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Novel gradient hydrogel material, and preparation method and application thereof

A hydrogel and gradient technology, applied in the direction of biochemical equipment and methods, bone/connective tissue cells, animal cells, etc., can solve the limitation of simulation, there is no research on the preparation of continuous mechanical gradient hydrogel materials, and there is no gradient structure and other issues to achieve the effect of good biocompatibility

Active Publication Date: 2020-12-25
南京大学深圳研究院
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Problems solved by technology

However, the current conventional biogel materials do not have a gradient structure, which is very different from the continuous gradient structure of natural biological tissues.
And so far, there is still no research to prepare a continuous mechanical gradient hydrogel material for the biomedical field, which limits the simulation of biological tissue in terms of gradient structure of the gel material, so the development of a new type of bio-friendly mechanical gradient hydrogel Gel matrix materials are also a problem that needs to be solved in the field of cell culture matrix materials and platforms

Method used

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  • Novel gradient hydrogel material, and preparation method and application thereof
  • Novel gradient hydrogel material, and preparation method and application thereof
  • Novel gradient hydrogel material, and preparation method and application thereof

Examples

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preparation example Construction

[0034] A preparation method of a novel gradient hydrogel material, the steps are as follows:

[0035] S1. Preparation of collagen solution: acrylamide (101.25mg), acrylic acid-PH 6Peptide (27mg), LAP (0.225mg) were dissolved in Tris buffer (0.45mL, 1M Tris and 0.3M KCL, pH=7.6); the mixed solution was divided into three parts, and then 3mg, 6mg and 9mg double bond modified multi-arm polyethylene glycol, to obtain three kinds of double bond modified multi-arm polyethylene glycol molecules with different collagen solutions;

[0036] S2. Preparation of new gradient hydrogel materials: Add the three kinds of collagen solutions in step S1 into the glass mold at a uniform speed in order of the molecular concentration of multi-armed polyethylene glycol from high to low, and place the mold under ultraviolet light (365nm, 8W) for 2 hours; soak the polymerized gradient hydrogel in Tris buffer for 24 hours, and then use a mechanical tester to drive the hydrogel at a concentration of 0.8...

Embodiment 1

[0042] Example 1 The present invention is tested in terms of mechanical strength and energy dissipation.

[0043] In terms of mechanical strength and energy dissipation, we used a biomechanical testing machine to carry out compression tests on the gradient hydrogel in this design, such as image 3 shown. image 3 A shows that after gradient immersion in zinc chloride solution, the Young's modulus of each position of the gradient hydrogel remains at 140kPa, and the whole has uniform mechanical strength. Further, in the compression cycle experiment ( image 3 B-E), the fracture deformation of the hydrogel at 0cm is 70% of the original length, and the fracture stress is less than 200kPa; while the fracture deformation of the hydrogel at 8cm is 90% of the original length, and the fracture stress is greater than 1200kPa. Compared with the energy dissipation and toughness of the hydrogel at 8 cm, the energy dissipation of the hydrogel at 0 cm increased by 2 times, and the toughnes...

Embodiment 2

[0044] Example 2 The invention was tested in terms of stress relaxation.

[0045] In terms of stress relaxation, we used a biomechanical testing machine to conduct a compressive stress relaxation test on the gradient hydrogel in this design, as shown in Figure 4 shown. The stress acting on the hydrogel at 0 cm (light blue line) decreased by 11 kPa in 30 min and the stress on the hydrogel at 8 cm (dark blue line) decreased by 35 kPa in 30 min. It can be seen that under the same strain load, the hydrogel at 8 cm exhibits a greater degree of stress relaxation, and its stress relaxation degree is three times that of the hydrogel at 0 cm. In addition, the stress relaxation speed of the hydrogel at 8 cm is faster, and the stress relaxation speed of the hydrogel at 0 cm is slightly slower ( Figure 4 C and D). This indicates that both the extent and speed of stress relaxation in hydrogels increase with the increase in the density of coordinative crosslinks and the decrease in the...

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Abstract

The invention discloses a novel gradient hydrogel material, and a preparation method and application thereof. The novel gradient hydrogel material comprises multi-arm super-hydrophilic macromolecules,polypeptide ligand molecules, linear macromolecules and metal ions. The novel gradient hydrogel material based on a hydrophilic polymer skeleton can be widely applied to the aspects of cell culture,signal path research and the like, and is suitable for researching the influence of changes of substrate stress relaxation properties and continuous stress relaxation gradients on cell life behaviorsand exploring a mechanism of a force signal path of cells.

Description

technical field [0001] The invention relates to a novel gradient hydrogel material and its preparation method and application. Background technique [0002] The extracellular matrix supports cells and connects them together to form tissues and organs. At the same time, the extracellular matrix also transmits signals to cells and regulates cell growth, polarity, shape, migration and metabolic activities. The study of biological signals that regulate cell life activities is of great significance to the development of biomedicine. In recent years, researchers have discovered that mechanical signals can regulate the life activities of cells, which has aroused great interest. However, the natural extracellular matrix usually has fixed components, and it is difficult for people to easily control its mechanical properties. Hydrogel is composed of a network formed by the cross-linking of hydrophilic macromolecules, contains a large amount of water inside, has a microstructure sim...

Claims

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Application Information

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IPC IPC(8): C08J3/075C08F290/06C08F220/56C08F220/60C12N5/077C12N5/00C08L51/08
CPCC08J3/075C08F290/062C12N5/0062C12N5/0656C08J2351/08C12N2533/30C12N2513/00C08F220/56C08F220/603
Inventor 薛斌于文婷曹毅秦猛王炜
Owner 南京大学深圳研究院
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