A kit for increasing the number of canine ovary cumulus-oocyte complexes and its application

Abstract

The present invention relates to a kind of test kit and its application for increasing the number of canine ovary cumulus-oocyte complexes (Cumulus Oocyte Complexes, COCs), adding estradiol benzoate EB, cloprostinol PGF2α and novel Hormone preparation FG600 three kinds of hormones, and also established a simple hormone induction method that can make estrus Beagle dogs enter proestrus in a short period of time. This method can significantly increase the number of available COCs in dog ovaries. This method is still popular at home and abroad. This is the first time. The kit for increasing the number of canine ovary cumulus-oocyte complexes described in the present invention can make the dogs in estrus enter into proestrus within ten days, and the average number of COCs recovered from the ovary by puncture method is about 58 pieces, which is significantly higher than Normal estrus dogs (average about 33.7).

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to a kit for increasing the number of canine ovary cumulus-oocyte complexes and an application thereof. Background technique [0002] Beagle dogs are internationally recognized experimental animals and are widely used in various fields of scientific research. Domestic Beagle dog resources are imported from abroad. At present, foreign countries have relatively strict control over Beagle germplasm resources, so it is particularly important to preserve and develop domestic existing resources. At the same time, the genetic background of Beagle dogs is clear, and its genome is as high as 95.7% similar to humans. The construction of disease models using Beagle dogs as experimental materials has become a research hotspot for many scholars. Obtaining mature oocytes is a crucial step in the process of canine in vitro breeding work and disease model preparation. Compared with other mammals, due t...

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Problems solved by technology

[0005] Since 1939, scholars at home and abroad have conducted a lot of research on the method of inducing estrus and ovulation in dogs. According to relevant literature reports, exogenous hormones and drugs to induce estrus in dogs generally include: gonadotropin-releasing hormone, gonadotropin, prostaglandin, Estrogen and dopamine receptor agonists, etc., the combined use of several hormones is better than a single hormone, dopamine receptor agonists can achieve a good effect of inducing estrus, but the administration time is long (Kutzler M A. Induction and synchronization of estrus in dogs[J]. Theriogenology, 2005,64(3):766-775.), which limits the application of large-scale production

The new hormone preparation FG600 includes the new long-acting recombinant follicle-stimulating hormone rFSH, which has high stability, longer half-life, higher activity, cheaper price and easier access, and can be effectively applied to sows (Chen Zhilin, Chen Yuming, Yuan Meng, et al. The effect of new hormone preparation FG600 on the estrus synchronization and reproductive performance of gilts[J]. Swine Science, 2014,31(12):102-104.), but the application in dogs has not yet been found Related reports

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