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Software for microfluidic systems interfacing with mass spectrometry

A fluid channel and fluid communication technology, applied in the field of chemical analysis, can solve the problem of not providing a calibration system to re-establish the Taylor cone

Pending Publication Date: 2021-03-19
INTABIO LLC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, like capillaries, these tools typically allow limited characterization of the separated analyte fractions prior to introduction to the mass spectrometer (if present)
Also, systems with capillary or microfluidic devices typically do not provide tools for calibrating the system during operation to re-establish the Taylor cone

Method used

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  • Software for microfluidic systems interfacing with mass spectrometry
  • Software for microfluidic systems interfacing with mass spectrometry
  • Software for microfluidic systems interfacing with mass spectrometry

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0137] Example 1 - Characterization of protein charge on a chip before mass spectrometry analysis

[0138] figure 1 The fabrication of the microfluidic device shown in has been described above. For operation, the device is mounted on an instrument containing a nitrogen source, a heater, a positive pressure pump (e.g., Parker, T5-1IC-03-1EEP), terminated by two platinum-iridium electrodes (e.g., Sigma-Aldrich , 357383), an electrophoresis power supply (Gamm High Voltage, MC30), a UV light source (e.g., LED, qphotonics, UVTOP280), a CCD camera (e.g., ThorLabs, 340UV-GE) and an autosampler for loading samples onto the device device. The power supply shares a common ground with the mass spectrometer. The instrument is controlled by software (eg, LabView).

[0139] Protein samples were premixed with an ampholyte pH gradient and pI markers, then placed into vials and loaded onto an autosampler. The mixture is loaded continuously from the autosampler onto the microfluidic devic...

Embodiment 2

[0146] Example 2 - Tracking the velocity of an analyte peak as it leaves the microfluidic chip and enters the mass spectrometer

[0147] For this example, Figure 7A The microfluidic channel network 100 in is fabricated in a 250 micron thick opaque cyclic olefin polymer layer. The channel 112 has a depth of 250 microns and therefore cuts all the way through the 250 micron layer. All other channels have a depth of 50 μm. Such as Figure 7B As shown, the channel layer is sandwiched between two transparent layers of a cyclic olefin polymer to fabricate a planar microfluidic device. Ports 102, 104, 106, 108 and 110 provide access to the channel network for introducing reagents from external containers and making electrical contacts. Port 102 is connected to a vacuum source, making channel 103 a waste channel, allowing other reagents to perfuse as "waste" through the channel network. Acid (1% formic acid) is perfused through port 108 into channels 109 , 112 , 114 and 103 and o...

Embodiment 3

[0153] Example 3 - Using Feedback to Adjust MS and ESI Parameters

[0154] In embodiment 3, the chip, instrument and software execute all the same procedures as in embodiment 2. In addition, if Figure 8 As shown, a second CCD camera was used to image the Taylor cone during ESI. These images were used to evaluate the quality and consistency of Taylor cones. Evaluation of images and / or totals on a mass spectrometer allows identification of ESI Taylor cone failures and diagnosis of the cause.

[0155] The formation of a Taylor cone in ESI depends on maintaining an input flow into the cone that matches the rate of fluid loss to evaporation and ESI. The size of the Taylor cone depends on the flow rate, the voltage gradient between the microfluidic device and the MS, the distance between the microfluidic device and the MS, and the subtle changes and local environment of the ESI tip of the microfluidic device.

[0156] Imaging of Taylor cones allows diagnosis of the cause of ESI...

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Abstract

Methods, devices, and systems for improving the quality of electrospray ionization mass spectrometer (ESI-MS) data are described, as are methods, devices, and systems for achieving improved correlation between chemical separation data and mass spectrometry data.

Description

[0001] cross reference [0002] This application claims the benefit of U.S. Provisional Application No. 62 / 678,265, filed May 31, 2018, and U.S. Provisional Application No. 62 / 684,090, filed June 12, 2018, both of which are incorporated herein by reference . Background technique [0003] The present disclosure relates to the field of chemical analysis, and more particularly to the separation of analytes in a mixture and their subsequent analysis by mass spectrometry (MS). Separating analyte components from more complex analyte mixtures based on their intrinsic mass and providing fractions enriched in that mass state is a key part of analytical chemistry. Simplifying complex mixtures in this way reduces the complexity of downstream analysis. However, complications can arise when attempting to combine known enrichment methods and / or devices with analytical equipment and / or techniques. [0004] For example, various approaches have been used to integrate protein sample prepara...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): H01J49/16G01N27/447G01N30/72B01L3/00
CPCG01N27/447G01N27/44713G01N27/44795H01J49/165G01N30/7266G05F1/10G01P5/22G01N30/6095G01N30/86G01N2030/8831G01N27/44791G01N30/6078G01N30/8631H01J49/147B01L3/502761B01L3/502715B01L3/502792B01L2300/0838B01L2400/0415B01L2200/0647
Inventor 艾瑞克·珍塔伦史蒂夫·拉西斯科特·迈克卢克·布斯莫滕·延森
Owner INTABIO LLC
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