Method for preparing active polypeptide from lima beans
A technology of active polypeptide and horse bean flour, which is applied to the protein composition, application, and function of food ingredients of vegetable seeds, etc., can solve the problems such as the bitterness or peculiar smell of polypeptide products, and the removal of bitter substances in polypeptide products is not considered. Conducive to large-scale preparation, good promotion and application prospects, and the effect of reducing cholesterol activity
Active Publication Date: 2021-03-23
上海健者有方健康科技发展有限公司
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Abstract
The invention discloses a method for preparing active polypeptide from lima beans, which comprises the following steps: S1, pulverizing lima beans, screening to obtain lima bean powder, adding water,uniformly mixing, inoculating activated monascus and aspergillus oryzae, fermenting at 28-35 DEG C for 2-3 days, and periodically stirring to complete one-step fermentation; secondly, preparing a fermentation medium after the one-step fermentation is finished; then inoculating activated saccharomycetes and monascus, and carrying out ventilation fermentation for 12-24 hours at the temperature of 28-30 DEG C to finish two-step fermentation; S2, after the fermentation in the step S1 is finished, adding fermentation liquor, carrying out enzymolysis under the assistance of ultrasonic waves, and carrying out enzyme deactivation and centrifugation after the enzymolysis is finished, so as to obtain enzymatic hydrolysate and precipitate; and desalting and decoloring the enzymatic hydrolysate through anion-cation exchange resin, concentrating, and carrying out vacuum freeze drying to obtain the lima bean active polypeptide product. According to the method, bitter substances in the lima bean polypeptide product can be effectively removed, so that the product is free of bitter taste or other peculiar smells and has fermentation fragrance.
Application Domain
Food ingredient as antioxidantProtein composition from vegetable seeds +2
Technology Topic
MonascusFermentation broth +11
Examples
- Experimental program(3)
Example Embodiment
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PUM
Property | Measurement | Unit |
Mesh | 40.0 | mesh |
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