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Cordyceps militaris mycelium culture method for improving cordycepin and adenosine content and application thereof

A technology for cultivating Cordyceps militaris mycelium and mycelium, applied in the fields of application, botany equipment and methods, cultivation, etc., can solve the problems of complex medium formula, neglect of adenosine content, cumbersome cultivation methods, etc., and save manpower Material cost, high adenosine content, easy to promote effect

Inactive Publication Date: 2021-01-08
浙江理工大学绍兴生物医药研究院有限公司 +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In CN102630490, the content of cordycepin in the mycelium was increased from the original 0.6mg / g to 2.31mg / g, but the content of adenosine, the quality index of Cordyceps sinensis, was ignored, and the formula of the medium was complicated, and the cultivation method was cumbersome. Adding nutrients also pollutes the solution

Method used

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  • Cordyceps militaris mycelium culture method for improving cordycepin and adenosine content and application thereof
  • Cordyceps militaris mycelium culture method for improving cordycepin and adenosine content and application thereof
  • Cordyceps militaris mycelium culture method for improving cordycepin and adenosine content and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] A liquid culture medium of Cordyceps militaris and preparation method thereof, comprising the steps of:

[0042] 1. Preparation of mulberry leaf corn extract

[0043] Respectively dry the mulberry leaf corn to less than 10% water content, and crush it for later use;

[0044] Weigh 10-50g / L mulberry leaf powder and 10-150g / L corn dregs, add to water and stir evenly, simmer for 10-60min (preferably 30min), cool and filter with 8-16 layers of gauze (preferably 8 layers), collect Filter the residue, take the filtrate, add water to the filtrate to the volume of the initial water added, mix well and set aside.

[0045] 2. Culture medium preparation

[0046] Weigh (quantify) each component of the medium, and the composition of the medium is: glucose 5-15g / L, potassium dihydrogen phosphate 1.2-1.5g / L, sodium dihydrogen phosphate 0.8-1.2g / L, mulberry leaf corn Extract 20-200 (the total weight of mulberry leaf powder corn dregs corresponding to the mulberry leaf corn extract) ...

Embodiment 2

[0056] Cordyceps mycelium culture

[0057] Inoculate live Cordyceps thallus (the strain is Cordyceps militaris (L) Link) in the ultra-clean bench or in a sterile state, the liquid strain, the density is controlled at 3-5 bacterial balls / mL, inoculated at a volume ratio of 1:100, Three replicates were performed for each medium. Under the condition of temperature of 22° C., keep the culture in the dark for 24 hours, and then continue to culture in the dark at 22° C. and 170 rpm for 48-72 hours (preferably 72 hours). After cultivating for 2-10 hours (preferably 4 hours) under light (light intensity is controlled at 100-150 Lx), continue culturing in the dark for 24-48 hours (preferably 36 hours).

Embodiment 3

[0059] Determination of cordycepin and adenosine

[0060] The bacterial liquid was collected, boiled for 10 minutes, cooled, centrifuged at 8000r / min for 10 minutes, precipitated, dried and weighed. The contents of cordycepin and adenosine in the supernatant were measured by HPLC. The results are shown in Table 2.

[0061] Table 2 Each component medium culture mycelia weight and active ingredient content (mean value)

[0062]

[0063] It can be concluded from the experimental results that the liquid medium provided by the present invention can effectively increase the content of two active substances, cordycepin and adenosine, by culturing mycelia. Among them, the content of cordycepin is generally higher than 0.281%, the highest can reach 0.329%. The highest content of adenosine can reach more than 0.3%, and some are even higher than the content of cordycepin. Especially when the content of mulberry leaf powder in the medium is 10-25g / L and the content of corn dregs is 5...

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Abstract

The invention discloses a cordyceps militaris mycelium culture method for improving the cordycepin and adenosine content and application thereof. The cordyceps militaris mycelium culture method comprises the following steps of taking a proper amount of mulberry leaf powder and corn grit, adding water, boiling with slow fire, cooling, precipitating, filtering and taking filtrate; taking a mulberryleaf and corn extracting solution, adding glucose, peptone, monopotassium phosphate, sodium dihydrogen phosphate and magnesium sulfate, stirring for dissolving, adding water to a constant volume to arequired volume, subpackaging, sterilizing at high pressure, and cooling for later use; and inoculating live cordyceps taishanensis and a liquid strain, and performing dark-place standing for culturefor 24 h. Through application of mycelia cultured by the cordyceps militaris mycelium culture method in preparation of food, health care products, animal feed and additives and application of bacterial liquid cultured by the cordyceps militaris mycelium culture method in preparation of food, beverages, health care products, animal feed and additives, the cordycepin and adenosine content in the cordyceps militaris mycelia can be increased, and the cultured cordyceps militaris mycelia are high in cordycepin and adenosine content, simple in formula and easy to popularize.

Description

technical field [0001] The invention belongs to the field of edible fungus cultivation, and in particular relates to a method for cultivating Cordyceps militaris mycelium with increased contents of cordycepin and adenosine and an application thereof. [0002] technical background [0003] Cordyceps militaris belongs to the phylum Fungi, the class Ascomycota, the order Hypocreales, the family Clavcipitaceae, and the genus Cordyceps. Because it contains active ingredients such as cordycepin, cordycepic acid, adenosine and polysaccharides, it has the effects of anti-tumor, anti-inflammation, anti-virus, and enhancing body immunity. The medicinal value and nutritional value of fenugreek are accepted by more and more consumers, and the demand is also increasing year by year. [0004] The composition and efficacy of the artificially produced Cordyceps militaris mycelium are also similar to those of natural Cordyceps. Cordyceps mycelium contains a variety of active ingredients, suc...

Claims

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Application Information

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IPC IPC(8): A01G18/40A01G18/20A23L31/00A23L33/10A23L2/38A23K10/18
CPCA01G18/40A01G18/20A23L31/00A23L33/10A23L2/38A23K10/18A23V2002/00A23V2200/30
Inventor 陈剑清盖其静虞淼许志良
Owner 浙江理工大学绍兴生物医药研究院有限公司
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