Preparation method and application of Chinese breast milk triglyceride substitute synthesized by enzymic method

A technology of triglyceride and enzymatic synthesis, applied in dairy products, applications, milk preparations and other directions, can solve problems such as nutrient deficiency, and achieve the effect of increasing added value, improving the composition and structure of fatty acids, and saving the amount of enzymes

Pending Publication Date: 2021-02-05
NANCHANG UNIV
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  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] The nutritional needs of infants and young children are relatively special, and it is a critical period for growth and development in life. The quantity and quality of various nutrients intake during this critical period will have a major impact on future growth. The lack and insufficiency of key nutrients, will have irreversible consequences

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  • Preparation method and application of Chinese breast milk triglyceride substitute synthesized by enzymic method
  • Preparation method and application of Chinese breast milk triglyceride substitute synthesized by enzymic method
  • Preparation method and application of Chinese breast milk triglyceride substitute synthesized by enzymic method

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preparation example Construction

[0043] The present invention is a preparation method for enzymatically synthesizing a Chinese breast milk triglyceride substitute, the preparation method comprising the following steps:

[0044] S1. Palm stearin is catalyzed by non-directional lipase to prepare palm stearin with high sn-2 palmitic acid triglyceride content;

[0045] S2. Select safflower seed oil, tea oil, perilla seed oil, lauric acid, and myristic acid according to the formula quantity, and mix them with palm stearin with high sn-2 palmitic acid triglyceride content obtained in S1 to prepare mixed oil , add lipase, preferably sn-1,3-position directed lipase enzymatic hydrolysis catalysis;

[0046] S3. Testing and evaluation: The tested enzymatically synthesized Chinese breast milk triglyceride substitutes were similar to Chinese breast milk in terms of total fatty acid composition, sn-2 fatty acid composition, and triglyceride configuration.

[0047] The enzymolysis catalytic process in step S2 is specifical...

Embodiment 1

[0069] A certain amount of palm stearin was added into a 250mL round bottom flask, the amount of non-directional lipase added was 6%, the reaction temperature was controlled at 79°C, and the reaction time was 13h to obtain sn-2 palmitic acid triglyceride. Weigh 10gsn-2 palmitic triglyceride in a reaction bottle, add safflower seed oil, tea oil, perilla seed oil, lauric acid and myristic acid, mix with 1:6 molar ratio, add 8wt% Lipozyme RMIM lipase (based on the total mass of the substrate), the reaction bottle was filled with nitrogen and sealed, and reacted in a water bath at 60° C. with a stirring rate of 200 rpm for 8 h, and performed three times in parallel. The fatty acid composition, distribution and triglyceride configuration of the obtained product triglycerides are shown in Table 4 below.

[0070] The obtained product is mixed with 65% desalted whey powder, 20% maltodextrin, 0.5% vitamin and 0.5% mineral matter, and is spray-dried to prepare dietary food for infants a...

Embodiment 2

[0075] Weigh 400g sn-2 palmitic acid triglyceride in the reaction bottle, and add safflower seed oil, tea oil, perilla seed oil, lauric acid and myristic acid, mix with 1: 6 molar ratio, add 8wt wherein % Lipozyme TL IM lipase (based on the total mass of the substrate), the reaction bottle was filled with nitrogen and sealed, and reacted in a water bath at 60° C. with a stirring rate of 200 rpm for 8 hours, and performed three times in parallel. The fatty acid composition, distribution and triglyceride configuration of the obtained product triglycerides are shown in Table 5 below.

[0076] The resulting product is mixed with 65% desalted whey powder, 20% maltodextrin, 0.5% fructooligosaccharides, 1% galactooligosaccharides, 0.5% vitamins, 0.5% minerals, and spray-dried to prepare dietary food for infants and young children. Constipation, intestinal obstruction, gastroenteritis, and abdominal pain in infants and young children.

[0077] Table 5 Fatty acid composition and distr...

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Abstract

The invention discloses a preparation method and application of a Chinese breast milk triglyceride substitute synthesized by an enzymic method. According to the substitute, vegetable oil is mainly used as a raw material to simulate a structure of Chinese breast milk triglyceride, and the vegetable oil raw material comprises palm stearin, safflower seed oil, tea oil, perilla seed oil, lauric acid and myristic acid. The preparation method comprises the following steps: catalyzing 52-degree palm stearin with non-directional lipase, mixing the palm stearin with oils, and enzymatically hydrolyzingwith sn-1,3 directional lipase. The substitute is rich in linoleic acid, has a low ratio of free fatty acids, fully simulates fatty acid composition, distribution and triglyceride configuration of Chinese breast milk, and is an excellent substitute for Chinese breast milk triglyceride. The preparation method is scientific, and can increase output in the same time. The raw materials are abundant and easily available. Compared with selection of animal fats, especially lard, the substitute avoids promotion obstacles caused by religious taboo of lard raw materials, and further has characteristicsof stable storage and wide range of use.

Description

technical field [0001] The invention relates to the technical field of enzymatic synthesis of breast milk substitutes, in particular to a preparation method and application of enzymatic synthesis of Chinese breast milk triglyceride substitutes. Background technique [0002] The nutritional needs of infants and young children are relatively special, and it is a critical period for growth and development in life. The quantity and quality of various nutrients intake during this critical period will have a major impact on future growth. The lack and insufficiency of key nutrients, will have irreversible consequences. Therefore, it is of great practical significance to provide healthy and reasonable infant formula milk powder for the majority of infants. [0003] Numerous studies have shown that breast milk is beneficial for the baby's gastrointestinal function, growth, nervous system development and immune system development and reduces the risk of infectious diseases. Human m...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23C9/152
CPCA23C9/152A23C9/1528
Inventor 李静邓泽元陈小露张兵郑溜丰贺杨正
Owner NANCHANG UNIV
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