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Method for simultaneously detecting eight ganoderic acids in ganoderma lucidum mycelia

A technology of Ganoderma lucidum mycelium and Ganoderma lucidum acid, which is applied in measuring devices, instruments, scientific instruments, etc., can solve the problems of inability to distinguish Ganoderma lucidum acid and affect the results of determination, and achieve high sensitivity, high accuracy, and strong specificity. Effect

Active Publication Date: 2021-02-09
SHANGHAI ACAD OF AGRI SCI
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Problems solved by technology

This is because there are not only triterpenoids such as Ganoderma acid in the mycelia of Ganoderma lucidum, but also various sterols such as ergosterol and beer sterol, and unsaturated fatty acids such as linoleic acid and palmitic acid. When analyzed by spectrophotometry, These substances can all react with chromogenic agents such as vanillin-glacial acetic acid and perchloric acid, and affect the results of the determination.
However, the HPLC-UV detection method also has certain defects, because this method can only be used to characterize the ganoderma acid compounds through the retention time, because many ganoderma acids in the mycelia have similar structures, resulting in similar polarity, and the retention time alone cannot be used to identify the ganoderma acid compounds Distinguish between similar ganoderma acids

Method used

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  • Method for simultaneously detecting eight ganoderic acids in ganoderma lucidum mycelia
  • Method for simultaneously detecting eight ganoderic acids in ganoderma lucidum mycelia
  • Method for simultaneously detecting eight ganoderic acids in ganoderma lucidum mycelia

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Embodiment 1

[0046] The establishment of embodiment 1 detection method

[0047] 1. The pretreatment step of Ganoderma lucidum mycelium: add 0.50 g of dry Ganoderma lucidum mycelium (Ganoderma lingzhi and Ganoderma lucidum) to 15 mL of methanol according to the ratio of material to liquid 1:30 (weight to volume ratio, weight g: volume ml), Ultrasonic extraction for 60 minutes, the supernatant was filtered with an organic phase microporous membrane with a pore size of 0.20 μm, and then diluted 100 times with mass spectrometry-grade methanol to obtain the sample solution to be tested; preparation of the mixed standard solution: take the standard of eight ganoderma acid compounds Products, mixed standard solutions with a concentration of 25 μg / mL (the concentration of each standard product is 25 μg / mL) were prepared with mass spectrometry grade methanol, and diluted step by step with mass spectrometry grade methanol to 10 μg / mL, 5 μg / mL, 2 μg / mL mL, 1μg / mL, 500ng / mL, 200ng / mL, 100ng / mL, 50ng / m...

Embodiment 2

[0065] Methodological verification and result detection: methodological verification refers to the laboratory quality control specification food physical and chemical testing standards and the relevant provisions of the Pharmacopoeia.

[0066] 1 Precision: Take the mixed standard working solution and repeat the injection 6 times in the same day, calculate the concentrations of the 8 kinds of ganoderma acids obtained from the 6 experiments according to the standard curve, and calculate the intra-day precision. The standard working solution was taken for three consecutive days, and samples were injected twice a day, and the day-to-day precision was calculated based on the results of 6 experiments. The results showed that the RSDs of the intraday precision determination results of the eight ganoderma acids were 2.30%, 4.00%, 2.60%, 2.10%, 2.00%, 1.70%, 2.30%, 1.20% respectively; the RSDs of the intraday precision determination results were respectively 5.90%, 6.40%, 5.80%, 4.80%,...

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Abstract

The invention provides a method for simultaneously detecting eight ganoderic acids in ganoderma lucidum mycelia. The method comprises the following steps: (1) pretreatment of the ganoderma lucidum mycelia; (2) ultra-high performance liquid chromatography detection; (3) acquisition of compound mass spectrum information; (4) establishment of an ultra-high performance liquid chromatography triple quadrupole mass spectrometry analysis method; and (5) data analysis. The method for simultaneously detecting the eight ganoderic acids in the ganoderma lucidum mycelia can be used for simultaneously carrying out accurate qualitative and quantitative analysis on eight specific ganoderic acid compounds in the ganoderma lucidum fermentation mycelia, is high in accuracy, high in sensitivity and strong inspecificity, and is suitable for detecting and analyzing the ganoderic acid compounds in the ganoderma lucidum mycelia, so an accurate and reliable detection and analysis method is provided for related research and products of ganoderma lucidum mycelia.

Description

technical field [0001] The invention relates to the field of detection and analysis of effective components of medicinal fungi, in particular to a method for simultaneously detecting eight kinds of ganoderma acids in ganoderma mycelia. Background technique [0002] Ganoderma sp. is a traditional medicinal fungus in my country. According to modern pharmacological research, Ganoderma is considered to have many biological activities such as anti-tumor, anti-virus, prevention and treatment of cardiovascular diseases, liver protection, anti-oxidation, and immune regulation. At present, the research on Ganoderma lucidum not only focuses on its fruiting body, but also has many reports on the research on the mycelium of Ganoderma lucidum. Scholars have isolated more than 60 compounds from the fermented mycelium of Ganoderma lucidum, mainly ganoderma acid, ganoderma alcohol, sterol and other types. Due to the different stages of growth and development, these compounds in the myceliu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/88G01N30/86
CPCG01N30/88G01N30/8679G01N2030/884G01N2030/027
Inventor 冯娜周帅张劲松岳亚文唐庆九冯杰王金艳
Owner SHANGHAI ACAD OF AGRI SCI
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