Exosome separation method and exosome separation device

Abstract

The invention discloses an exosome separation method and an exosome separation device, which are improved on the basis of the existing ultracentrifugal separation method, an isopyknic buffer solution1xPBS buffer solution is added into a product after each time of centrifugation, finally, a supernatant is removed, precipitates are reserved, and the exosome separation method and the exosome separation device are improved on the basis of the existing exosome separation device. An automatic cover opening assembly is additionally arranged, the centrifugal cover opening time each time is saved, inaddition, through design of an exhaust device, under the effect of an elastic piece, the top end of a pressure maintaining pipe is tightly attached to a pressure relief cover, air pressure in a shellis kept within a constant range, and a sealing mechanism is arranged between a first protruding edge and a second protruding edge. The sealing performance of the device is greatly improved. In addition, optimization is performed on a rotating disc mechanism, and due to the fact that the number of mounting grooves in each sample containing groove is not less than three and the mounting grooves aredistributed in the radius of a disc body in an equal spacing manner, the device can at least meet the grouping contrast experiment of three groups of different samples.

Description

Technical field[0001]The present invention relates to the field of separation techniques of an exosomass, and is specifically a separation method of an exosoment body and a separation device thereof.Background technique[0002]Subjects refers to a small membrane (30-150 nm) containing complex RNA and protein. Today, it refers to a disk-shaped vesicle in diameter at 40-100 nm. In 1983, the primary bodies were found in the sheep net to the red cells, and 1987 Johnstone was named "EXOSOME". A variety of cells can secrete an epistem in a normal and pathological state. It is mainly derived from the polycycosomal particles formed by intracellular lysosomas, which are released to the extracellular matrix after fusion of the polycyclic bubbles and the cell membrane. All cultured cell types can secrete the epistemis, and the outer body is naturally present in the body fluid, including blood, saliva, urine, cerebrospinal fluid and milk. The accurate molecular mechanism of "carrier" and correspo...

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Problems solved by technology

[0003] The inventor found through research that the traditional ultracentrifugation separation method of exosomes is time-consuming and not conducive to the comparison of sample groups. How to invent a separation method of exosomes and its separation device to solve these problems has become a technical problem in the art. Problems that people need to solve

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