Aspergillus niger capable of rapidly degrading zearalenone and application of aspergillus niger

A technology of zearalenone and Aspergillus niger, applied in the field of microorganisms, can solve the problems of long action time, unfavorable preservation of raw materials, unsatisfactory degradation effect, etc., and achieve the effects of high food safety, fast binding speed and high degradation efficiency

Active Publication Date: 2021-03-26
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, although the Aspergillus niger strain currently used to degrade ZEN has a high degradation rate for ZEN degradation, it takes a long time to achieve a very high degradation rate, and the degradation effect in a short time is very unsatisfactory.
According to the previous research, a strain of Aspergillus niger that can completely degrade zearalenone was found, if according to 1×10 7 After inoculation and culture of 1-2% of the spore suspension of CFU / mL, the culture can achieve a better degradation effect on zearalenone within 3-5 days, but the long-term action time is not conducive to raw materials preservation of

Method used

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  • Aspergillus niger capable of rapidly degrading zearalenone and application of aspergillus niger
  • Aspergillus niger capable of rapidly degrading zearalenone and application of aspergillus niger
  • Aspergillus niger capable of rapidly degrading zearalenone and application of aspergillus niger

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Embodiment 1: the acquisition of mutant Aspergillus niger

[0026] Mutagenesis steps:

[0027] Mutagenesis method: use high-concentration zearalenone (ZEN) to stimulate Aspergillus niger FS-Z1 for 24 hours, and cycle 5 times, compare the degradation rate of each generation for screening, and select the one with the highest degradation rate as FS- 10-YJ.

[0028] 1. Prepare a sterile petri dish, pour PDA medium into the aseptic operating table (light the alcohol lamp in the table), the amount of the medium exceeds half of the capacity of the petri dish, and prepare 5 blank plates. After it solidifies, invert it.

[0029] 2. Take 0.25mL of the bacterial liquid (Aspergillus niger FS-Z1) in the freezing tube and put it on the PDA plate, spread it evenly with a spreader, and cultivate it in a 28°C incubator for 2-3 days.

[0030] 3. When Aspergillus niger grows on the plate, scrape the spores with a cotton swab, rinse them in sterilized normal saline, and make a suspensio...

Embodiment 2

[0039] Example 2: Degradation of ZEN by Aspergillus niger FS-10-YJ

[0040] Degrade 500ppb of ZEN toxin in PDB medium.

[0041] 1. Prepare 20mL of PDB medium in a fungal culture bottle, add ZEN toxin, and control the toxin content in the medium to 500ppb, and co-cultivate for 60h;

[0042] 2. Add the mutagenic Aspergillus niger spore suspension, and control the spore concentration in the medium to 10 3 、10 4 、10 5 、10 6 CFU / mL;

[0043] 3. Put the culture bottle into a 28°C, 180rpm fungal shaker for cultivation, take out the corresponding samples every 4 hours, extract with chloroform, and use high performance liquid chromatography to detect the toxin content.

[0044] High performance liquid chromatography conditions:

[0045] Type of HPLC: Agilent Technologies 1260infinity;

[0046] Chromatographic column: C18 column, column length: 150mm, inner diameter: 4.6mm, viscosity: 4μm;

[0047] Mobile phase: acetonitrile: water: methanol = 46:46:8;

[0048] Flow rate: 1.0mL...

Embodiment 3

[0057] Embodiment 3: the passage stability of Aspergillus niger FS-10-YJ

[0058]This mutagenesis Aspergillus niger is carried out PDA culture medium plate coating plate, subculture 5 generations, according to method in embodiment 2 (bacteria concentration is 10 6 CFU / mL, ZEN concentration is 1ppm), use Aspergillus niger FS-10-YJ to treat the ZEN solution for 36h, and measure the degradation rate after the reaction. It can be known from Table 2 that the degradation rate of the mutagenized Aspergillus niger can be stabilized above 95% after 5 generations, and the genetic stability is better.

[0059] The passage stability of table 2 Aspergillus niger FS-10-YJ

[0060] 1st generation 2nd generation 3rd generation 4th generation 5th generation ZEN degradation rate% 95.24％ 96.84％ 95.16％ 96.84％ 96.84％

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Abstract

The invention discloses aspergillus niger capable of rapidly degrading zearalenone and application of the aspergillus niger, and belongs to the field of microorganisms. According to the invention, a strain of capable of effectively degrading the zearalenone is screened out by performing capable of effectively degrading zearalenone is screened out on original aspergillus niger. The strain has highbinding speed with zearalenone and high degradation efficiency, and can degrade 95% or more of the zearalenone within 28h; a relatively high degradation effect can be achieved by using a relatively small amount of zearalenone; degradation of the same concentration of zearalenone needs 1 / 10 of the use amount of the original strain, and the strain is a food-grade strain; and the aspergillus niger can be used for degrading zearalenone in cereals such as corn and barley.

Description

technical field [0001] The invention relates to a strain of Aspergillus niger capable of rapidly degrading zearalenone and an application thereof, belonging to the field of microorganisms. Background technique [0002] Zearalenone (Zearalenone, ZEN) is a class of Fusarium mycotoxins with a wide range of contamination and estrogen-like activity. ZEN can widely pollute corn, grains and other food crops around the world, which not only brings huge economic losses to the food and feed industry, but also can accumulate in animals or humans through the food chain, causing acute poisoning and death in animals or humans. Chronic poisoning. Many studies have shown that zearalenone can not only cause human and animal reproductive system disorders, but also has strong immunotoxicity, cytotoxicity and liver toxicity, and can enter the food chain through contaminated crops, and long-term consumption of contaminated crops Food may also cause cancer, which seriously threatens the health ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14A23L5/20C12R1/685
CPCC12N1/14A23L5/28
Inventor 纪剑于坚孙秀兰张银志孙嘉笛邹东杨阳
Owner JIANGNAN UNIV
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