Application of a combinatorial knockout of rice disease-susceptible genes in the creation of broad-spectrum disease-resistant materials

Abstract

The invention discloses the application of combined knockout of rice susceptibility genes in the creation of broad-spectrum disease-resistant materials. The combination knockout of rice susceptibility genes through CRISPR / Cas9 gene editing technology can improve the resistance of rice to blast and bacterial blight disease capacity. The invention discloses three susceptibility-related gene combination knockout mutant materials of rice, and confirms that the bxp triple knockout mutant material not only improves the resistance of rice to blast fungus, but also improves the resistance to bacterial blight, and Compared with the single mutation, there was an additive effect on the disease resistance level of the triple mutant. While achieving broad-spectrum disease resistance, the bxp triple knockout mutant had no significant change in agronomic traits compared with the control.

Description

technical field [0001] The invention belongs to the field of genetic engineering, and in particular relates to the application of combined knockout of rice disease-susceptible genes in the creation of broad-spectrum disease-resistant materials. Background technique [0002] Plant diseases seriously affect the yield and quality of crops worldwide. In the process of agricultural production, crops are often infected by two or more pathogenic bacteria successively or simultaneously in the same growing season. Therefore, improving the broad-spectrum disease resistance of crops is of great significance to the high and stable yield of crops. Rice is one of the three major food crops in the world and the most important food crop in my country. Rice production plays a cornerstone role in my country's food security. Bacterial leaf blight (Bacteriale afblight) and rice blast (Riceblast) are considered to be one of the most serious bacterial diseases of rice and one of the most destruc...

AI Technical Summary

Problems solved by technology

For example, the knockout mutant materials of rice blast fungus susceptibility genes Bsrd1 and Pi21 have partially improved the disease resistance of rice to blast fungus, and the mutant materials of the susceptibility gene Xa5 of Xanthobacterium spp. disease resistance, but the disease resistance level of these three susceptibility genes is not enough to be used alone for the creation of disease-resistant materials

In addition, Pi21 and Xa5 are usually aggregated with other genes in the form of a single quantitative trait for molecular breeding of disease resistance, but this method takes a long time

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