Hermetia illucens compound amino acid fertilizer and preparation method thereof

A technology of compound amino acids and black soldier flies, which is applied to liquid fertilizers, organic fertilizers, fertilizer mixtures, etc., can solve the problems of easy occurrence of undecomposed living bodies, low content of hydrolyzed amino acids, slow protein hydrolysis, etc. The effect of high rate and short fermentation period

Active Publication Date: 2021-01-08
HAISENYUAN BIOTECHNOLOGY CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, due to the rapid reproduction of black soldier flies, the current process of using black soldier flies as high-protein substances and using microbial fermentation technology to obtain amino a

Method used

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  • Hermetia illucens compound amino acid fertilizer and preparation method thereof
  • Hermetia illucens compound amino acid fertilizer and preparation method thereof
  • Hermetia illucens compound amino acid fertilizer and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Embodiment 1-a kind of preparation method of black soldier fly compound amino acid fertilizer comprises the steps:

[0035] (1) Black soldier fly pretreatment: crush the sun-dried black soldier fly body, dry it to a moisture content of ≤10%, and mix it with dried grape skin powder at a mass ratio of 50:1 under hot air at 40°C , to obtain the pretreated composite black soldier fly powder;

[0036] (2) Preparation of seed liquid: inoculate Bacillus amyloliquefaciens Bt521 into the seed medium, and ferment and culture at 32°C and 100 rpm for 48 hours to obtain the seed liquid; the formula of the seed medium is as follows: each liter of water includes Peptone 8g, yeast extract 4g and sodium chloride 9g;

[0037](3) Preparation of fermentation medium: the pretreated composite black soldier fly powder is added to the fermentation medium according to the addition amount of 60% by mass to obtain a fermentation medium added with composite black soldier fly powder; The formula ...

Embodiment 2

[0039] Embodiment 2-a kind of preparation method of black soldier fly compound amino acid fertilizer comprises the steps:

[0040] (1) Black soldier fly pretreatment: crush the sun-dried black soldier fly body, dry it to a moisture content of ≤10%, and mix it with dried grape skin powder at a mass ratio of 80:1 under hot air at 50°C , to obtain the pretreated composite black soldier fly powder;

[0041] (2) Preparation of seed liquid: inoculate Bacillus amyloliquefaciens Bt521 into the seed medium, and ferment and culture at 35°C and 120rpm for 54 hours to obtain the seed liquid; the formula of the seed medium is as follows: each liter of water includes Peptone 12g, yeast extract 5g and sodium chloride 11g;

[0042] (3) Preparation of fermentation medium: adding the pretreated compound black soldier fly powder into the fermentation medium according to the addition amount of 50% by mass to obtain a fermentation medium added with compound black soldier fly powder; The formula ...

Embodiment 3

[0044] Embodiment 3-a kind of preparation method of black soldier fly compound amino acid fertilizer comprises the steps:

[0045] (1) Black soldier fly pretreatment: crush the sun-dried black soldier fly body, dry it to a moisture content of ≤10%, and mix it with dried grape skin powder at a mass ratio of 60:1 under hot air at 45°C , to obtain the pretreated composite black soldier fly powder;

[0046] (2) Preparation of seed liquid: inoculate Bacillus amyloliquefaciens Bt521 into the seed medium, and ferment and culture at 33°C and 110rpm for 54 hours to obtain the seed liquid; the formula of the seed medium is as follows: each liter of water includes Peptone 10g, yeast extract 4.5g and sodium chloride 10g

[0047] (3) Preparation of fermentation medium: the pretreated composite black soldier fly powder is added to the fermentation medium according to the addition amount of 60% by mass to obtain a fermentation medium added with composite black soldier fly powder; The formu...

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Abstract

The invention provides a hermetia illucens compound amino acid fertilizer and a preparation method thereof.The preparation method comprises the steps that 1, smashing and drying dried hermetia illucens bodies, and stirring and mixing obtained powder and dried grape skin powder residues under hot air according to the mass ratio of (50-80): 1; (2) inoculating bacillus amyloliquefaciens Bt521 into aseed culture medium, and carrying out fermentation, so as to obtain a seed solution; (3) adding 50-60% of an obtained composite hermetia illucens powder into a fermentation medium; (4) inoculating 1-3% of the seed solution to the fermentation medium, and carrying out oscillation fermentation at 26-30 DEG C and 150-250 rpm for 72-78 h to obtain the hermetia illucens compound amino acid fertilizer;the prepared compound amino acid fertilizer has the advantages that the raw materials are rich in protein, the degradation rate of hermetia illucens is high, the fermentation period is short, the varieties of amino acids are rich, and the content of the amino acids can reach 0.32 mg/mL.

Description

technical field [0001] The invention relates to the technical field of microbial fermentation engineering, in particular to a black soldier fly compound amino acid fertilizer and a preparation method thereof. Background technique [0002] Amino acids can promote the growth of plant roots, strong seedlings and plants, enhance the photosynthetic function of leaves and the resistance of crops to diseases and insect pests, and have a significant effect on increasing production of various crops. However, the content of amino acids in natural soil is low, and the intake of amino acids in the soil by microorganisms and soil animals in the soil makes it difficult for plants to gain an advantage in the process of competing for amino acids in the soil. Therefore, many existing crops often need artificial fertilization to supplement the amino acids needed for crop growth. In the process of artificial fertilization, in order to make fertilizers efficient and pollution-free, high-protei...

Claims

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Application Information

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IPC IPC(8): C05G1/00C05G5/20C05F17/20C05C11/00
CPCC05D9/00C05F17/20C05G5/20C05C11/00C05F1/00C05F11/08Y02P20/133
Inventor 张文飞赵新晨高雨赵梦秋王澜馨何佳利
Owner HAISENYUAN BIOTECHNOLOGY CO LTD
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