Coronavirus protease activity measurement method based on fluorescence resonance energy transfer

The invention provides a coronavirus protease activity measurement method based on fluorescence resonance energy transfer. The coronavirus protease activity measurement method specifically comprises the following steps: unfreezing a coronavirus active protease sample on ice; preparing a coronavirus active protease sample solution and an FRET peptide solution by using a lysis buffer solution; adding a coronavirus active protease sample solution and an FRET peptide solution into a black half-region 96-well plate; performing mixing on a shaking table for 1 minute, sealing the experiment holes byusing a microporous plate sealing film, and performing incubating at 37 DEG C; after the temperature of the pore plate is reduced to the ambient temperature, removing the sealing film of the pore plate; reading a fluorescence intensity value when the excitation wavelength/emission wavelength is equal to 340/495nm on a luminoscope; and determining the concentration of the generated Edans labeled peptide fragment by using an Edans/Dabcyl standard curve, and calculating the enzyme specific activity by using a formula. The same experimental scheme provided by the invention can be used for detecting the activities of two virus proteases.