Fibroin micro-nano fiber microsphere as well as preparation method and application thereof

A technology of micro-nano fiber and silk fibroin, applied in biochemical equipment and methods, rayon manufacturing, general cultivation methods, etc., can solve the problem of residual specific surface area of ​​harmful chemical reagents, poor mechanical properties of silk fibroin microspheres, and difficulty in controlling particle size To achieve the effect of optimizing hole shape stability, excellent mechanical properties and eliminating internal stress

Active Publication Date: 2021-02-05
WUHAN TEXTILE UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, most silk fibroin microspheres prepared from regenerated silk fibroin have problems such as poor mechanical properties, difficulty in controlling particle size, residual harmful chemical reagents, and low specific surface area.

Method used

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  • Fibroin micro-nano fiber microsphere as well as preparation method and application thereof
  • Fibroin micro-nano fiber microsphere as well as preparation method and application thereof
  • Fibroin micro-nano fiber microsphere as well as preparation method and application thereof

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Experimental program
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preparation example Construction

[0037] The invention provides a method for preparing silk fibroin micronano fiber microspheres, comprising the following steps:

[0038] S1, prepare the silk fibroin micro-nano fiber suspension for standby;

[0039] S2, preparing the polysaccharide solution for subsequent use;

[0040] S3. Mix the silk fibroin micro-nanofiber suspension prepared in step S1 with the polysaccharide solution prepared in step S2 according to the preset solute mass ratio, and mix thoroughly to obtain a mixed solution; then add the mixed solution to the mixed solution Add morpholineethanesulfonic acid, N-hydroxysuccinimide and 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride successively to obtain the reaction solution after fully reacting;

[0041] S4. Inject the reaction solution obtained in step S3 into a push injection mold, push out the reaction solution by electrostatic spraying, collect it with liquid nitrogen, and obtain silk fibroin micro-nanofiber microspheres after freeze-dryi...

Embodiment 1

[0050] This embodiment provides a method for preparing silk fibroin micro-nano fiber microspheres, comprising the following steps:

[0051] S1. Preparation of silk fibroin micronanofiber suspension

[0052] Calcium chloride, ethanol and water were mixed according to the molar ratio of 1:2:8 to prepare a ternary mixed solution, and then the degummed silk was placed in the ternary mixed solution according to the bath ratio of 1:250g / mL. Treat at ℃ for 6 hours; then add water to the treated silk fiber and place it in a physical fiber splitter with a power of 1000W. After treating for 15 minutes, collect the resulting solution, filter and dry it to make silk micro-nano fiber suspension solution and stored at 4°C for later use.

[0053] S2. Preparation of silk fibroin micro-nano fiber microspheres

[0054] Take 30mL of the silk fibroin micro-nanofiber suspension with a concentration of 0.3wt% prepared in step S1, fully stir it and inject it into the injection mold, form the micro...

Embodiment 2

[0056] This embodiment provides a method for preparing silk fibroin micro-nano fiber microspheres, comprising the following steps:

[0057] S1. Preparation of silk fibroin micronanofiber suspension

[0058] This step is the same as that in Embodiment 1, and will not be repeated here.

[0059] S2, preparing polysaccharide solution

[0060] Take 0.5 g of hyaluronic acid powder, add it to 50 mL of deionized water, and stir slowly for 6 hours to obtain a hyaluronic acid solution with a mass fraction of 1 wt%, which is sealed and stored at 4°C as a polysaccharide solution for later use.

[0061] S3. Preparation of silk fibroin micro-nano fiber microspheres

[0062] Mix the silk fibroin micro-nanofiber suspension prepared in step S1 with the polysaccharide solution prepared in step S2 at a solute mass ratio of 99:1, and stir thoroughly to obtain a mixed solution in which the mass percentage of the total solute is 0.3 wt%. Add predetermined amount of morpholinoethanesulfonic acid,...

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Abstract

The invention provides a fibroin micro-nano fiber microsphere as well as a preparation method and application thereof. According to the method, a CaCl2 / ethanol / H2O mixed solution is used for heat andhumidity treatment on silk fibroin, and silk fibroin micro-nano fiber suspension liquid is prepared through physical and mechanical fiber separation effect; and the prepared fibroin micro-nano fiber suspension is blended with a polysaccharide solution, then a cross-linking reaction is conducted, and electrostatic spraying and freeze drying treatment are performed to obtain the fibroin micro-nano fiber microsphere. By means of the above mode, fibroin micro-nano fibers can be prepared while excellent mechanical properties of fibroin fibril are reserved, and covalent bonds are formed between thefibroin micro-nano fibers and polysaccharide molecules through a crosslinking reaction, so the water absorption rate and the morphological stability of the prepared fibroin micro-nano fiber microsphere are greatly improved, and the microsphere is allowed to have a large specific surface area and rich pore structures, thereby faciliating adhesion and proliferation of cells on the surface of the microsphere. The whole preparation process of the microsphere is simple, easy to regulate and control and low in cost, and has higher application value.

Description

technical field [0001] The invention relates to the technical field of biomaterials, in particular to a silk fibroin micro-nano fiber microsphere and a preparation method and application thereof. Background technique [0002] Polymer microspheres refer to polymer materials or polymer composite materials with diameters ranging from nanometers to micrometers and spherical shapes. Polymer microspheres with different particle sizes and shapes have different functions, and are widely used as carriers for drug sustained release or delivery in the biomedical field. At present, there are many methods of preparing microspheres at home and abroad. Different preparation methods can obtain microspheres ranging from tens of nanometers to hundreds of microns, and the properties and applications of various types of microspheres are also different. For example, drug loading How to improve the existing preparation method so that the prepared microspheres have the desired performance is stil...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01J20/24B01J20/28B01J20/30D01F4/02D01F8/02D01F8/18D01F1/10C12N5/00
CPCB01J20/046B01J20/22B01J20/24B01J20/28021D01F4/02D01F8/02D01F8/18D01F1/10C12N5/0075C12N2531/00C12N2533/56C12N2533/80
Inventor 张强范红豆闫书芹黄傲胡展翱尤仁传李秀芳罗祖维
Owner WUHAN TEXTILE UNIV
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