Culture method for increasing content of phycoerythrin in acrochaetiaceae

A technology of phycocyanin in algae and a cultivation method, which is applied in the field of cultivation to increase the content of phycoerythrin in Acrestria, can solve problems such as wall-breaking extraction and separation procedures, achieve high phycoerythrin content and increase growth rate , the effect of increasing the content

Inactive Publication Date: 2021-01-29
NATIONAL TAIWAN OCEAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, because the algal forms of these large red algae are mostly thallus, it is difficult to extract them by breaking the wall
In addition, although these large red algae are often used as a source of agaric gum or carrageenan due to their high sugar content, the isolation procedure after extraction of phycoerythrin is also difficult due to the high sugar content

Method used

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  • Culture method for increasing content of phycoerythrin in acrochaetiaceae
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  • Culture method for increasing content of phycoerythrin in acrochaetiaceae

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Acromega culture

[0038] Take 0.5g of Acrometrix algae with a wet weight and put them into a culture container containing 800mL of seawater medium, and cultivate them in a culture environment. During the culture, pump air so that the Acrometrix algae can roll in the culture container. Wherein, the seawater culture medium is natural seawater or artificial seawater, and the ratio of the Acromegae and the seawater culture medium can be adjusted according to actual needs; wherein, the seawater culture medium contains 1600 μL of nutrient salt, and the nutrient salt and the seawater culture medium The proportion can be adjusted according to actual needs; wherein, the nutrient salt contains 70 ~ 80g / L sodium nitrate (NaNO 3 ), 4~6g / L sodium dihydrogen phosphate (NaH 2 PO 4 2H 2 O), 25~28g / L ammonium chloride (NH 4 Cl), the nutrient salt preferably comprises 75.0g / L sodium nitrate (NaNO 3 ), 5.0g / L sodium dihydrogen phosphate (NaH 2 PO 4 2H 2 O), 26.8g / L ammonium chlor...

Embodiment 2

[0040] Evaluation of the Effects of Temperature and Illumination on the Growth and Phycobiliprotein Content of Acretaxel algae

[0041] Evaluation method: use the method described in Example 1 to cultivate Acrometrix algae, and set the culture environment as a fixed culture light cycle with different culture temperatures and culture illuminance, to understand the effect of temperature and illuminance on the growth of Acregothere algae and the growth of algae. Influence of bile protein content. Specifically, the culture light cycle is fixed as 12 hours of light and 12 hours of darkness (12L:12D); the culture temperature is set to 14, 18, 22 or 26 ° C; the culture illumination is set to 20, 60 or 100 μE ; Be divided into 12 groups (as shown in Table 1) altogether, every group 3 repeats. Change the seawater every 3 days during the cultivation and weigh it to make a record. When weighing, filter it with a 200-mesh plankton net, centrifuge at 2000rpm for 5 minutes to filter out th...

Embodiment 3

[0058] Evaluation of the effect of photoperiod on the growth and phycoerythrin content of Acretaxel algae

[0059] Evaluation method: use the method described in Example 1 to cultivate Acrometrix algae, and set the culture environment to a fixed culture temperature and culture illumination with different culture light cycles to understand the effect of the light cycle on the growth of Acrometrix algae and phycobilia. Effect of protein content. Specifically, the culture temperature was fixed at 22°C; the culture illumination was fixed at 100 μE; the culture light cycle was set to 8 hours of light and 16 hours of darkness (8L:16D), and 12 hours of light and 12 hours of darkness (12L:16D). 12D) or 16 hours of light and 8 hours of darkness (16L:8D); divided into 3 groups (as shown in Table 2) in total, with 3 repetitions in each group. Change the seawater every 3 days during the cultivation and weigh it to make a record. When weighing, filter it with a 200-mesh plankton net, cent...

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Abstract

The invention provides a culture method for increasing the content of phycoerythrin in acrochaetiaceae. The culture method comprises the following steps: placing acrochaetiaceae in a seawater culturemedium containing sodium nitrate (NaNO3), sodium dihydrogen phosphate (NaH2PO4.2H2O) and ammonium chloride (NH4Cl), and conducting culturing in a culture environment at a culture temperature of 18-26DEG C under culture illuminance of 60-100 [mu]E, wherein a culture illumination source comprises blue light or green light, and the seawater culture medium is natural seawater or artificial seawater.

Description

technical field [0001] The invention relates to the technical field of cultivation of acrometrix, in particular to a cultivation method for increasing the content of phycoerythrin in acromona. Background technique [0002] Phycobiliprotein (Phycobiliprotein) is a water-soluble fluorescent protein present in algae such as red algae, green algae, cyanobacteria, and cryptophyta. Phycobiliproteins can be divided into Phycoerythrin (Phycoerythrin, PE), Phycocyanin (Phycocyanin, PC) and Paraphycocyanin (Allophycocyanin, APC). Development and research in various fields, including health care, chemicals and biological agents, oncology drugs, in vitro diagnostics, research reagents, especially reagents for flow cytometry, etc. However, due to the high production technology threshold and cost of phycoerythrin, resulting in its high price, this related application cannot be scaled up in the industry, so that the market application of phycoerythrin is still in the growth stage to be de...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/12C12R1/89
CPCC12N1/12
Inventor 李孟洲张富杰叶翰扬
Owner NATIONAL TAIWAN OCEAN UNIVERSITY
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