Mitoxantrone and berberine composition and application thereof in preparation of anti-tumor drugs
A technology of mitoxantrone and a composition is applied in the application field of a mitoxantrone and berberine composition in reducing the cardiotoxicity of mitoxantrone, and achieves easy industrialization, good stability, and reduced cardiotoxicity. Effect
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Embodiment 1
[0041] Example 1: In vitro cytotoxicity test
[0042] MTT (thiazolium blue) method was used to screen the synergistic ratio of mitoxantrone and berberine to mouse breast cancer (4T1) cells.
[0043] Dilute 4T1 cells with culture medium to a density of 1×10 4 cells / mL, and inoculated in a 96-well plate, 100 μL per well. After overnight incubation in the incubator, discard the old culture medium, add different concentrations of mitoxantrone hydrochloride solution, berberine hydrochloride solution and different ratios of mitoxantrone hydrochloride-hydrochloride diluted with fresh culture medium to each well The berberine mixed solution was placed in an incubator and incubated for 48 hours. Afterwards, the cell viability at each concentration was calculated by the MTT method. The combination index (CI) of mitoxantrone and berberine was calculated by the Chou-Talalay method, and the characteristics of their combined effects were investigated. The formula for calculating CI is: ...
Embodiment 2
[0047]MTT method was used to screen the synergistic ratio of mitoxantrone and berberine on mouse leukemia (L1210) cells.
[0048] This example is the same as Example 1, except that the density of L1210 cells after dilution is 3×10 4 cells / mL, see the experimental results figure 2 . The experimental results showed that the synergistic effect of mitoxantrone and berberine on 4T1 cells was not exactly the same as that of L1210 cells, and the mass ratio of mitoxantrone: berberine in the range of 1:2-1:10 showed a relatively strong effect. Good synergy.
Embodiment 3
[0049] Embodiment 3: Preparation of double drug-loaded liposomes
[0050] The preparation method of the double drug-loaded liposome of the present embodiment comprises the following steps:
[0051] (1) Weigh hydrogenated soybean lecithin, cholesterol, methoxy-polyethylene glycol 2000-distearoylphosphatidylethanolamine (mass ratio 3:1:1), add chloroform to dissolve, and evaporate under reduced pressure to remove the organic solvent to form a dry lipid film;
[0052] (2) After adding 300mM ammonium sulfate solution and hydrating at 65°C for 30min, carry out granulation through polycarbonate membrane to form nano-sized small unicellular liposomes with ammonium sulfate solution inside and outside the liposome membrane;
[0053] (3) above-mentioned small unicellular liposomes are passed through the agarose CL-4B gel column equilibrated with the PBS solution (pH 7.5) of 10mM histidine in advance to form ammonium sulfate gradient blank liposomes;
[0054] (4) Drug loading process: ...
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