Nematicidal composition for meloidogyne incognita and application thereof
A technology for killing nematodes and nematodes of southern root-knot nematode is applied in the field of pesticide application, which can solve the problems that the actual efficacy of the pesticide composition cannot be confirmed, the actual efficacy cannot be verified, and the like, and achieves a technology that is conducive to drug resistance control and delaying drug resistance. The effect of generating and developing, reducing pollution
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Embodiment 1
[0038] This example is an indoor toxicological determination of thiazophosphine and imidacloprid single agent and various ratio mixtures.
[0039] (1) Material of root-knot nematode incognita
[0040] Meloidogyne incognita populations were collected from Nanjing, Jiangsu, and kept in the greenhouse of the Jiangsu Academy of Agricultural Sciences with the tomato Moneymaker variety as the host. Meloidogyne incognita egg masses were isolated, and the oocysts were hatched in an indoor incubator to obtain a large number of indoor toxicological bioassay sources of M. incognita. The bioassay object was the 2nd instar larva of M. incognita.
[0041] (2) Isolation of Meloidogyne incognita
[0042] Select tomato plants with obvious root knots, wash off the root sediment, chop the roots with scissors, put them in a 500mL sealed bottle, add 10% bleach and shake vigorously for 5min. The nematodes and nematode eggs in the root tissue were collected by filtering through 80-mesh, 300-mesh a...
Embodiment 2
[0060] This embodiment is an indoor pot test of the compound agent of thiazophos and imidacloprid.
[0061] The optimum mass ratio of thiazophosphine and imidacloprid screened out according to the results of indoor toxicity determination in Example 1 is 1:3, and the root-knot nematode on tomato is used as the control object, and the mixed nematocide of thiazophosphine and imidacloprid is used for indoor potting test. The specific experimental method is as follows:
[0062] (1) Material of root-knot nematode incognita
[0063] The root-knot nematode colony was raised in the greenhouse of the Jiangsu Academy of Agricultural Sciences, with the tomato Moneymaker variety as the host, the greenhouse temperature was 28±2°C, the relative humidity was above 70%, 14 hours of light: 10 hours of darkness, and tomatoes with obvious root knots were selected. Plants, isolate the egg masses of M. incognita, hatch the oocysts in an indoor thermostat, and obtain a large number of test insect ...
Embodiment 3
[0089] This example is a field efficacy test of the compound of thiazophos and imidacloprid.
[0090] The optimal mass ratio of thiazophos and imidacloprid 1:3 and the results of the pot plant test of embodiment 2 screened out according to the results of indoor toxicity determination in Example 1, further take the root-knot nematode on tomato as the control object, to thiazophos and imidacloprid Mixed nematicides were used for field efficacy test. The specific experimental method is as follows:
[0091] (1) Preparations
[0092] 20% thiazophosin water emulsion, commercially available; 70% imidacloprid water-dispersible granules, commercially available; thiazophosin-imidacloprid compound (wherein the mass ratio of thiazophosinate to imidacloprid is 1:3), prepared by tank mixing.
[0093] (2) Test objects and conditions
[0094] The control object is root-knot nematode incognita on tomato, and the fields where root-knot nematode incognita occurs heavily all the year round are...
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