oct4, Klf4, Sox2 and c-Myc. The method further comprises the step of adding vitamin C into an induction medium, and the efficiency of inducing the pluripotent stem cells is further improved compared with the method without adding the vitamin C. According to the application, the efficiency of inducing the pluripotent stem cells is improved by adopting the kinase gene Map2k3 or Map2k6 and the transcription factors, so that the high-quality induced pluripotent stem cells with reproductive system transmission capacity are obtained.

Description

Technical field [0001] The invention relates to the field of biology. Particularly, the invention relates to the application of Map2k3 or Map2k6 in improving the efficiency of inducing adult cells to generate pluripotent stem cells. technical background [0002] Human embryonic stem cells (hESCs) are a kind of totipotent stem cells, which have the potential of infinite proliferation, self-renewal and multi-directional differentiation in vitro. Theoretically, whether in vitro or in vivo, hESCs can be induced to differentiate into almost all cell types of human body. Further research and application of hESCs will open up a new way for transplantation therapy and treatment of macular degeneration, Parkinson's disease, amyotrophic lateral sclerosis and other difficult diseases. However, because hESCs are difficult to obtain and involve sensitive ethical issues, the clinical research of hESCs was once in an awkward position and could not be carried out on a large scale. [0003] In 20...

AI Technical Summary

Problems solved by technology

[0004] In the process of inducing iPS, exogenous genes are often introduced into cells by means of retroviruses and lentiviruses. In this way, high gene transduction efficiency can be obtained, but the integration of viral sequences into the genome of cells will lead to gene insertion mutations. , even carcinogenic, so this potentially dangerous gene transfer method is obviously not conducive to the application of iPS technology in the field of regenerative medicine. Therefore, different research groups have used non-integrating vectors to induce iPS and achieved success. These vectors include Adenoviral vectors, general expression vectors, transposons, episomal vectors, minicircle DNA (minicircleDNA) vectors

[0005] At present, the combinations of transcription factors capable of reprogramming include Oct4, Klf4, Sox2, c-Myc; Oct4, Nanog, Lin28, Sox2; Sox2, Klf4, and Lrh1; Related genes, the combination of transcription factors required by the existing reprogramming method needs to import more transcription factors, and the induction efficiency is low. It is of great significance to study the mechanism of reprogramming and the improvement of reprogramming technology by screening highly efficient non-integrating vectors and compounds that replace transcription factors

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