Preparation method of nano liposome

Abstract

The invention discloses a preparation method of nano lipidosome. The preparation method comprises the following steps: S1, extracting maize yellow pigment; S2, weighing the maize yellow pigment and the Tween 80, and dissolving the maize yellow pigment and the Tween80 into a Na2HPO4KH2PO4 buffer solution, so as to obtain a water phase; S3, dissolving egg yolk lecithin and cholesterol in absolute ethyl alcohol to prepare an organic phase; and S4, rapidly injecting the organic phase into the water phase, performing stirring to obtain a maize yellow pigment nano liposome suspension, performing reduced pressure rotary evaporation on the suspension, and enabling residual ethanol to volatilize to obtain the maize yellow pigment nano liposome. According to the method, the maize yellow pigment is extracted from maize by adopting an ultrasonic-assisted enzyme method, so that the extraction time is shortened, the extraction is more sufficient, the maize yellow pigment nano liposome is prepared byadopting an ethanol injection method, and the stability, water solubility and in-vitro and in-vivo absorption effects of the maize yellow pigment can be improved through nanocrystallization, so thatthe bioactivity of the maize yellow pigment is fully exerted; and a nano-structure system having good stability and capable of promoting digestion and absorption is prepared.

Description

technical field [0001] The invention belongs to the technical field of nano-liposomes, in particular to a method for preparing nano-liposomes. Background technique [0002] Zeaxanthin is a mixture of oxygen-containing derivatives of several carotenoids that can be extracted from corn. It is mainly composed of zeaxanthin, lutein, cryptoxanthin and β-carotene. It can be widely used in medicine, food, Packaging and other industries. Zeaxanthin is an isoprenoid pigment, which has important physiological functions, such as inhibiting tumor growth, preventing cardiovascular and cerebrovascular diseases, enhancing human immunity and preventing age-related macular degeneration, etc. The extraction of traditional zeaxanthin is organic Solvent extraction method not only takes a long time to extract, but also has a low effective dissolution rate of zeaxanthin; liposomes are closed vesicles formed spontaneously by amphiphilic molecules dispersed in aqueous solution, protecting the cont...

AI Technical Summary

Problems solved by technology

Zeaxanthin is an isoprenoid pigment, which has important physiological functions, such as inhibiting tumor growth, preventing cardiovascular and cerebrovascular diseases, enhancing human immunity and preventing age-related macular degeneration, etc. The extraction of traditional zeaxanthin is organic Solvent extraction method not only takes a long time to extract, but also has a low effective dissolution rate of zeaxanthin; liposomes are closed vesicles formed spontaneously by amphiphilic molecules dispersed in aqueous solution, protecting the contents And sustained release, and because of the small particle size of the liposome, it is beneficial to improve the absorption and utilization rate of the core material