Hermetia illucens compound amino acid fertilizer and preparation method thereof
A technology of compound amino acids and black soldier flies, which is applied to liquid fertilizers, organic fertilizers, fertilizer mixtures, etc., can solve the problems of easy occurrence of undecomposed living bodies, low content of hydrolyzed amino acids, slow protein hydrolysis, etc. The effect of high rate and short fermentation period
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Embodiment 1
[0034] Embodiment 1-a kind of preparation method of black soldier fly compound amino acid fertilizer comprises the steps:
[0035] (1) Black soldier fly pretreatment: crush the sun-dried black soldier fly body, dry it to a moisture content of ≤10%, and mix it with dried grape skin powder at a mass ratio of 50:1 under hot air at 40°C , to obtain the pretreated composite black soldier fly powder;
[0036] (2) Preparation of seed liquid: inoculate Bacillus amyloliquefaciens Bt521 into the seed medium, and ferment and culture at 32°C and 100 rpm for 48 hours to obtain the seed liquid; the formula of the seed medium is as follows: each liter of water includes Peptone 8g, yeast extract 4g and sodium chloride 9g;
[0037](3) Preparation of fermentation medium: the pretreated composite black soldier fly powder is added to the fermentation medium according to the addition amount of 60% by mass to obtain a fermentation medium added with composite black soldier fly powder; The formula ...
Embodiment 2
[0039] Embodiment 2-a kind of preparation method of black soldier fly compound amino acid fertilizer comprises the steps:
[0040] (1) Black soldier fly pretreatment: crush the sun-dried black soldier fly body, dry it to a moisture content of ≤10%, and mix it with dried grape skin powder at a mass ratio of 80:1 under hot air at 50°C , to obtain the pretreated composite black soldier fly powder;
[0041] (2) Preparation of seed liquid: inoculate Bacillus amyloliquefaciens Bt521 into the seed medium, and ferment and culture at 35°C and 120rpm for 54 hours to obtain the seed liquid; the formula of the seed medium is as follows: each liter of water includes Peptone 12g, yeast extract 5g and sodium chloride 11g;
[0042] (3) Preparation of fermentation medium: adding the pretreated compound black soldier fly powder into the fermentation medium according to the addition amount of 50% by mass to obtain a fermentation medium added with compound black soldier fly powder; The formula ...
Embodiment 3
[0044] Embodiment 3-a kind of preparation method of black soldier fly compound amino acid fertilizer comprises the steps:
[0045] (1) Black soldier fly pretreatment: crush the sun-dried black soldier fly body, dry it to a moisture content of ≤10%, and mix it with dried grape skin powder at a mass ratio of 60:1 under hot air at 45°C , to obtain the pretreated composite black soldier fly powder;
[0046] (2) Preparation of seed liquid: inoculate Bacillus amyloliquefaciens Bt521 into the seed medium, and ferment and culture at 33°C and 110rpm for 54 hours to obtain the seed liquid; the formula of the seed medium is as follows: each liter of water includes Peptone 10g, yeast extract 4.5g and sodium chloride 10g
[0047] (3) Preparation of fermentation medium: the pretreated composite black soldier fly powder is added to the fermentation medium according to the addition amount of 60% by mass to obtain a fermentation medium added with composite black soldier fly powder; The formu...
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