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Functionalized kit for preparing hydrogels

A kit and activator technology, applied in the field of sperm sustained-release composition, can solve problems such as lack of a large amount of research, means of preparation steps of centralized preservation system, etc.

Active Publication Date: 2021-02-12
斯博姆维透公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] To our knowledge, no substantial research has focused on the means used to simplify the preparation steps of such preservation systems

Method used

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  • Functionalized kit for preparing hydrogels
  • Functionalized kit for preparing hydrogels
  • Functionalized kit for preparing hydrogels

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0194] Example 1: Preparation of bovine spermatozoa in an alginate gel in an insemination straw

[0195] Material

[0196] The following chemicals were used: Tris hydrochloride, EDTA, NaHCO from Sigma-Aldrich (St. Luis, USA) 3 , NaCl, NaOH, glycerol (>99%) fructose, ascorbic acid and sodium citrate, carboxymethylcellulose (CMC), polyvinyl alcohol (PVA) from Sekisui Specialty Chemicals Europe S.L (Tarragona Spain), Selvol 523 / Selvol 325 . Anhydrous dextrose from Apro (Oslo, Norway). ViCality AlbaFil calcium carbonate from Brenntag Specialties (South Plainfield, USA) and sodium alginate (UP-LVG) from Novamatrix A / S (Drammen, Norway).

[0197] source of sperm

[0198] Bovine sperm in Trondheim, Norway and Stange's Store Ree's Geno device.

[0199] buffer

[0200] The following supplement solutions were used:

[0201] Supplement for the first dilution of sperm: 1.45g l -1 Tris hydrochloride glucose, 0.4g l -1 Sodium citrate, 1g l -1 Fructose, and 200ml l -1 egg yo...

Embodiment 2

[0212] Example 2: Determination of the mechanical strength of different alginate gels with immobilized sperm cells

[0213] Material

[0214] The following chemicals were used: Tris hydrochloride, EDTA, D-(+)-glucono delta-lactone, NaHCO from Sigma-Aldrich (St. Louis, USA) 3 , NaCl, glycerin (>99%), fructose and sodium citrate. Anhydrous dextrose from Apro (Oslo, Norway). Eskal 500 calcium carbonate from KSL staubtechnik GmbH (Lauingen, Germany) and sodium alginate (UPLVG and UP VLVG) from NovaMatrix, FMC BioPolymer AS (Sandvika, Norway). 0.25 ml standard insemination pipette (French micropipettor (IMV, L'Aigle, France)).

[0215] source of sperm

[0216] Bovine sperm in Trondheim, Norway and Stange's Store Ree's Geno device.

[0217] buffer

[0218] The following supplement solutions were used:

[0219] Supplement for the first dilution of sperm: 1.45g l -1 Tris hydrochloride dextrose, 0.4gl -1 Sodium citrate, 1g l -1 Fructose, and 200ml l -1 egg yolk. The...

Embodiment 3

[0225] Example 3: Coated insemination straws

[0226] Preparation of coated insemination straws

[0227] Materials: Polyvinyl alcohol Selvol 523 and Selvol 325 from Sekesui Specialty Chemicals Europe S.L. (Tarragonia, Spain), Carboxymethylcellulose (CMC) from CP Kelco: CEKOL, cellulose gum, sodium carboxymethylcellulose E466, A mixture of methylcellulose and hydroxypropylmethylcellulose Benecel MP 812W from Ashland Wilmington (Delaware USA), hypromellose Methocel K100 from Dow Chemical Company, ascorbic acid from Sigma Aldrich, AnalaR hydrochloride from VWR

[0228] 1) Coat the insemination straw by rinsing with a mixture of 13.6 μmol / mL aqueous ascorbic acid and 3% PVA (polyvinyl alcohol) aqueous solution or 5% PVA (polyvinyl alcohol).

[0229] 2) The insemination straw was coated by rinsing with a mixture (coating solution) of a 13.6 μmol / mL aqueous ascorbic acid solution and a 1.5% CMC (carboxymethylcellulose) aqueous solution. After rinsing with the coating solution,...

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Abstract

The present invention relates to a kit suitable for preparing a sustained release composition, in particular for preparing a sustained release composition comprising spermatozoa embedded within an alginate matrix.

Description

technical field [0001] The present invention relates to a kit suitable for the preparation of a sustained release composition, in particular suitable for the preparation of a sustained release composition comprising sperm embedded in an alginate matrix. Background technique [0002] Artificial insemination (AI) is a technique in which sperm are placed into the uterus or cervix of an animal by artificial means rather than through natural mating. The technique has been used since the 1940s and is now widely used as a method of mating and breeding animals to produce desired characteristics, especially of farm animals such as cattle, pigs, sheep, poultry and horses, as well as such as pedigree Desired traits for canine pets, aquatic animals and endangered species. [0003] R.H. Foote (2002), American Society of Animal Science (http: / / www.asas.org / symposia / esupp2 / Footehist.pdf) and "Reproduction in farm animals" edited by B. Hafez, E.S.E. Hafez. - No. 7 A review of the developm...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N1/02
CPCA01N1/0226A01N1/0231A01N1/0268A01N1/0263A61L31/04A01N1/02A61D19/024A61L31/12A61L31/08
Inventor E·科米斯拉德A·H·阿尔姆-克里斯蒂安森G·科林肯博格L·基拉斯W·R·格洛姆E·M·桑德鲁H·约翰森
Owner 斯博姆维透公司