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Tissue culture and rapid propagation method for firmiana major

A technique for rapid propagation of sycamore yunnanensis and tissue culture, which is applied in horticultural methods, botany equipment and methods, horticulture, etc., can solve the problems of reporting, lack of biotechnology of sycamore yunnanensis, etc., so as to improve the reproduction coefficient, maintain genetic stability and Consistency, easy seedling effect

Active Publication Date: 2021-01-22
KUNMING INST OF BOTANY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, the prior art does not have reports on tissue culture and rapid propagation of Wutong yunnanensis and related biotechnology aspects

Method used

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  • Tissue culture and rapid propagation method for firmiana major
  • Tissue culture and rapid propagation method for firmiana major
  • Tissue culture and rapid propagation method for firmiana major

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0051] Induction and Differentiation Media Screening Methods

[0052] Take the young terminal buds and side buds of sycamore yunnanensis as explants, soak them in 1% soapy water for 10 minutes, rinse them with running water, put them on the ultra-clean table, cut them into stems with single nodes, and use 75% Disinfect with alcohol for 10 seconds, rinse with sterile water for 3 times, disinfect the surface with 0.1% mercuric chloride solution for 8 minutes, wash with sterile water for 3 to 5 times, and inoculate in the prepared sycamore yunnanensis induction medium, 1 node per bottle.

[0053] Inoculate the sterilized stem segments in MS+0.1mg / L 6-BA+0.5mg / L IAA, MS+0.5mg / L 6-BA+0.5mg / L IAA, MS+1mg / L 6-BA +0.5mg / L IAA, MS+1.5mg / L 6-BA+0.5mg / L IAA, MS+2mg / L6-BA+0.5mg / L IAA medium, and the medium also includes 30g / L sucrose, Agar 5g / L, pH 5.8. The light time is 10 hours a day, the light intensity is 1500 Lux, the temperature is 26±3°C, and the culture period is 60 days to obta...

Embodiment 2

[0058] The nodal buds obtained in Example 1 were inoculated onto the proliferation and subculture medium, and the MS medium containing sucrose 30g / L+agar 5g / L was used as the basic medium for proliferation and subculture hormone screening, and the cytokinins were 6-BA, the concentration range is 0.1mg / L, 0.2mg / L, 0.5mg / L, 0.8mg / L, 1.0mg / L, 1.2mg / L (6 concentration gradients), auxin IAA, the concentration is 0.5mg / L, using the uniform design method. The light time is 10 hours per day, the light intensity is 1500 Lux, the temperature is 26±3°C, and the culture period is 60 days to obtain the breeding seedlings.

[0059] The results are shown in Table 2.

[0060] Table 2 Proliferation and Subculture Medium Hormone Screening

[0061]

[0062] As can be seen from Table 2, MS medium+0.8mg / L 6-BA (6-benzylpurine)+0.5mg / L IAA (indole acetic acid)+sucrose 30g / L+agar 5g / L, pH value 5.8, culture period For 60 days, the light intensity is 1500Lux, and the temperature is 23-30°C. Bu...

Embodiment 3

[0064] The proliferating seedlings prepared in Example 2 are inoculated onto strong seedlings and rooting medium. Strong seedlings and rooting medium are based on the MS medium containing sucrose 30g / L+agar 5g / L, and the concentration range of IAA is 0.1mg / L. L, 0.2mg / L, 0.3mg / L, 0.5mg / L, 0.8mg / L, 1.0mg / L (6 concentration gradients), the concentration range of IBA is 0.1mg / L, 0.2mg / L, 0.3mg / L, 0.5mg / L, 0.8mg / L, 1.0mg / L. The light time is 10 hours per day, the light intensity is 1500 Lux, the temperature is 26° C., and the culture period is 60 days to obtain rooted seedlings. Statistical rooting rate.

[0065] The results are shown in Table 3.

[0066] Table 3 Hormone Screening Results of Strong Seedling and Rooting Medium

[0067]

[0068]

[0069] As can be seen from Table 3, MS medium+0.3mg / L IBA (indole butyric acid)+0.3mg / L IAA (indole acetic acid)+30g / L sucrose+5g / L agar, pH value 5.8, culture cycle 30 days , The rooting rate reaches 91%.

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Abstract

The invention provides a tissue culture and rapid propagation method for firmiana major, and belongs to the technical field of plant tissue culture. The method is characterized in that tender terminalbuds or lateral buds of firmiana major are used as explants; through explant disinfection and a series of culture including induction, differentiation, proliferation and rooting in sequence on a specific culture medium, the problems of tissue culture, rapid propagation, introduction and domestication, protection, and development and utilization of scientific research and landscaping of firmiana major are effectively solved; and meanwhile, natural plant quantity reduction and natural vegetation area damage caused by natural disappearance, death and excessive utilization of wild resources are effectively avoided, and particularly, the method plays a positive role in maintaining the stability of special characters. According to the method, the induced differentiation rate is 78%, the propagation period is 60 days, the propagation coefficient is 3, the rooting rate is 91%, the transplanting survival rate is 95% or above, the propagation number and growth rate of firmiana major are greatlyincreased, and a technical support is provided for protection and propagation, introduction and domestication, preservation and large-scale production of the species.

Description

technical field [0001] The invention belongs to the technical field of plant tissue culture, and in particular relates to a rapid propagation method for tissue culture of sycamore yunnanensis. Background technique [0002] Yunnan sycamore (Firmiana major) belongs to Sterculiaceae (Sterculiaceae) and is a deciduous tree with a height of about 15 meters. It is a unique species in China and is mainly distributed in central and southern Yunnan, as well as in Xichang and Panzhihua in Sichuan at an altitude of 1600~ 3000 meters of mountain or slope. Yunnan sycamore is a positive tree species with lush branches and leaves, umbrella-shaped crown, beautiful tree shape, high ornamental value, and strong resistance to various toxic gases, which can be used for landscaping or planting as street trees; and because of its wild The populations are mostly distributed on the cliffs with poor soil in the hot and dry valleys of the Jinsha River, and have strong drought tolerance. Therefore, t...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 罗桂芬孙卫邦杨静刀志灵杨佳俊
Owner KUNMING INST OF BOTANY - CHINESE ACAD OF SCI
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