Preparation method of levodopa nanoparticles and biosensing application of levodopa nanoparticles

A dopa nano-sensing technology, which is applied in the field of preparation of levodopa nanoparticles, can solve the problems of uncertain emission area, low quantum yield, limited biological sensing and image processing efficiency, etc., and achieves a simple and environmentally friendly synthesis process. , good biocompatibility, the effect of reducing background fluorescence
CN112179879AActive Publication Date: 2021-01-05NANJING UNIV OF TECH
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Patent Information

Authority / Receiving Office
CN Β· China
Patent Type
Applications(China)
Current Assignee / Owner
NANJING UNIV OF TECH
Publication Date
2021-01-05

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Abstract

The invention relates to a preparation method of levodopa nanoparticles and biosensing application of the levodopa nanoparticles, and belongs to the technical field of nano biosensing. Levodopa (L-DA)is used as a unique precursor to synthesize levodopa nanoparticles (LNDS) at room temperature, and the formed LNDS can quickly and effectively quench a dye-labeled oligonucleotide fluorescent probe (FAM-DNA) through electrostatic interaction. Complementary deoxyribonucleic acid (cDNA) or adenosine triphosphate (ATP) is competitively combined with a fluorescent probe, so that fluorescence is recovered, and an effective sensing platform for detecting biomolecules is constructed. In addition, the sensing platform can also perform high-sensitivity and selective detection on adenosine triphosphatein a complex system (human serum). The synthetic process of the levodopa nanoparticles is simple and environmentally friendly, the toxic and side effects on cells and organisms are low, the levodopananoparticles have a very strong quenching effect on dye-labeled oligonucleotides, the background fluorescence is greatly reduced, and the signal-to-noise ratio is increased.
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Description

technical field

[0001] The invention relates to a preparation method of levodopa nanoparticles and its application in biosensing, belonging to the technical field of nano-biosensing. Background technique

[0002] Carbon-based nanomaterials, including carbon nanotubes and graphene, hold great promise in electronic, chemical, and biological applications due to their excellent thermal, electronic, and mechanical properties. These nanostructures have been shown to act as "nanoscaffolds" for oligonucleotides and "nanoquenchers" for dyes. Therefore, sensors based on carbon-based nanomaterials are often used for fluorescence detection of nucleic acids, proteins, small molecules, and metal ions. Although these nanomaterials show clear advantages in biosensor design, the hydrophobicity and large specific surface area of ​​graphite create strong Ο€-Ο€ stacking interactions with the adsorption of oligonucleotides, resulting in a high energy barrier that eliminates the Possibility to ob...

Claims

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