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Cordyceps militaris liquid culture medium and preparation method and application thereof
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A liquid medium, Cordyceps militaris technology, applied in the directions of botanical equipment and methods, application, cultivation, etc., can solve the problems of complex cultivation, only focusing on the content of a single active ingredient cordycepin, and long production cycle of fruiting bodies, etc., and achieves easy popularization. , high content of adenosine, simple formula effect
Pending Publication Date: 2021-01-26
ZHEJIANG SCI-TECH UNIV +2
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Problems solved by technology
However, the fruiting body has a long production cycle and complicated cultivation, which cannot meet people's needs
The technical method of artificially cultivating Cordyceps militarismycelium can obtain medicinally valuable Cordyceps militaris cultures in a short period of time, greatly reducing the cost of people taking Cordyceps militaris, but most of these studies focus on the output of Cordyceps militaris thalline or only focus on The single active ingredient cordycepin content, while ignoring the adenosine content which is the quality standard of Cordyceps sinensis
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Embodiment 1
[0025] A liquid culture medium of Cordyceps militaris and preparation method thereof, comprising the steps of:
[0027] Respectively dry the mulberry leaf corn to less than 10% water content, and crush it for later use;
[0028] Weigh 10-50g / L mulberry leaf powder and 10-150g / L corn dregs, add to water and stir evenly, simmer for 10-60min (preferably 30min), cool and filter with 8-16 layers of gauze (preferably 8 layers), collect Filter the residue, take the filtrate, add water to the filtrate to the volume of the initial water added, mix well and set aside.
[0029] 2. Culture medium preparation
[0030] Weigh (measure) each component of the culture medium, the composition of the culture medium is: glucose 5-15g / L, potassium dihydrogen phosphate 1.2-1.5g / L, sodium dihydrogen phosphate 0.8-1.2g / L, mulberry leaf corn Extract 20-200 (the total weight of mulberry leaf powder corn dregs corresponding to the mulberry leaf cor...
Embodiment 2
[0039] Application of a kind of Cordyceps militaris liquid culture medium
[0041] Inoculate live Cordyceps thallus (the strain is Cordyceps militaris (L) Link) in the ultra-clean bench or in a sterile state, the liquid strain, the density is controlled at 3-5 bacterial balls / mL, inoculated at a volume ratio of 1:100, Three replicates were performed for each medium. Under the condition of temperature of 22° C., the culture was kept in the dark for 24 hours, and then the culture was continued in the dark at 22° C. and 170 rpm for 60-72 hours (preferably 72 hours). After cultivating under light (light intensity controlled at 100-150Lx) for 3 hours, continue to culture in the dark for 24-48h (preferably 36h).
[0042] 2. Determination of cordycepin and adenosine
[0043] The bacterial liquid was collected, boiled for 10 minutes, cooled, centrifuged at 8000r / min for 10 minutes, precipitated, dried and weighed. The contents of cordycepin an...
Embodiment 3
[0048] The residue obtained in the process of preparing the mulberry leaf corn extract provided by the invention can also be used in the preparation of food, health food, animal feed and additives.
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Abstract
The invention discloses a cordyceps militaris liquid culture medium and a preparation method and application thereof. The cordyceps militaris liquid culture medium comprises mulberry leafcorn extract, glucose, peptone, potassium dihydrogen phosphate, sodium dihydrogen phosphate, magnesiumsulfate and water, and the preparation method comprises the following steps of adding water into silkworm chrysalis meal, boiling with slow fire for 10-60 min, cooling, precipitating, filtering with gauze, and taking a filtrate; and taking a mulberry leaf corn extracting solution, adding sugar, the peptone,the potassium dihydrogen phosphate, the sodium dihydrogen phosphate and the magnesiumsulfate, stirring for dissolving, adding water to fill to the required volume, performing sub-packaging, sterilizing at 121 DEG C and 1.1 Mpa under high pressure for 20-30 minutes, and cooling for later use. The cordyceps militaris liquid culture medium is applied to artificial culture of cordyceps militaris. Paecilomyces bepiali chen cultured by the liquid culture medium is high in cordycepin and adenosine content, and can meet the requirement of people for increasing the content of active ingredients in themycelium.
Description
technical field [0001] The invention belongs to the field of edible fungus cultivation, and in particular relates to a liquid culture medium of Cordyceps militaris and its preparation method and application. [0002] technical background [0003] Cordyceps militaris belongs to the phylum Fungi, the class Ascomycota, the order Hypocreales, the family Clavcipitaceae, and the genusCordyceps. Because it contains active ingredients such as cordycepin, cordycepic acid, adenosine and polysaccharides, it has the effects of anti-tumor, anti-inflammation, anti-virus, and enhancing body immunity. The medicinal value and nutritional value of fenugreek are accepted by more and more consumers, and the demand is also increasing year by year. [0004] Cordycepin (cordycepin), also known as 3'-deoxyadenosine, is an adenosine active substance isolated by Cuningham in 1951, and its molecular formula is C 10 h 13 N 5 0 3 . Cordycepin is one of the main active ingredients in Cordyceps mili...
Claims
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Application Information
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