Kit for dual detection of viruses by nucleic acid and antibodies and preparation method thereof

Abstract

The invention relates to a kit for dual detection of viruses by nucleic acid and antibodies and preparation method thereof. According to the invention, an RPA primer and a probe which specifically aimat H1N1 detection are obtained by analyzing an H1N1 sequence, and the RPA primer and the probe are prepared into a corresponding detection test strip. At the same time, monoclonal antibodies which have good effects are screened and obtained aiming at an H1N1 conserved region. An H1N1 influenza virus fluorescence quantum dot rapid detection test paper is prepared from a monoclonal antibody labeledquantum dot and other antibody labeled nitrocellulose membranes. The two detection methods are combined for use, so that the detection accuracy can be further improved, the cost is low, and the kit and method is suitable for large-scale popularization.

Description

technical field

[0001] The invention relates to the detection field, in particular to a nucleic acid-antibody dual-detection virus kit and a preparation method thereof. Background technique

[0002] Influenza virus (abbreviated as influenza virus) is a RNA virus that causes influenza in humans and animals. It belongs to the Orthomyxoviridae family and is divided into three types: A, B, and C. It is spherical or filamentous, with a diameter of 80-120nm. Type 3 viruses have similar biochemical and biological characteristics. The various subtypes of influenza A virus are divided according to the antigenicity of virion hemagglutinin and neuraminidase. Influenza virus causes acute upper respiratory tract infection, which spreads rapidly mainly through the patient's droplets, contact between patients or contact with contaminated objects. Influenza A virus spreads more rapidly, and the incubation period after infection is very short. , can also infect mammals and birds; the antig...

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