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Microcapsule-embedded probiotics capable of being released at fixed points in intestinal tracts and preparation method thereof

A technology of microcapsules and probiotics, which is applied in the field of probiotics, can solve the problems such as the hydrophobic and water-proof performance of probiotics, and the reduction of the utilization rate of probiotics, so as to improve the acid and oxygen barrier effects of microcapsules and enhance the ability of fixed-point release , the effect of protecting against gastric acid erosion

Pending Publication Date: 2021-03-26
上海扬彩生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, none of the technical solutions disclosed in these two patent application documents can solve the hydrophobic and water barrier properties of probiotics in the intestinal tract, resulting in a significant reduction in the utilization of probiotics

Method used

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  • Microcapsule-embedded probiotics capable of being released at fixed points in intestinal tracts and preparation method thereof
  • Microcapsule-embedded probiotics capable of being released at fixed points in intestinal tracts and preparation method thereof
  • Microcapsule-embedded probiotics capable of being released at fixed points in intestinal tracts and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0065] The preparation of microcapsule embedding Lactobacillus casei LC2W comprises the following steps:

[0066] (1) Cultivation of probiotics: Lactobacillus casei LC2W is activated in a water bath for 30 minutes, the temperature of the water bath is 37°C, and 10% of the bacteria solution is added to the MRS medium (after sterilization) for subculture until the strain is viable number is 10 9 CFU / g or more, centrifuge 1L of the culture solution of Lactobacillus casei LC2W at 6000rpm for 10min, and collect the bacterial sediment;

[0067] (2) Preparation of W / O core material: In order to improve the survival rate and embedding rate of probiotics, the collected Lactobacillus casei LC2W colonies (10 9-10 CFU / g) is mixed with glycerol (protective agent) to form internal water phase (wherein, the consumption of glycerol is 1g lactic acid bacteria casei LC2W bacterial colony uses 12mL glycerol), and internal water phase is stirred with medium chain triglyceride (Sinopharm Chemical...

Embodiment 2

[0073] The preparation of microcapsule embedding Lactobacillus casei LC2W comprises the following steps:

[0074] (1) Cultivation of probiotics: Lactobacillus casei LC2W is activated in a water bath for 30 minutes, the temperature of the water bath is 37°C, and 10% of the bacteria solution is added to the MRS medium (after sterilization) for subculture until the strain is viable number is 10 9 CFU / g or more, centrifuge 1L of the culture solution of Lactobacillus casei LC2W at 6000rpm for 10min, and collect the bacterial sediment;

[0075] (2) Preparation of W / O core material: In order to improve the survival rate and embedding rate of probiotics, the collected colonies (10 9- 10 CFU / g) is mixed with glycerol (protective agent) to form internal water phase (wherein the consumption of glycerin is 1g lactic acid bacteria casei LC2W bacterium colony uses 18mL glycerol) mixes to form internal water phase, then with corn oil (wherein, the consumption of corn oil is 1g cheese Lact...

Embodiment 3

[0081] The preparation of microcapsule embedding Lactobacillus casei LC2W comprises the following steps:

[0082] (1) Cultivation of probiotics: Lactobacillus casei LC2W is activated in a water bath for 30 minutes, the temperature of the water bath is 37°C, and 10% of the bacteria solution is added to the MRS medium (after sterilization) for subculture until the strain is viable number is 10 9 CFU / g or more; centrifuge 1L of the culture solution of Lactobacillus casei LC2W at 6000rpm for 10min, and collect the bacterial sediment;

[0083] (2) Preparation of W / O core material: In order to improve the survival rate and embedding rate of probiotics, the collected colonies (10 9- 10 CFU / g) is mixed with bovine serum albumin (Sigma Aldrich (Shanghai) Trading Co., Ltd.) to form the inner water phase (wherein, the consumption of bovine serum albumin is that 1g of Lactobacillus casei LC2W bacterial colony uses 15g bovine serum albumin), and then Stir with soybean oil to form a W / O ...

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Abstract

The invention discloses microcapsule-embedded probiotics capable of being released at fixed points in intestinal tracts and a preparation method thereof. The preparation method comprises the followingsteps: preparing probiotics subjected to expansion culture into a W / O type core material, coating a wall material composed of whey protein and sodium alginate, and sequentially carrying out Ca<2+> primary curing, nanocrystalline cellulose coating and Ca<2+> secondary curing. The microcapsule embedded with the probiotics is good in enteric solubility, and the activity of the probiotics can still reach about 10<6> CFU / g after simulated gastrointestinal digestion.

Description

technical field [0001] The invention belongs to the technical field of probiotics, and more specifically relates to a microcapsule-embedded probiotic capable of fixed-point release in the intestinal tract and a preparation method thereof. Background technique [0002] Probiotics refer to live microbial preparations, which can promote nutrient absorption, prevent and treat intestinal-related diseases, enhance the body's immunity, and delay aging by regulating the host's mucosal and systemic immune functions and improving the host's intestinal microecological balance. According to the Japan Fermented Milk and Lactic Acid Beverage Association, the probiotic content must be at least 10 6 Only CFU / g has enough live bacteria to exert health care function. However, the resistance of probiotics to the external environment is very weak. Many studies have confirmed that the survival rate of probiotics in yogurt and fermented milk is very low. count from 10 9 CFU / g drops to 10 4 Be...

Claims

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Application Information

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IPC IPC(8): A23L33/135A23L29/00A23L29/30A23P10/30A23P20/17A61K9/50A61K47/36A61K47/42A61K47/38A61K35/741A61K35/745A61K35/747A61P1/00
CPCA23L33/135A23L29/015A23L29/045A23L29/30A23P10/30A23P20/17A61K9/5036A61K9/5042A61K9/5052A61K35/741A61K35/745A61K35/747A61P1/00A23V2002/00A23V2400/143A23V2400/125A23V2400/165A23V2400/113A23V2400/175A23V2400/321A23V2400/515A23V2400/519A23V2400/531A23V2400/169A23V2200/22A23V2200/224A23V2200/3204A23V2250/1578A23V2250/18A23V2250/5026A23V2250/54252A23V2250/5108A23V2250/1944A23V2250/511A23V2250/5436A23V2250/55A23V2250/6406A23V2300/10A23V2300/20
Inventor 张洪才姜官鑫白一菲陈斌
Owner 上海扬彩生物科技有限公司
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