Single-injection vaccine of protein antigen and preparation method of single-injection vaccine
A protein and vaccine technology, applied in the field of biomedicine, can solve problems such as the inability to achieve vaccine immunity
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[0034] The present invention also provides a method for preparing a single injection vaccine for protein antigens according to the above technical solution, comprising: 1) encapsulating the protein antigens into a carrier to obtain an antigen carrier; 2) using the antigen carrier obtained in step 1). Alternate immersion in the solution of tannic acid and high molecular polymer makes the surface of the antigen carrier form a tannic acid-high molecular polymer erodible coating, and the thickness of the erodible coating is adjusted by controlling the number of immersion times. 3) Different thicknesses obtained in the mixing step 2) can erode the coating-coated antigen carrier, and a single injection vaccine of protein antigens can be obtained.
[0035] In the present invention, the solution of the tannic acid and the high molecular polymer is preferably pH<6; the tannic acid-high molecular polymer erodible coating is preferably injected subcutaneously, and can dissociate at a cons...
Embodiment 1
[0037] Example 1 Preparation of a single injection vaccine using ovalbumin as an antigen model
[0038]The specific preparation steps are: dissolving ovalbumin into calcium chloride solution at a concentration of 1 mg / mL, adding an equal volume of sodium carbonate solution, stirring to form a calcium carbonate carrier loaded with ovalbumin, and centrifuging to collect the protein antigen carrier. Tannic acid and polyethylene glycol were respectively dissolved in phosphate buffer (pH=6) at a concentration of 1 mg / mL. Soak the calcium carbonate carrier loaded with ovalbumin alternately in tannic acid and polyethylene glycol solutions, each soaking time is 1 min, and one cycle is one layer. The control system temperature is 37°C. Repeat the cycle 20 or 40 times to obtain antigen carriers coated with different thicknesses of erodible coatings, mix with antigen carriers without erodible coatings, and dry to obtain a single-injection vaccine of ovalbumin antigen.
Embodiment 2
[0039] Embodiment 2 prepares the single injection vaccine of novel coronavirus
[0040] The specific preparation steps are: dissolving 9.8g of cetyltrimethylammonium bromide and 5g of sodium silicate in 175mL of ultrapure water, mixing and stirring, adding 17.5mL of ethyl acetate, and standing at room temperature for 5h. After aging in a water bath at 90°C for 48 hours, the product was collected by centrifugation. Finally, the product was calcined at 550°C for 10 h in an air atmosphere to obtain mesoporous silica spheres. Dissolve the novel coronavirus spike protein (His-Tag) in phosphate buffer (pH=5) at a concentration of 2 mg / mL, then add 8 mg of mesoporous silica spheres, sonicate until dispersed, and centrifuge to obtain the loaded Mesoporous silica sphere carrier for novel coronavirus characteristic protein. Tannic acid and polyethylene oxide-polypropylene oxide-polyethylene oxide copolymer (PEO-PPO-PEO) were respectively dissolved in hydrochloric acid solution (pH=3) ...
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