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Aniline high-efficiency degradation microbial agent, preparation method thereof and application of aniline high-efficiency degradation microbial agent in chemical wastewater

A technology for efficiently degrading bacteria and chemical wastewater, applied in biochemical equipment and methods, chemical instruments and methods, and methods based on microorganisms, etc. The effect of high capacity and good aniline degradation performance

Inactive Publication Date: 2021-01-05
JIANGSU LASON CHEM ENVIRONMENTAL PROTECTION
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

A number of research results show that the biological method has also made outstanding progress in the degradation of aniline. A variety of microorganisms such as Bacillus and Pseudomonas have been isolated, and under aerobic and anaerobic conditions. Concentrated aniline shows better aniline degradation efficiency, but at high aniline concentration, it is difficult to achieve good results in a short time

Method used

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  • Aniline high-efficiency degradation microbial agent, preparation method thereof and application of aniline high-efficiency degradation microbial agent in chemical wastewater

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preparation example Construction

[0027] The main components of the high-efficiency aniline-degrading bacterial agent of the present invention are Alcaligenes faecalis, Bacteroides giesii and Bacillus lysinus. The preparation method of the highly efficient aniline degradation bacterial agent comprises the following sequence and steps:

[0028] Step 1. First inoculate the obtained Alcaligenes faecalis, Bacteroides gisneri and Bacillus lysinus respectively in the enrichment medium, and shake and culture to the logarithmic phase; the formula of the enrichment medium is: Beef extract 3.0 g / L; peptone 10.0 g / L; NaCl 5.0 g / L.

[0029] Step 2, insert the seed liquid of each bacterial strain described in step 1 into the seed liquid culture medium according to the inoculation amount of 10% and carry out expanded cultivation; The formula of the seed liquid culture medium is: aniline 100mg / L; NaCl 1 g / L; K 2 HPO 4 1 g / L; KH 2 PO 4 1 g / L; trace element solution 10 mL, pH=7.0.

[0030] Step 3, inoculate the culture ...

Embodiment 1

[0034] Add the produced high-efficiency aniline-degrading bacteria agent to the inorganic salt medium containing 200mg / L, 400mg / L, 600mg / L, 800mg / L, and 1000mg / L according to the dosage of 1‰, and shake it at 37°C After culturing, samples were taken at different time periods to determine the remaining aniline content. The result is as figure 1 As shown, at the concentration of 200mg / L aniline, it can be reduced to below 25mg / L in 24 hours, and can be completely degraded in 48 hours; at the concentration of 400~1000mg / L aniline, it can be degraded to below 50mg / L in 72 hours.

Embodiment 2

[0036] The high-efficiency aniline-degrading bacterial agent produced was added to a certain pesticide wastewater containing 1000mg / L according to the dosage of 1‰, cultured on a shaking table at 37°C, samples were taken at different time periods, and the remaining aniline content was determined. The results showed that the concentration of aniline could be degraded to less than 50mg / L in 72 hours, and the removal rate was over 95%.

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Abstract

The invention discloses an aniline high-efficiency degradation microbial agent, a preparation method thereof and application of the aniline high-efficiency degradation microbial agent in chemical wastewater. The aniline high-efficiency degradation microbial agent mainly comprises alcaligenes faecalis, microbacterium bacteroides distasonis and lysine bacillus. The aniline degrading microbial agentcan grow by taking aniline as a unique carbon source and a unique nitrogen source, and can degrade 1000mg / L of aniline to 50mg / L or below, and the removal rate reaches 95% or above; the microbial agent prepared by the invention is added into wastewater according to a certain amount, contains a plurality of microorganisms with excellent degradation capability on aniline, and has a good degradationeffect on aniline polluted water, so that the problem that aniline is difficult to biologically solve is solved.

Description

technical field [0001] The invention belongs to the field of wastewater treatment by biological methods, and in particular relates to the preparation and application of a composite bacterial agent for treating aniline wastewater. Background technique [0002] Aniline is a kind of light yellow or colorless oily liquid with a strong smell, mainly used in the manufacture of drugs, resins, dyes and rubber vulcanization accelerators, etc. Up to thousands of milligrams per liter. However, when the concentration of aniline in wastewater is higher than 100mg / L, it will pose a serious threat to aquatic organisms and human health, so aniline or its derivatives have been included in the "priority pollutant blacklist" by the EPA and China's environmental protection department. The top priority of water pollution control in the environmental protection industry. [0003] Aniline is a kind of refractory organic matter, and the methods to deal with it are generally divided into chemical ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C02F3/34C12R1/05C12R1/01C12R1/07C02F101/38
CPCC12N1/20C02F3/34C02F2101/38
Inventor 蔡凯邵如英王镔赵振华高峰王帅杜利民
Owner JIANGSU LASON CHEM ENVIRONMENTAL PROTECTION
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