Super-hydrophilic coating layer for cell culture and preparation method

Abstract

The invention provides a super-hydrophilic coating layer for cell culture and a preparation method. According to the super-hydrophilic coating layer for cell culture and the preparation method, the polyvinyl alcohol-chitosan interpenetrating network super-hydrophilic coating layer is coated on a cell culture device in a spraying, dip-coating or roll-coating manner and the like, the super-hydrophilic coating layer has a durable and stable super-hydrophilic effect, and the water contact angle is smaller than 10 degrees; the prepared coating layer of the cell culture device is strong in adhesiveforce, high in transparency, resistant to ultraviolet and water soaking, good in biocompatibility, low in cytotoxicity and high in cell adherence rate.

Description

technical field [0001] The invention belongs to the field of biological materials, and in particular relates to a superhydrophilic coating for cell culture and a preparation method. Background technique [0002] With the rapid progress of modern biomedicine, stem cell culture has become a very important basic work in many research and application fields. For a long time, people have generally used polystyrene culture plates or culture bottles as tools for cell culture. Polystyrene has good light transmission performance, good strength and easy plasticity, and is non-toxic. It has become the material of choice for disposable cell culture consumables such as disposable cell culture dishes and cell culture plates. However, the surface of polystyrene is hydrophobic, so it needs to be modified to become hydrophilic before it can be suitable for cell culture. [0003] TC treatment (Tissue culture treated) is the most common surface hydrophilic modification treatment method for p...

AI Technical Summary

Problems solved by technology

Coating technology is simple to operate and does not require expensive equipment, but super-hydrophilic coatings generally have poor stability, low transparency, low adhesion, and are easily affected by ultraviolet rays and moisture.

At the same time, there are also problems such as biocompatibility, cytotoxicity, and low cell adhesion. Therefore, the preparation of super-hydrophilic surfaces based on nano-coating methods for cell culture is still a major problem.

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