A kind of biocontrol bacterium bc04 and its bacterial agent, bacterial agent preparation method and application
A technology of biocontrol bacteria and bacterial agents, applied in the field of microbial plant protection, to achieve good control effect, convenient field application, and simple preparation method
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Embodiment 1
[0023] Example 1 The research of biocontrol bacteria BC04 on the degradative effect of Pythium teratiflora
[0024] Cultivate the sporangia of Pythium malatus under the condition of CMA medium (corn agar medium), make it germinate, obtain zoospores, and quantify it to 2.0×10 5 cfu / mL, spare. Pick a single colony of biocontrol bacteria BC04 and inoculate it into LB liquid medium to obtain a culture medium, and use LB liquid medium to adjust the concentration of the culture medium to 2.0×10 8 cfu / mL. Set up 6 groups of treatments: clean water treatment group, culture supernatant group, bacterial suspension (bacteria obtained by centrifugation of the culture medium were resuspended in an equal volume of LB liquid medium) group, 10-fold diluted culture medium group, and 50-fold Diluted culture solution group and 100-fold diluted culture solution group were added to the quantitative spore liquid of Pythium terogenes in equal amounts to study its degradation effect on Pythium terg...
Embodiment 2
[0029] Embodiment 2 A kind of bacterial agent containing biocontrol bacteria BC04
[0030] A bacterium agent containing biocontrol bacteria BC04, said biocontrol bacteria BC04 has been preserved in the General Microbiology Center (CGMCC) of China Microbiological Culture Collection Management Committee, and the preservation address is No. 3, No. 1, Beichen West Road, Chaoyang District, Beijing, The preservation number is CGMCC NO.20907, the preservation date is October 19, 2020, the classification is named Bacillus cereus, and the dosage form of the bacterial agent is wettable powder;
[0031] The preparation method of bacterial agent comprises the steps:
[0032] (1) Inoculate the BC04 strain of biocontrol bacteria stored at -80°C into LB solid medium, and culture it upside down at 30°C±1°C for 16 hours, pick a single colony, inoculate the single colony in LB liquid medium, and incubate Cultivate at ±1°C for 18 hours to the logarithmic growth phase to obtain seed liquid for f...
Embodiment 3
[0038] Application of embodiment 3 bacterial agents in spraying soil
[0039] The test is divided into a biocontrol bacterium BC04 treatment group and a control group, and the test method is that every liter of nutrient soil (the nutrient soil is purchased from Piedman Company, the formula of the nutrient soil: organic matter content ≥ 45%, humic acid content ≥ 15%, PH value: 5.5 ~ 6.8, free water content ≤ 20%) add 10g / L bacterial agent (or 5g / L bacterial agent, 2g / L bacterial agent) and 1.0g Pythium malosterni mycelium (including zoospores) and mix , as the cultivation substrate after fully mixing; control group: add 1.0g Pythium teratiflora thalline (containing zoospores) to every liter of nutrient soil and mix it as the cultivation substrate, sow the above-mentioned treatment group and the control group seeds according to the conventional method and Routine management of corn, pepper and tomato, 30 plants in each group, repeated 3 times, observed and counted the incidence ...
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