Fetal cotyledon hydrogel mask and preparation method thereof
A hydrogel mask, hydrogel technology, applied in the directions of pharmaceutical formulations, cosmetic preparations, cosmetic preparations, etc., can solve the problems of increasing the popularity rate of masks, etc., to enhance skin resistance, delay skin aging, and promote The effect of skin regeneration
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[0045] The following takes goat placenta as an example.
[0046] 1. Placental cotyledon separation: healthy goat placenta was washed 3 times with water, and then washed 3 times with phosphate buffered saline pre-cooled at 4°C. First, the amnion was separated, and then the cotyledon was separated from the amniotic membrane.
[0047] 2. Cotyledons pretreatment: put the washed cotyledons in a clean container, add 10 times (1:9, V / V) distilled water, keep shaking at 4°C for 24 hours, remove residual blood and wash. Then take 1% TritonX-100PBS solution to immerse the cotyledons in it, shake at 4°C for 4 hours to decontaminate and degrease, take it out and wash it with water for injection.
[0048] Preparation of 1% TritonX-100: Mix TritonX-100 stock solution and PBS at a volume ratio of 3:7, place in a water bath at 37°C to 40°C for 3 hours to fully dissolve and mix, and prepare 30% TritonX-100 stock solution; Just before use, dilute this stock solution 30 times to make 1% TritonX...
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