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Hemarthria herbage silage lactobacillus additive and preparation method thereof

A technology of lactic acid bacteria and bullbena, applied in the field of microorganisms, can solve problems such as the ineffectiveness of bullbena forage silage, and achieve the effects of improving sensory quality, fast growth rate and improving quality

Active Publication Date: 2021-03-26
CHONGQING ACAD OF ANIMAL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] However, when the lactic acid bacteria additive is used to silage Pennisetum genus forage grass, the content of liquid nitrogen is still high; in addition, the fermentation characteristics of different silage materials are not the same (CN108265016A), and the lactic acid bacteria additive adopted in this scheme is only effective on Pennisetum genus. It is effective for pasture silage, but it is not effective for pasture silage of Verbena

Method used

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  • Hemarthria herbage silage lactobacillus additive and preparation method thereof
  • Hemarthria herbage silage lactobacillus additive and preparation method thereof
  • Hemarthria herbage silage lactobacillus additive and preparation method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0034] The preparation of verbena silage lactic acid bacteria additive, concrete steps are as follows:

[0035] 1) Take "Ya'an" Verbena genus forage samples, add sterile distilled water, and dilute to 10 -1 , 10 -3 and 1 0-5 Sample dilutions of three dilutions; draw sample dilutions and apply to MRS solid medium for culture, and subculture twice continuously to obtain purified colonies;

[0036] 2) After culturing the purified colony obtained in step 1) on a lactic acid bacteria solid medium at 37° C. under constant temperature and anaerobic conditions for 24 hours, carry out the identification of lactic acid bacteria by Gram staining and catalase contact reaction, and the identified lactic acid bacteria Adding it to a liquid nutrient medium containing sterilized glycerol and storing it at -80°C to obtain isolated and purified lactic acid bacteria;

[0037] 3) After activating the isolated and purified lactic acid bacteria obtained in step 2), inoculate 10 ml of MRS liquid ...

Embodiment 2

[0048] Lactic acid bacteria additive as verbena silage additive

[0049] The lactic acid bacteria additives of the experimental group 1-3 shown in Table 3 and the bacterial liquid of the control group 1-3 were respectively inoculated into the MRS liquid medium according to the inoculum amount, and sterilized distilled water was used as a blank control to obtain a bacterial strain additive solution, and the bacterial strain live The number of bacteria is set at 1×10 6 CFU / g FW;

[0050] Take "Ya'an" Verbena flat spike samples, cut into about 20mm in length, and obtain fresh samples. Each group shown in Table 2 takes 500g of fresh samples and puts them into plastic bags (25×35cm; Aodeju, China);

[0051] Spray the added solution of the strains shown in Table 3 evenly into the pasture sample, mix evenly, pack silage in a bag, vacuum seal it, leave it at room temperature (27-37° C.) and ferment it for 60 days, then unpack it to obtain a silage sample.

[0052] Sensory evaluation...

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Abstract

The invention belongs to the technical field of microorganisms, and particularly relates to a hemarthria herbage silage lactobacillus additive. The hemarthria herbage silage lactobacillus additive comprises a component A and a component B; the component A and the component B are each any one of lactobacillus rhamnosus 753, lactobacillus salivarius 358 and lactobacillus plantarum LP694, and the component A and the component B are different; the mass ratio of the component A to the component B is (1-2): (1-2); the preservation number of the lactobacillus rhamnosus 753 is CGMCC No.18233, the preservation number of the lactobacillus salivarius 358 is CGMCCNo.18232, and the preservation number of the lactobacillus plantarum LP694 is CGMCC No.15073. The lactobacillus additive disclosed by the invention is high in growth rate and high in acid production efficiency; the sensory quality of hemarthria herbage silage can be obviously improved; the lactic acid and acetic acid content of the hemarthria herbage silage can be significantly increased, and the content of harmful acids including butyric acid and the like is reduced, so that lactobacillus becomes dominant microflora, harmful bacteriain the hemarthria herbage silage are inhibited, and the ensiling quality of herbage is improved.

Description

technical field [0001] The invention belongs to the technical field of microbes, and in particular relates to a bullweed silage lactic acid bacteria additive and a preparation method thereof. Background technique [0002] Silage refers to a storage technology or method that compresses and seals fresh plants to isolate the stored green fodder from the outside air, resulting in internal hypoxia, resulting in anaerobic fermentation. Silage helps to improve the utilization value of feed, expand the source of feed, adjust the supply period of forage, make the total nutrient harvested per unit area reach the highest value, reduce the waste of nutrients, reduce labor intensity, improve work efficiency, and treat pests and diseases. [0003] Silage is essentially lactic acid bacteria fermented feed, and natural silage is fermented by lactic acid bacteria that exist on plants in nature. However, due to the low content of lactic acid bacteria on plants in nature, they only account fo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A23K30/18C12R1/07C12R1/25
CPCC12N1/20A23K30/18A23V2400/181A23V2400/175A23V2400/169Y02P60/87
Inventor 冉启凡高武超徐远东何玮范彦蒋林峰
Owner CHONGQING ACAD OF ANIMAL SCI
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