Hemarthria herbage silage lactobacillus additive and preparation method thereof
What is Al technical title?
Al technical title is built by PatSnap Al team. It summarizes the technical point description of the patent document.
A technology of lactic acid bacteria and bullbena, applied in the field of microorganisms, can solve problems such as the ineffectiveness of bullbena forage silage, and achieve the effects of improving sensory quality, fast growth rate and improving quality
Active Publication Date: 2021-03-26
CHONGQING ACAD OF ANIMAL SCI
View PDF5 Cites 0 Cited by
Summary
Abstract
Description
Claims
Application Information
AI Technical Summary
This helps you quickly interpret patents by identifying the three key elements:
Problems solved by technology
Method used
Benefits of technology
Problems solved by technology
[0005] However, when the lactic acid bacteria additive is used to silage Pennisetum genus forage grass, the content of liquid nitrogen is still high; in addition, the fermentation characteristics of different silage materials are not the same (CN108265016A), and the lactic acid bacteria additive adopted in this scheme is only effective on Pennisetum genus. It is effective for pasture silage, but it is not effective for pasture silage of Verbena
Method used
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more
Image
Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
Click on the blue label to locate the original text in one second.
Reading with bidirectional positioning of images and text.
Smart Image
Examples
Experimental program
Comparison scheme
Effect test
Embodiment 1
[0034] The preparation of verbena silage lactic acid bacteria additive, concrete steps are as follows:
[0035] 1) Take "Ya'an" Verbena genus forage samples, add sterile distilled water, and dilute to 10 -1 , 10 -3 and 1 0-5 Sample dilutions of three dilutions; draw sample dilutions and apply to MRS solid medium for culture, and subculture twice continuously to obtain purified colonies;
[0036] 2) After culturing the purified colony obtained in step 1) on a lactic acid bacteria solid medium at 37° C. under constant temperature and anaerobic conditions for 24 hours, carry out the identification of lactic acid bacteria by Gram staining and catalase contact reaction, and the identified lactic acid bacteria Adding it to a liquid nutrient medium containing sterilized glycerol and storing it at -80°C to obtain isolated and purified lactic acid bacteria;
[0037] 3) After activating the isolated and purified lactic acid bacteria obtained in step 2), inoculate 10 ml of MRS liquid ...
Embodiment 2
[0048] Lactic acid bacteria additive as verbena silage additive
[0049] The lactic acid bacteria additives of the experimental group 1-3 shown in Table 3 and the bacterial liquid of the control group 1-3 were respectively inoculated into the MRS liquid medium according to the inoculum amount, and sterilized distilled water was used as a blank control to obtain a bacterial strain additive solution, and the bacterial strain live The number of bacteria is set at 1×10 6 CFU / g FW;
[0050] Take "Ya'an" Verbena flat spike samples, cut into about 20mm in length, and obtain fresh samples. Each group shown in Table 2 takes 500g of fresh samples and puts them into plastic bags (25×35cm; Aodeju, China);
[0051] Spray the added solution of the strains shown in Table 3 evenly into the pasture sample, mix evenly, pack silage in a bag, vacuum seal it, leave it at room temperature (27-37° C.) and ferment it for 60 days, then unpack it to obtain a silage sample.
[0052] Sensory evaluation...
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more
PUM
Login to view more
Abstract
The invention belongs to the technical field of microorganisms, and particularly relates to a hemarthria herbage silage lactobacillus additive. The hemarthria herbage silage lactobacillus additive comprises a component A and a component B; the component A and the component B are each any one of lactobacillus rhamnosus 753, lactobacillus salivarius 358 and lactobacillus plantarum LP694, and the component A and the component B are different; the mass ratio of the component A to the component B is (1-2): (1-2); the preservation number of the lactobacillus rhamnosus 753 is CGMCC No.18233, the preservation number of the lactobacillus salivarius 358 is CGMCCNo.18232, and the preservation number of the lactobacillus plantarum LP694 is CGMCC No.15073. The lactobacillus additive disclosed by the invention is high in growth rate and high in acid production efficiency; the sensory quality of hemarthria herbage silage can be obviously improved; the lactic acid and acetic acid content of the hemarthria herbage silage can be significantly increased, and the content of harmful acids including butyric acid and the like is reduced, so that lactobacillus becomes dominant microflora, harmful bacteriain the hemarthria herbage silage are inhibited, and the ensiling quality of herbage is improved.
Description
technical field [0001] The invention belongs to the technical field of microbes, and in particular relates to a bullweed silage lactic acid bacteria additive and a preparation method thereof. Background technique [0002] Silage refers to a storage technology or method that compresses and seals fresh plants to isolate the stored green fodder from the outside air, resulting in internal hypoxia, resulting in anaerobic fermentation. Silage helps to improve the utilization value of feed, expand the source of feed, adjust the supply period of forage, make the total nutrient harvested per unit area reach the highest value, reduce the waste of nutrients, reduce labor intensity, improve work efficiency, and treat pests and diseases. [0003] Silage is essentially lactic acid bacteria fermented feed, and natural silage is fermented by lactic acid bacteria that exist on plants in nature. However, due to the low content of lactic acid bacteria on plants in nature, they only account fo...
Claims
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more
Application Information
Patent Timeline
Application Date:The date an application was filed.
Publication Date:The date a patent or application was officially published.
First Publication Date:The earliest publication date of a patent with the same application number.
Issue Date:Publication date of the patent grant document.
PCT Entry Date:The Entry date of PCT National Phase.
Estimated Expiry Date:The statutory expiry date of a patent right according to the Patent Law, and it is the longest term of protection that the patent right can achieve without the termination of the patent right due to other reasons(Term extension factor has been taken into account ).
Invalid Date:Actual expiry date is based on effective date or publication date of legal transaction data of invalid patent.
Login to view more 
Login to view more