Purification method of oxytocin

Abstract

The invention relates to the technical field of drug synthesis, and discloses a purification method of oxytocin. The purification method comprises the following steps: S1, selecting an oxytocin crudeproduct solution which is qualified through detection; and S2, carrying out reversed-phase purification on the oxytocin crude product solution by a reversed-phase high performance liquid chromatography, wherein the reversed-phase purification conditions are as follows: a chromatographic packing of the reversed-phase high performance liquid chromatography is C18; mobile phase composition is as follows: a mobile phase A is ammonium sulfate with the concentration of 30-80 mmol / L, the pH value of sulfuric acid is 2.0-5.0, and a mobile phase B is acetonitrile; and elution conditions are as follows:a gradient method is used, and fractions at 14-20% of B and the wavelength of 220 nm are collected in sections. A qualified sample with the purity greater than or equal to 99.5% and the single impurity less than or equal to 0.1% can be obtained through reversed-phase one-step purification; Reversed-phase salt conversion is only carried out, the sample loading amount is high (DAC-150 can load 30 gof refined peptide), high-gradient elution is carried out to obtain a qualified liquid with small volume and high concentration, later rotary evaporation and concentration are facilitated, residual ions after salt conversion are low, the total purification yield of reversed-phase purification and reversed-phase salt conversion is high (81.83%-89.71%), and the cost is reduced.

Description

technical field [0001] The invention relates to the technical field of medicine synthesis, in particular to a method for purifying oxytocin. Background technique [0002] Drugs are substances used to prevent, treat and diagnose diseases. In theory, drugs refer to all chemical substances that can affect the physiological functions of the body's organs and cell metabolism activities. Oxytocin is one of them, which mainly stimulates the breast to secrete milk, promotes the contraction of uterine smooth muscle during childbirth, and promotes the role of maternal love. In addition, it can also reduce stress hormones such as adrenal ketone in the human body. In order to lower blood pressure, in the process of preparing oxytocin, the qualified liquid obtained has a large volume and low concentration, which is not conducive to the later rotary evaporation and concentration, and the residual ions after salt conversion are high, so the obtained liquid needs to be adjusted Carrying ou...

AI Technical Summary

Problems solved by technology

[0004] Aiming at the deficiencies of the prior art, the present invention provides a method for purifying oxytocin, which solves the problem that in the process of preparing oxytocin, the obtained qualified liquid has a large volume and low concentration, which is not conducive to subsequent rotary evaporation and concentration. After salt conversion, the residual ions are high, and the obtained liquid needs to be further processed, which greatly increases the problem of manufacturing cost