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Preparation method of levodopa nanoparticles and biosensing application of levodopa nanoparticles

A dopa nano-sensing technology, which is applied in the field of preparation of levodopa nanoparticles, can solve the problems of uncertain emission area, low quantum yield, limited biological sensing and image processing efficiency, etc., and achieves a simple and environmentally friendly synthesis process. , good biocompatibility, the effect of reducing background fluorescence

Active Publication Date: 2021-01-05
NANJING UNIV OF TECH
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  • Description
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  • Application Information

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Problems solved by technology

However, the carbon nanoparticles prepared by common routes have wide size distribution, uncertain emission regions, and low quantum yields, which limit the efficiency of biosensing and image processing.

Method used

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  • Preparation method of levodopa nanoparticles and biosensing application of levodopa nanoparticles
  • Preparation method of levodopa nanoparticles and biosensing application of levodopa nanoparticles
  • Preparation method of levodopa nanoparticles and biosensing application of levodopa nanoparticles

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Effect test

Embodiment 1

[0047] Embodiment 1: At room temperature, get levodopa of 0.02957g and 15mL deionized water and be configured into a mixed solution and be placed in a 20mL glass bottle, so that the concentration of levodopa in the mixed solution is 10mM and placed in a dark room for 1 month, and the color of the solution changes from none A color change to tan indicates the formation of levodopa nanoparticles. The absorption spectrum curve was drawn by UV-Vis spectrophotometer measurement, such as figure 1 .

Embodiment 2

[0048] Embodiment 2: at room temperature, get levodopa of 0.02957g and 15mL deionized water and be configured into a mixed solution and be placed in a 20mL glass bottle, make the concentration of levodopa in the mixed solution be 10mM and place it in a dark room for 3 months, and the color of the solution changes from none to A color change to tan indicates the formation of levodopa nanoparticles. The absorption spectrum curve is drawn by UV-Vis spectrophotometer measurement, such as figure 2 .

Embodiment 3

[0049] Embodiment 3: at room temperature, get levodopa of 0.02957g and 15mL deionized water and be configured into a mixed solution and be placed in a 20mL glass bottle, make the concentration of levodopa in the mixed solution be 0.01M and place it in a dark room for 6 months, and the color of the solution is as follows: A colorless change to tan indicates the formation of levodopa nanoparticles. The absorption spectrum curve is drawn by UV-Vis spectrophotometer measurement, such as image 3 .

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Abstract

The invention relates to a preparation method of levodopa nanoparticles and biosensing application of the levodopa nanoparticles, and belongs to the technical field of nano biosensing. Levodopa (L-DA)is used as a unique precursor to synthesize levodopa nanoparticles (LNDS) at room temperature, and the formed LNDS can quickly and effectively quench a dye-labeled oligonucleotide fluorescent probe (FAM-DNA) through electrostatic interaction. Complementary deoxyribonucleic acid (cDNA) or adenosine triphosphate (ATP) is competitively combined with a fluorescent probe, so that fluorescence is recovered, and an effective sensing platform for detecting biomolecules is constructed. In addition, the sensing platform can also perform high-sensitivity and selective detection on adenosine triphosphatein a complex system (human serum). The synthetic process of the levodopa nanoparticles is simple and environmentally friendly, the toxic and side effects on cells and organisms are low, the levodopananoparticles have a very strong quenching effect on dye-labeled oligonucleotides, the background fluorescence is greatly reduced, and the signal-to-noise ratio is increased.

Description

technical field [0001] The invention relates to a preparation method of levodopa nanoparticles and its application in biosensing, belonging to the technical field of nano-biosensing. Background technique [0002] Carbon-based nanomaterials, including carbon nanotubes and graphene, hold great promise in electronic, chemical, and biological applications due to their excellent thermal, electronic, and mechanical properties. These nanostructures have been shown to act as "nanoscaffolds" for oligonucleotides and "nanoquenchers" for dyes. Therefore, sensors based on carbon-based nanomaterials are often used for fluorescence detection of nucleic acids, proteins, small molecules, and metal ions. Although these nanomaterials show clear advantages in biosensor design, the hydrophobicity and large specific surface area of ​​graphite create strong π-π stacking interactions with the adsorption of oligonucleotides, resulting in a high energy barrier that eliminates the Possibility to ob...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64C12Q1/68
CPCG01N21/6428C12Q1/68G01N2021/6432
Inventor 刘金华邹昌鹏张承武
Owner NANJING UNIV OF TECH
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