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Magnetic particle chemiluminiscence HER2 detection kit and preparation method thereof

A chemiluminescence method and detection kit technology, applied in the direction of measuring devices, scientific instruments, instruments, etc., can solve the problems of inability to judge HER2 status, difficult multiple detection, etc., to increase non-specific interference, avoid radioactive pollution, and convenient operation Effect

Pending Publication Date: 2021-03-26
JIANGSU ZECEN BIOTECH CO LTD
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] Since pathological samples are required for histological testing, it is impossible to judge the HER2 status of patients who have not obtained cancer histological pathology, and it is also difficult to detect multiple times

Method used

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  • Magnetic particle chemiluminiscence HER2 detection kit and preparation method thereof
  • Magnetic particle chemiluminiscence HER2 detection kit and preparation method thereof
  • Magnetic particle chemiluminiscence HER2 detection kit and preparation method thereof

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Embodiment 1

[0041] A magnetic particle chemiluminescence detection kit for HER2, comprising the following components: magnetic separation reagent (fluorescein isothiocyanate immunomagnetic particle) (Beijing Zecheng), fluorescein isothiocyanate-labeled anti-HER2 antibody, alkaline Phosphatase-labeled anti-HER2 antibody and calibrator (Gianbo Biotech).

[0042] Wherein, the preparation steps of fluorescein isothiocyanate-labeled anti-HER2 antibody are as follows:

[0043] (1) Measure 1 mg of anti-HER2 antibody (monoclonal paired antibody, Gambor Biotech);

[0044] (2) Calculated by the molecular weight of anti-HER2 antibody (150000) and molecular weight of fluorescein isothiocyanate (389.4), and weigh the fluorescence of isothiocyanate according to the molar ratio of anti-HER2 antibody to fluorescein isothiocyanate (1:30) Su (Tian Bo Bio);

[0045] (3) Weigh 5mg of fluorescein isothiocyanate and dissolve it in 0.05M pH 9.0 sodium carbonate buffer to a final concentration of 5mg / ml;

[004...

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Abstract

The invention discloses a magnetic particle chemiluminiscence method HER2 detection kit and a preparation method, and belongs to the technical field of biological immunity. The invention discloses anHER2 detection kit adopting a magnetic particle chemiluminiscence method. The HER2 detection kit comprises the following components: a magnetic separation reagent, a fluorescein isothiocyanate labeledanti-HER2 antibody, an alkaline phosphatase labeled anti-HER2 antibody and a calibrator. The kit adopts serological detection, has the characteristics of convenience, repeatable measurement, quantification, objectivity, real-time detection and the like, and is a supplement for histological detection; the whole reaction system is short in reaction time, radioactive contamination is avoided, the experiment operation process is simplified, the pain of a patient is relieved through the sampling mode, and operation is convenient.

Description

technical field [0001] The invention relates to the technical field of biological immunity, in particular to a magnetic particle chemiluminescent HER2 detection kit and a preparation method. Background technique [0002] Human epidermal growth factor receptor-2 (HER2), independently discovered by three research groups in the 1980s, is one of the most thoroughly studied breast cancer genes so far. The HER2 gene is a prognostic indicator for clinical treatment monitoring and an important target for the selection of tumor targeted therapy drugs. Serum HER2 is associated with tumor burden, histological HER2, and lymph node status in breast cancer patients, and may be an independent prognostic factor, which may have a certain impact on the efficacy of chemotherapy or endocrine therapy. [0003] The HER2 protein is usually expressed only in the fetus, and is expressed at a low level in very few tissues in adulthood. However, studies have shown that HER2 gene amplification / overex...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/574G01N33/535G01N33/533
CPCG01N33/57415G01N33/57488G01N33/535G01N33/533G01N2333/71G01N2333/82G01N2800/52
Inventor 陈海生刘振世杨志刚李婷婷
Owner JIANGSU ZECEN BIOTECH CO LTD
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